LOCUS       sequence-472655-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty lentiviral vector for expressing protein sequences fused to 
            GFP-6mer_oligomerization_domain, under a TRE3GV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  TITLE     Characterizing protein sequence determinants of nuclear condensates 
            by high-throughput pooled imaging with CondenSeq
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGFP_6
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2394..2743
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2438..2456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2474..2492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2510..2528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2546..2564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2582..2600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2618..2636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2682..2706
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4733..5446
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4763..4791)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5386..5406
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    5640..6228
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5693..5713)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6111..6122)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6279..6299)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6284..6613
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6464..6599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6667..7266
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6667..6686)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7163..7183
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7338..7571
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7643..7764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7680..7699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7734..7753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7804..7939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7959..7978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7960..7978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7988..8005)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7988..8004)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7997..8019)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8146..8601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8233..8252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8443..8464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8627..8731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8732..8800,8801..9592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8950..8969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9763..10351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10252..10271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atcgggttta
     2341 ttacagggac agcagagatc cagtttatcg atgaggccct ttcgtcttca ctcgagttta
     2401 ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt
     2461 atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt
     2521 gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta
     2581 ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt
     2641 atgtcgaggt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt
     2701 cagatcgcct ggagcaattc cacaacactt ttgtcttata cttacgcgtg acaaattgcc
     2761 cgggctgtga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     2821 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     2881 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     2941 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     3001 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     3061 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     3121 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     3181 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     3241 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     3301 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     3361 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     3421 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     3481 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     3541 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     3601 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     3661 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     3721 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     3781 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     3841 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     3901 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     3961 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     4021 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     4081 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     4141 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     4201 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     4261 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     4321 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     4381 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     4441 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     4501 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     4561 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     4621 agcttcttgc tcttttttgt acgtctcggg atccggagct accgcggagc cgcgtagcaa
     4681 acgtccgcgt gtgacgctgc cggcgtgtcc ggcgcatgga tccggagcta ccatgagtaa
     4741 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     4801 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     4861 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     4921 cctgacttat ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
     4981 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     5041 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     5101 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     5161 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     5221 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     5281 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     5341 acaatctaag ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     5401 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaaggtg gatctggagg
     5461 ttcaggtgga agtgcggccg gtaccggcag cggaggaggc gaaatcgcca agagcctgaa
     5521 ggaaatagcc aagtctctca aggagatcgc ctggagcctc aaagaaattg ccaaatccct
     5581 gaaaggctga actagttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca
     5641 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5701 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5761 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5821 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5881 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5941 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6001 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6061 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6121 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6181 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctgc
     6241 agatatccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc agttagggtg
     6301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6361 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6421 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6541 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6601 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     6661 gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgatga tgtccctagg
     6721 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6781 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6841 ctcgacatcg gcaaggtgtg ggtcgctgat gacggcgccg ccgtggcggt ctggaccacg
     6901 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6961 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     7021 aaggagcccg cgtggttcct ggccaccgtc ggcgtttcgc ccgaccacca gggcaagggt
     7081 ctgggcagcg ccgtcgtgct ccccggagtg gaagctgccg agcgcgccgg ggtgcctgcc
     7141 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     7201 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     7261 gcctagggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7321 aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt
     7381 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7441 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7501 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7561 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7621 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7741 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7801 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7861 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7921 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7981 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8041 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8101 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     8161 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8221 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8281 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8341 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8401 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8461 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8521 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8581 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8641 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8701 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8761 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8821 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8881 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8941 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     9001 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9061 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9121 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9181 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9241 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9301 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9361 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9421 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9481 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9541 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9601 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9661 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9841 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9901 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    10021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10381 cctggccttt tgctggcctt ttgctcacat g
//
