LOCUS       sequence-472656-       11061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty lentiviral vector for expressing protein sequences fused to 
            GFP and FTH1 (24-mer), under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11061)
  TITLE     Characterizing protein sequence determinants of nuclear condensates 
            by high-throughput pooled imaging with CondenSeq
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV_GFP_24
FEATURES             Location/Qualifiers
     source          1..11061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2710..2913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2864..2884
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2910..2934
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4924..5637
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4954..4982)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5577..5597
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    6290..6878
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6343..6363)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6761..6772)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6929..6949)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6934..7263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7114..7249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7317..7916
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7317..7336)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7813..7833
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7988..8221
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8293..8414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8330..8349)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8384..8403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8454..8589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8609..8628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8610..8628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8638..8655)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8638..8654)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8647..8669)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8796..9251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8883..8902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9093..9114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9277..9381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9382..9450,9451..10242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9600..9619)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10413..11001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10902..10921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atcgggttta
     2341 ttacagggac agcagagatc cagtttatcg atgaggccct ttcgtcttca ctcgagggag
     2401 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     2761 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2821 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2881 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcacgcgt
     2941 gacaaattgc ccgggctgtg agacggttgt aaatgagcac acaaaataca catgctaaaa
     3001 tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat
     3061 caataattag aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa
     3121 aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc
     3181 acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt caaatgttct
     3241 aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca ttagtttaac
     3301 atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa attctaatcc
     3361 agcttcaaca gctatttcac aagctttcat gatttcttct tttgttaata aacaattttc
     3421 cataatacat ttaacaacat gtgatccagc tgcttttttt acagctttca tgtcttctaa
     3481 aactaattca taatttttgt cttttaatgc accaatattt aataccatat caatttctgt
     3541 tgcaccatct ttaattgctt cagaaacttc gaatgctttt gtagctgttg tgcatgcacc
     3601 tagaggaaaa cctacaacat ttgttattcc tacatttgtg ccttttaata attctttaca
     3661 atagcttgtt caatatgaat taacacaaac tgttgcaaaa tcaaattcaa ttgcttcatc
     3721 acataattgt ttaatttcag ctttcgtagc atcttgtttt aataatgtgt gatctatata
     3781 tttgtttagt ttcatttttt ctcctatata ttcattttta attttaattc tttaataatt
     3841 tcgtctactt taactttagc gttttgaaca gattcaccaa cacctataaa ataaattttt
     3901 agtttaggtt cagttccact tgggcgaaca gcaaatcatg acttatcttc taaataaaat
     3961 tttagtaagt cttgtcctgg catattatac attccatcga tgtagtcttc aacattaaca
     4021 actttaagtc cagcaatttg agttaagggt gttgctctca atgatttcat taatggttca
     4081 atttttaatt tcttttcttc tggtttaaaa ttcaagttta aagtgaaagt gtaatatgca
     4141 cccatttctt taaataaatc ttctaaatag tctactaatg ttttattttg ttttttataa
     4201 aatcaagcag cctctgctat taatatagaa gcttgtattc catctttatc tctagctgag
     4261 tcatcaatta catatccata actttcttca taagcaaaaa caaaatttaa tccgttatct
     4321 tcttctttag caatttctct acccattcat ttaaatccag ttaaagtttt tacaatatta
     4381 actccatatt tttcatgagc gattctatca cccaaatcac ttgttacaaa acttgaatat
     4441 agagccggat tttttggaat gctatttaag cgttttagat ttgataattt tcaatcaatt
     4501 aaaattggtc ctgtttgatt tccatctaat cttacaaaat gaccatcatg ttttattgcc
     4561 attccaaatc tgtcagcatc tgggtcattc ataataataa tatctgcatc atgtttaata
     4621 ccatattcaa gcggtatttt tcatgcagga tcaaattctg gatttggatt tacaacattt
     4681 ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa taacgttata tcctgattca
     4741 cgtaatattt ttggggtaaa tttagttcct gttccattaa ctgcgctaaa aataattttt
     4801 aaatcttttt tagcttcttg ctcttttttg tacgtctcgg gatccggagc taccgcggag
     4861 ccgcgtagca aacgtccgcg tgtgacgctg ccggcgtgtc cggcgcatgg atccggagct
     4921 accatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     4981 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     5041 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     5101 cttgtcacta ccctgactta tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa
     5161 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     5221 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     5281 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     5341 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     5401 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     5461 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     5521 tacctgtcca cacaatctaa gctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     5581 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaaggt
     5641 ggatctggag gttcaggtgg aagtgcggcc ggtaccggca gcggaggaat gacgaccgcg
     5701 tccacctcgc aggtgcgcca gaactaccac caggactcag aggccgccat caaccgccag
     5761 atcaacctgg agctctacgc ctcctacgtt tacctgtcca tgtcttacta ctttgaccgc
     5821 gatgatgtgg ctttgaagaa ctttgccaaa tactttcttc accaatctca tgaggagagg
     5881 gaacatgctg agaaactgat gaagctgcag aaccaacgag gtggccgaat cttccttcag
     5941 gatatcaaga aaccagactg tgatgactgg gagagcgggc tgaatgcaat ggagtgtgca
     6001 ttacatttgg aaaaaaatgt gaatcagtca ctactggaac tgcacaaact ggccactgac
     6061 aaaaatgacc cccatttgtg tgacttcatt gagacacatt acctgaatga gcaggtgaaa
     6121 gccatcaaag aattgggtga ccacgtgacc aacttgcgca agatgggagc gcccgaatct
     6181 ggcttggcgg aatatctctt tgacaagcac accctgggag acagtgataa tgaaagctga
     6241 actagttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct
     6301 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6361 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6421 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6481 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6541 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6601 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6661 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6721 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6781 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6841 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctgc agatatccgg
     6901 ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6961 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7021 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7081 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7141 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7201 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7261 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gccaccatga
     7321 ccgagtacaa gcccacggtg cgcctcgcca cccgcgatga tgtccctagg gccgtacgca
     7381 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     7441 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7501 gcaaggtgtg ggtcgctgat gacggcgccg ccgtggcggt ctggaccacg ccggagagcg
     7561 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7621 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7681 cgtggttcct ggccaccgtc ggcgtttcgc ccgaccacca gggcaagggt ctgggcagcg
     7741 ccgtcgtgct ccccggagtg gaagctgccg agcgcgccgg ggtgcctgcc ttcctggaga
     7801 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7861 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagggta
     7921 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7981 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
     8041 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8101 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8161 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8221 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8281 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8341 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8401 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     8461 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8521 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8581 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     8641 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8701 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     8761 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     8821 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8881 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8941 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9001 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9061 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9121 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9181 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9241 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9301 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9361 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9421 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9481 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9541 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9601 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9661 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9721 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9781 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9841 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9901 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     9961 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10021 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10081 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10141 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10201 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10261 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10321 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10681 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10741 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10801 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10861 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10921 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10981 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11041 tgctggcctt ttgctcacat g
//
