LOCUS       sequence-47268-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pRosa26 R1-ccdB-R2 RexNeo PI-SceI
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..33
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15..33
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    140..364
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     353..370
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          438..503
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 attcgccagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac catctatgtc
       61 gggtgtggag aaagaggtaa tgaaatggca ggtaccgggc cccccctcga gccccagctg
      121 gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct
      181 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
      241 ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct
      301 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
      361 acagtcgagg ctgatcagcg agctctagga tctgcattcc accactgctc ccattcatca
      421 gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt aacacattat
      481 acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat
      541 tatgtcacac cacagaagta aggttccttc acaaagagat cgcctgacac gatttcctgc
      601 acaggcttga gccatatact catacatcgc atcttggcca cgttttccac gggtttcaaa
      661 attaatctca agttctacgc ttaacgcttt cgcctgttcc cagttattaa tatattcaac
      721 gctagaactc ccctcagcga agggaaggct gagcactaca cgcgaagcac catcaccgaa
      781 ccttttgata aactcttccg ttccgacttg ctccatcaac ggttcagtga gacttaaacc
      841 taactctttc ttaatagttt cg
//
