LOCUS       sequence-472659-        5911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IVT mRNA of human gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Soto DC, Uribe-Salazar JM, Kaya G, Valdarrago R, Sekar A, Haghani 
            NK, Hino K, La GN, Mariano NAF, Ingamells C, Baraban AE, Turner TN, 
            Green ED, Simo S, Quon G, Andres AM, Dennis MY
  TITLE     Gene expansions contributing to human brain evolution.
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.09.26.615256. doi: 
            10.1101/2024.09.26.615256.
  PUBMED    39386494
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.09.26.615256. doi: 
            10.1101/2024.09.26.615256."
COMMENT     Sequence Label: PDZK1P1_pGCS1
FEATURES             Location/Qualifiers
     source          1..5911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1143..1277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1180..1199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1234..1253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3123..3147)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3187..3205)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3188..3205)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(3240..4224)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     rep_origin      4295..4750
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4382..4401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4592..4613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4776..4880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4881..4949,4950..5741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5099..5118)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgg gcccaatgca ttggcgccgc ggccgcgaat
     1081 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1141 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1261 cttatcatgt ctggatctac gtaatacgac tcactatagt tctagaacca ctttgtacaa
     1321 gaaagctggg tgttaatggt atttattcta gcaaccacaa cattgttaca aaagcacaat
     1381 tttaataggc ttatctgcta agatgctttt ataagcagct gtcacctata cagagttatg
     1441 aatcatcttt ggtgctcaag gaacctgtag aagtaagaga catcatcata cagagaaatg
     1501 tagttaagtt gaagcttgga aaagatcaca tgaaaaaaat ctagctcttg ccttatctct
     1561 tcctaagtta agcataaatt agccgtctgc aatagccgcc tgtaagacaa atgataacag
     1621 aagacaatca cacatggtga atggtttcca gtggagtttt tcttctaaag agacagtaaa
     1681 caggtcgcaa ctcattcttt gagaaaggat tcctattaaa tacccagaaa cagctatcaa
     1741 ataaacagcc aaagctatta cttgttttca tcacatctct gtatcttcag aattggaaga
     1801 agaatgtgag gctgtactgt gggcccgttc ttttgccatg tgcgagtcat gctttgactc
     1861 caccactatt ccttctttag aatctggagg ggtgtcaagt ggatcagcca gggaggaaac
     1921 aatagggatt ttcttagctt ggaaataatc ataggccttc tttccacaga ctagaagtgt
     1981 gacattcttc ccactgctct ggattctatc caccaccttc tcatagggtt catctagcac
     2041 attcacccca ttcacttcaa tgatgacatc ctcatcctct agcccagcca agtcagcagg
     2101 accgcccttc tgtacctctt tgatgaatga gcctggcaga ccccgaatcg catttaagtg
     2161 aaagccatag ccattttcac ctttagccag cctgcagagt ttaggcttat gatcttcttc
     2221 ctctgtagta tctggtggac ttgagacttc cagagaagtg ggagtaggag ctggagcctc
     2281 cttgacagag ccattgggca gttcttgact ttgatagtag agaaatggag aaaaatgagc
     2341 cagtctgtac atgttgtccg tctctttgtc taccaccaac agtgaagtct gatctccacc
     2401 ctttctaatc atttctacca cactgtcatg atccagggtt tccacagact cgccgttgac
     2461 agcaactacc agatcattgt tcttcaagcc agcctcctct gctggacttc cagaatctat
     2521 gtccttgatg atttgacctt tctgttctga gcctgccctc agatagaaac catagccatt
     2581 gcttcctttc ttcatctcca caattcgggg ctggtggggt aacagtttca aactggctgt
     2641 ttctcttttg aattgtatct tctgctcaac atgacgcttg tcagtttctt tgtccaccag
     2701 caggaacatg acacggcttc ctgacttctt caccttttca accacttcct catggctggc
     2761 atcctctaca ttctctccat tcacttcaat caagtgatca tcagccagaa ctccagctct
     2821 catagccaca ccttgaggtg taatatcagt catgtatacc ccctttttac cttggacagt
     2881 tttcagagag aagccatggc tgcctccctc cttcacgaga tagcagagcc ggggctgggt
     2941 ccaagtttgc acacctccat tcatcacagg ggaaagtata ttatcactca aaccttgctc
     3001 cttctgactt tgacccaact ctttcaagtc cacccgtgtt ttcactgctt tctcatagga
     3061 atccccatcc agaactagta aagtcactga attcccactc tttctgacca gatccacaac
     3121 ctagcctgct tttttgtaca aacttgtggc cggccggatc ctgcaaaaag aacaagtagc
     3181 ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc agaatggcgg
     3241 ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc gcccatttac
     3301 gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg gggatgtact
     3361 tggcagccat cgcgggccat ttaccgccat tgacgtcaat gggagtactg ccaatgtacc
     3421 ctggcgtact tccaatagta atgtacttgc caagttacta ttaatagata ttgatgtact
     3481 gccaagtggg ccatttaccg tcattgacgt caataggggg cgtgagaacg gatatgaatg
     3541 ggcaatgagc catcccattg acgtcaatgg tgggtggtcc tattgacgtc aatgggcatt
     3601 gagccaggcg ggccatttac cgtaattgac gtcaatgggg gaggcgccat atacgtcaat
     3661 aggaccgccc atatgacgtc aataggaaag accatatata gagaccattg acgtcaatgg
     3721 gggagtggct atgggcggta ttaggaagcc ccatatatgg tatatgggac cgcccattgg
     3781 gaggggctat ctacgtcaat aggaaaaccc atatatggaa tactatatgg catagggcca
     3841 atacatagta ttgaacctgg ccaatagcca tattggcata gggccatatt ggatattgcc
     3901 tatatattga tcctggcata tagccaatat ggccgccatt attggcacca tgccaattca
     3961 atatggcgga cttggcacag ggccaagacc cctcctcaaa cttggcaccg tgccaagtag
     4021 acccctcccc agttggcaca gtgccaggtc cgccatattg aattggcatg gtgccaagtc
     4081 cgccatattg aattggctgg ggtccagatc caccatattg aattggcacg agtccatata
     4141 cgccatattg aattggcaca ggtccagatc caccatattg aattggcatg agtccatata
     4201 cgccatattg aattggcggt cgactggcgt aatagcgaag aggcccgcac cgatcgccct
     4261 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4321 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4381 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4441 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4501 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4561 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4621 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4681 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4741 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5101 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5401 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5761 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5821 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5881 cagaccccgt agaaaagatc aaaggatctt c
//
