LOCUS       sequence-472690-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of structurally predicted WWE domain of DTX2 with EGFP on
            C terminal .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Al-Rahahleh RQ, Roos WP, Saville KM, Andrews JF, Wu Z, Koczor CA, 
            Prakash A, Sobol RW
  TITLE     Overexpression of the WWE domain of RNF146 modulates 
            poly-(ADP)-ribose dynamics at sites of DNA damage.
  JOURNAL   DNA Repair (Amst). 2025 Jun;150:103845. doi: 
            10.1016/j.dnarep.2025.103845. Epub 2025 May 21.
  PUBMED    40403420
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Repair 
            (Amst)."; date: "2025-06"; volume: "150:103845. doi"; pages: " 
            10.1016/j.dnarep.2025.103845. Epub 2025 May 21"
COMMENT     Sequence Label: pLV-EF1A-DTX2-1-178-EGFP-Hygro
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3753..3755,3756..3758,3759..4472)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3753..3755,3756..3758,3759..4469)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3798..3819)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4059..4078)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4406..4427
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4473..4497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4515..5103
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4568..4588)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4986..4997)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5134..5633
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5169..5192)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5566..5585
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5650..6675
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5650..6675
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6747..6980
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7052..7173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7089..7108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7143..7162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7213..7348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7368..7387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7369..7387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7397..7414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7397..7413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7406..7428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7555..8010
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7642..7661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7852..7873
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8036..8140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8141..8209,8210..9001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8359..8378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9172..9760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9661..9680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9914..9931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10048..10069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10084..10089,10090..10107,10108..10114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10122..10138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10127..10149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10146..10162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10146..10162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10181..10201
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10183..10201
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg atggccatgg
     3181 ctccctcccc gagtctggtg caggtgtata ccagccctgc cgccgtggct gtctgggagt
     3241 ggcaggacgg gctgggaacc tggcaccctt atagcgccac cgtctgttcc tttatcgagc
     3301 agcagtttgt gcagcagaag ggccagcgat ttggcctggg gtctctggcc cattctatcc
     3361 ctttgggaca ggccgaccct tccctcgctc cctacatcat cgatctgcct agctggacac
     3421 agttcaggca ggacaccggg acaatgcgcg ccgtgaggag gcatctgttt ccacagcata
     3481 gcgctccagg aaggggcgtg gtgtgggagt ggctgtcaga tgacgggtca tggaccgcct
     3541 acgaggcctc tgtgtgcgat tatctggagc agcaggtggc caggggaaac cagctggtgg
     3601 acctggcccc tctgggatat aattataccg tgaattacac tacacacacc cagactaata
     3661 agactagcag tttctgtcgg agtgtgagaa gacaggctgg cccctctgga ggaggcggat
     3721 caggcggggg aggaagtgga ggaggtggaa gcatggtgag caagggcgag gagctgttca
     3781 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3841 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3901 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3961 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4021 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4081 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4141 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4201 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4261 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4321 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4381 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4441 tcactctcgg catggacgag ctgtacaagt aaacccagct ttcttgtaca aagtggtgat
     4501 aatcgaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4561 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4621 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4681 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4741 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4801 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4861 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4921 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4981 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5041 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5101 cgcatcggga attcccgcgg ttcgaattct accgggtagg ggaggcgctt ttcccaaggc
     5161 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     5221 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     5281 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     5341 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     5401 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     5461 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     5521 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     5581 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcac
     5641 gtggccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     5701 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     5761 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     5821 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     5881 cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg tgcacagggt
     5941 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     6001 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     6061 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     6121 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     6181 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     6241 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     6301 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     6361 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     6421 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     6481 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     6541 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     6601 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     6661 agggcaaagg aatagggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6721 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6781 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6841 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6901 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6961 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7021 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7081 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7141 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7201 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7261 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7321 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7381 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7441 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7501 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7561 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7621 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7681 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7741 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7801 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7861 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7921 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7981 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8041 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8101 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8161 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8221 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8281 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8341 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8401 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8461 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8521 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8581 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8641 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8701 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8761 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8821 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8881 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8941 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9001 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9061 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9121 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9181 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9241 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9301 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9361 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9421 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9481 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9541 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag agagaaaggc
     9601 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9661 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9721 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9781 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9841 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9901 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9961 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10021 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10081 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10141 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10201 ggaacaaaag ctggagctgc aagctt
//
