LOCUS       sequence-47265-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Dittmer PJ, Miranda JG, Gorski JA, Palmer AE
  TITLE     Genetically encoded sensors to elucidate spatial distribution of 
            cellular zinc.
  JOURNAL   J Biol Chem. 2009 Jun 12;284(24):16289-97. Epub 2009 Apr 10.
  PUBMED    19363034
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2009-06-12"; volume: "284(24)"; pages: "16289-97. 
            Epub 2009 Apr 10"
COMMENT     Sequence Label: pcDNA-ZifCY3 (Ala4)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(22..732,733..735,736..738))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(64..85)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     413..432
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     672..693
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tggatatctg cagaattctt acttgtagag ctcgtccatg ccgagagtga tcccggcggc
       61 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      121 ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      181 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      241 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
      301 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      361 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      421 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      481 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcaca tcaggccgta
      541 gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      601 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      661 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
      721 ctcgcccttg ctcaccatga gctccttctg gcctgtagcg atgcggatag cgcgggtaag
      781 ctcatccgag cganaaaagc ggcgatcggc ggactcgaca gggg
//
