LOCUS       sequence-472672-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty lentiviral vector for expressing protein sequences fused to 
            GFP, under a TRE3GV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  TITLE     Characterizing protein sequence determinants of nuclear condensates 
            by high-throughput pooled imaging with CondenSeq
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGFP_1
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2394..2743
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2438..2456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2474..2492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2510..2528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2546..2564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2582..2600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2618..2636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2682..2706
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4733..5446
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4763..4791)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5386..5406
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    5499..6087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5552..5572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5970..5981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6138..6158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6143..6472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6323..6458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6526..7125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6526..6545)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7022..7042
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7197..7430
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7502..7623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7539..7558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7593..7612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7663..7798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7818..7837)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7819..7837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7847..7864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7847..7863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7856..7878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8005..8460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8092..8111)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8302..8323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8486..8590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8591..8659,8660..9451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8809..8828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9622..10210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10111..10130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg atcgggttta
     2341 ttacagggac agcagagatc cagtttatcg atgaggccct ttcgtcttca ctcgagttta
     2401 ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt
     2461 atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt
     2521 gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta
     2581 ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt
     2641 atgtcgaggt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt
     2701 cagatcgcct ggagcaattc cacaacactt ttgtcttata cttacgcgtg acaaattgcc
     2761 cgggctgtga gacggttgta aatgagcaca caaaatacac atgctaaaat attatattct
     2821 atgaccttta taaaatcaac caaaatcttc tttttaataa ctttagtatc aataattaga
     2881 atttttatgt tcctttttgc aaacttttaa taaaaatgag caaaataaaa aaacgctagt
     2941 tttagtaact cgcgttgttt tcttcacctt taataatagc tactccacca cttgttccta
     3001 agcggtcagc tcctgcttca atcatttttt gagcatcttc aaatgttcta actccaccag
     3061 ctgctttaac taaagcattg tctttaacaa ctgacttcat tagtttaaca tcttcaaatg
     3121 ttgcacctga ttttgaaaat cctgttgatg ttttaacaaa ttctaatcca gcttcaacag
     3181 ctatttcaca agctttcatg atttcttctt ttgttaataa acaattttcc ataatacatt
     3241 taacaacatg tgatccagct gcttttttta cagctttcat gtcttctaaa actaattcat
     3301 aatttttgtc ttttaatgca ccaatattta ataccatatc aatttctgtt gcaccatctt
     3361 taattgcttc agaaacttcg aatgcttttg tagctgttgt gcatgcacct agaggaaaac
     3421 ctacaacatt tgttattcct acatttgtgc cttttaataa ttctttacaa tagcttgttc
     3481 aatatgaatt aacacaaact gttgcaaaat caaattcaat tgcttcatca cataattgtt
     3541 taatttcagc tttcgtagca tcttgtttta ataatgtgtg atctatatat ttgtttagtt
     3601 tcattttttc tcctatatat tcatttttaa ttttaattct ttaataattt cgtctacttt
     3661 aactttagcg ttttgaacag attcaccaac acctataaaa taaattttta gtttaggttc
     3721 agttccactt gggcgaacag caaatcatga cttatcttct aaataaaatt ttagtaagtc
     3781 ttgtcctggc atattataca ttccatcgat gtagtcttca acattaacaa ctttaagtcc
     3841 agcaatttga gttaagggtg ttgctctcaa tgatttcatt aatggttcaa tttttaattt
     3901 cttttcttct ggtttaaaat tcaagtttaa agtgaaagtg taatatgcac ccatttcttt
     3961 aaataaatct tctaaatagt ctactaatgt tttattttgt tttttataaa atcaagcagc
     4021 ctctgctatt aatatagaag cttgtattcc atctttatct ctagctgagt catcaattac
     4081 atatccataa ctttcttcat aagcaaaaac aaaatttaat ccgttatctt cttctttagc
     4141 aatttctcta cccattcatt taaatccagt taaagttttt acaatattaa ctccatattt
     4201 ttcatgagcg attctatcac ccaaatcact tgttacaaaa cttgaatata gagccggatt
     4261 ttttggaatg ctatttaagc gttttagatt tgataatttt caatcaatta aaattggtcc
     4321 tgtttgattt ccatctaatc ttacaaaatg accatcatgt tttattgcca ttccaaatct
     4381 gtcagcatct gggtcattca taataataat atctgcatca tgtttaatac catattcaag
     4441 cggtattttt catgcaggat caaattctgg atttggattt acaacatttt taaatgtttc
     4501 atcttcaaat gcatgctctt caacctcaat aacgttatat cctgattcac gtaatatttt
     4561 tggggtaaat ttagttcctg ttccattaac tgcgctaaaa ataattttta aatctttttt
     4621 agcttcttgc tcttttttgt acgtctcggg atccggagct accgcggagc cgcgtagcaa
     4681 acgtccgcgt gtgacgctgc cggcgtgtcc ggcgcatgga tccggagcta ccatgagtaa
     4741 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     4801 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     4861 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     4921 cctgacttat ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
     4981 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     5041 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     5101 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     5161 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     5221 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     5281 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     5341 acaatctaag ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     5401 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaatgaa ctagttgtac
     5461 aaagtggttg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
     5521 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5581 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5641 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5701 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5761 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5821 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5881 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     5941 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6001 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6061 ggatctccct ttgggccgcc tccccgcctg gaattctgca gatatccggt tagtaatgag
     6121 tttggaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6481 gggagcttgt atatccattt tcggatctga tcagcacgtg ccaccatgac cgagtacaag
     6541 cccacggtgc gcctcgccac ccgcgatgat gtccctaggg ccgtacgcac cctcgccgcc
     6601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6721 gtcgctgatg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6781 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6841 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6901 gccaccgtcg gcgtttcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6961 cccggagtgg aagctgccga gcgcgccggg gtgcctgcct tcctggagac ctccgcgccc
     7021 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7081 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagggtac ctttaagacc
     7141 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7201 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7261 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7321 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7381 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7441 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7501 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7621 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7681 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7741 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7801 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     7861 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7921 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7981 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8161 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8221 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8341 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8401 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     8461 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8521 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8581 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8641 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8701 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8761 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8821 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8881 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8941 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9001 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9061 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9121 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9181 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9241 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9301 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9361 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9421 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9481 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9541 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9601 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9661 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9721 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9781 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9841 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9901 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9961 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10021 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10081 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10141 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10201 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10261 tgctcacatg
//
