LOCUS       sequence-472657-        9024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ME-ABE ABE7.10-HRHSK with SpCas9 (NG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9024)
  TITLE     Directed Evolution Reversion Analysis Produces Minimally Evolved 
            Adenine Base Editor Variants with Improved Efficiency and Precision
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pME270
FEATURES             Location/Qualifiers
     source          1..9024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1062..1286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1275..1292
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1315..1332)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1341..1361)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1374..2081)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1649..1668)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1918..1936
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(2082..2138)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2148..2168)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2211..6311)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6906..6926)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6975..6994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6976..6994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7036..7239)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7065..7085)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7240..7619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7791..7810
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7889..7993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7994..8062,8063..8854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8212..8231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
     1321 atggtgatga tgaccggtta gactttcctc ttcttcttgg gagaaccacc agacttgtac
     1381 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg
     1441 gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc
     1501 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
     1561 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
     1621 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
     1681 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
     1741 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     1801 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
     1861 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg
     1921 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     1981 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
     2041 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca taggtccagg gttctcctcc
     2101 acgtctccag cctgcttcag caggctgaag ttagtagctc cgcttccgac tttcctcttc
     2161 ttcttgggct cgaattcgct gccgtcggcg gttctttttg agccgccaga gtcacctccc
     2221 agctgagaca ggtcgatccg tgtctcgtac aggccggtga tgctctggtg gatcagggtg
     2281 gcgtccagca cctctttggt gctacggtac accttccggt cgatggtggt gtcaaagtac
     2341 ttgaaggccc taggggctcc cagattggtc agggtaaaca ggtggatgat attctcggcc
     2401 tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt gtccagatta
     2461 gcgtcggcca ggatcactct cttggagaac tcgctgatct gctcgatgat ctcgtccagg
     2521 tagtgcttgt gctgttccac aaacagctgt ttctgctcat tatcctcggg ggagcccttc
     2581 agcttctcat agtggctggc caggtacagg aagttcacat atttggaggg cagggccagt
     2641 tcgtttccct tctgcaggaa ccgggcagag gccagcattc tcttccggcc gttttccagc
     2701 tcgaacaggg agtacttagg cagcttgatg atcaggtcct ttttcacttc tttgtagccc
     2761 ttggcttcca gaaagtcgat gggattcttc tcgaagctgc ttctttccat gatggtgatc
     2821 cccagcagct ctttcacact cttcagtttc ttggacttgc ccttttccac tttggccacc
     2881 accagcacag aataggccac ggtggggctc acgaagccgc cgtacttctt agggtcccag
     2941 tccttctttc tggcgatcag cttatcgctg ttcctcttgg gacggataga ctctttgctg
     3001 aagccgcctg tctgcacctc ggtctttttc acgatattca cttggggcat gctcagcact
     3061 ttccgcacgg tggcaaaatc ccggccctta tcccacacga tctccccggt ttcgccgttt
     3121 gtctcgatca gaggccgctt ccggatctcg ccgttggcca gggtaatctc ggtcttgaaa
     3181 aagttcatga tgttgctgta gaagaagtac ttggcggtag ccttgccgat ttcctgctcg
     3241 ctcttggcga tcatcttccg cacgtcgtac accttgtagt cgccgtacac gaactcgctt
     3301 tccagcttag ggtacttttt gatcagggcg gttcccacga cggcgttcag gtaggcgtcg
     3361 tgggcgtggt ggtagttgtt gatctcgcgc actttgtaaa actggaaatc cttccggaaa
     3421 tcggacacca gcttggactt cagggtgatc actttcactt cccggatcag cttgtcattc
     3481 tcgtcgtact tagtgttcat ccgggagtcc aggatctgtg ccacgtgctt tgtgatctgc
     3541 cgggtttcca ccagctgtct cttgatgaag ccggccttat ccagttcgct caggccgcct
     3601 ctctcggcct tggtcagatt gtcgaacttt ctctgggtaa tcagcttggc gttcagcagc
     3661 tgccgccagt agttcttcat cttcttcacg acctcttcgg agggcacgtt gtcgctcttg
     3721 ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga tggagtcgtc cttcagaaag
     3781 ctctgaggca cgatatggtc cacatcgtag tcggacagcc ggttgatgtc cagttcctgg
     3841 tccacgtaca tatcccgccc attctgcagg tagtacaggt acagcttctc gttctgcagc
     3901 tgggtgtttt ccacggggtg ttctttcagg atctggctgc ccagctcttt gatgccctct
     3961 tcgatccgct tcattctctc gcggctgttc ttctgtccct tctgggtggt ctggttctct
     4021 ctggccattt cgatcacgat gttctcgggc ttgtgccggc ccatcacttt cacgagctcg
     4081 tccaccacct tcactgtctg caggatgccc ttcttaatgg cggggctgcc ggccagattg
     4141 gcaatgtgct cgtgcaggct atcgccctgg ccggacacct gggctttctg gatgtcctct
     4201 ttaaaggtca ggctgtcgtc gtggatcagc tgcatgaagt ttctgttggc gaagccgtcg
     4261 gacttcagga aatccaggat tgtcttgccg gactgcttgt cccggatgcc gttgatcagc
     4321 ttccggctca gcctgcccca gccggtgtat ctccgccgct tcagctgctt catcactttg
     4381 tcgtcgaaca ggtgggcata ggttttcagc cgttcctcga tcatctctct gtcctcaaac
     4441 agtgtcaggg tcagcacgat atcttccaga atgtcctcgt tttcctcatt gtccaggaag
     4501 tccttgtcct tgataatttt cagcagatcg tggtatgtgc ccagggaggc gttgaaccga
     4561 tcttccacgc cggagatttc cacggagtcg aagcactcga ttttcttgaa gtagtcctct
     4621 ttcagctgct tcacggtcac tttccggttg gtcttgaaca gcaggtccac gatggccttt
     4681 ttctgctcgc cgctcaggaa ggcgggcttt ctcattccct cggtcacgta tttcactttg
     4741 gtcagctcgt tatacacggt gaagtactcg tacagcaggc tgtgcttggg cagcaccttc
     4801 tcgttgggca ggttcttatc gaagttggtc atccgctcga tgaagctctg ggcggaagcg
     4861 cccttgtcca ccacttcctc gaagttccag ggggtgatgg tttcctcgct ctttctggtc
     4921 atccaggcga atctgctgtt tcccctggcc agagggccca cgtagtaggg gatgcggaag
     4981 gtcaggatct tctcgatctt ttcccggttg tccttcagga atgggtaaaa atcttcctgc
     5041 cgccgcagaa tggcgtgcag ctctcccagg tggatctggt gggggatgct gccgttgtcg
     5101 aaggtccgct gcttccgcag caggtcctct ctgttcagct tcacgagcag ttcctcggtg
     5161 ccgtccatct tttccaggat gggcttgatg aacttgtaga actcttcctg gctggctccg
     5221 ccgtcaatgt agccggcgta gccgttcttg ctctggtcga agaaaatctc tttgtacttc
     5281 tcaggcagct gctgccgcac gagagctttc agcagggtca ggtcctggtg gtgctcgtcg
     5341 tatctcttga tcatagaggc gctcaggggg gccttggtga tctcggtgtt cactctcagg
     5401 atgtcgctca gcaggatggc gtcggacagg ttcttggcgg ccagaaacag gtcggcgtac
     5461 tggtcgccga tctgggccag caggttgtcc aggtcgtcgt cgtaggtgtc cttgctcagc
     5521 tgcagtttgg catcctcggc caggtcgaag ttgctcttga agttgggggt caggcccagg
     5581 ctcagggcaa tcaggtttcc gaacaggcca ttcttcttct cgccgggcag ctgggcgatc
     5641 agattttcca gccgtctgct cttgctcagt ctggcagaca ggatggcctt ggcgtccacg
     5701 ccgctggcgt tgatggggtt ttcctcgaac agctggttgt aggtctgcac cagctggatg
     5761 aacagcttgt ccacgtcgct gttgtcgggg ttcaggtcgc cctcgatcag gaagtggccc
     5821 cggaacttga tcatgtgggc cagggccaga tagatcagcc gcaggtcggc cttgtcggtg
     5881 ctgtccacca gtttctttct caggtggtag atggtggggt acttctcgtg gtaggccacc
     5941 tcgtccacga tgttgccgaa gatggggtgc cgctcgtgct tcttatcctc ttccaccagg
     6001 aaggactctt ccagtctgtg gaagaagctg tcgtccacct tggccatctc gttgctgaag
     6061 atctcttgca gatagcagat ccggttcttc cgtctggtgt atcttcttct ggcggttctc
     6121 ttcagccggg tggcctcggc tgtttcgccg ctgtcgaaca gcagggctcc gatcaggttc
     6181 ttcttgatgc tgtgccggtc ggtgttgccc agcaccttga atttcttgct gggcaccttg
     6241 tactcgtcgg tgatcacggc ccagcccaca gagttggtgc cgatggccag gccgatgctg
     6301 tacttcttgt ctgacccccc gctgctgccc ccgctgcttt caggtgttgc gctctcgctt
     6361 gtgccaggtg tctcagagcc agaggagcct ccgctagatc ctccggagtc ggtggagctc
     6421 tgggccttct tctgagcctt gaacacctgt ctaggcatcc gaaagaaata agacagcagg
     6481 gcggcacatt catctgccag gattccctcg gtaatttcga cgcggtgatt catgccgggg
     6541 tggtgcagca cgtccatcag ggagcctgcg gcgccggttt ttgcgttcct cacgccaaac
     6601 accacgcggc cgatcctaga gtggatcatg gcgccggcgc acatcacgca aggctcgaat
     6661 gtcacgtaca gggtggcgtc aatcagtctg tagttctgca tgaccaggcc gccctgtctc
     6721 agggccataa tttcggcatg ggctgttggg tcgtgccggc cgatggctct gttccagccc
     6781 tcgccgatca ctctattgtt gtgcaccagc acggctccca caggcacctc cctctcatcg
     6841 cgtgccctct tggccagggt cagggcatgt ctcatccagt actcgtggga aaactccacc
     6901 tcagagactt tccgcttctt ctttggtgac tcgaactcgc ttccgtcggc tgtccgtttc
     6961 atggtggcgg ctctccctat agtgagtcgt attagcggcc gcggatctct agcggatctg
     7021 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     7081 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     7141 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     7201 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     7261 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     7321 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     7381 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     7441 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     7501 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     7561 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     7621 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     7681 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     7741 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
     7801 gcagattgta ctgagagtcg accctagggg atcgggagat cgatctcccg atccgtcgac
     7861 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7921 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7981 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8041 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8101 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8161 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8221 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8281 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8341 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8401 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8461 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8521 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8581 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8641 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8701 tgagcgtggg tcccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8761 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8821 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8881 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8941 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag tgtcagaccc
     9001 cgtagaaaag atcaaaggat cttc
//
