LOCUS       sequence-472694-       11336 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of structurally predicted WWE domain of TRIP12 with EGFP 
            on C terminal .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Al-Rahahleh RQ, Roos WP, Saville KM, Andrews JF, Wu Z, Koczor CA, 
            Prakash A, Sobol RW
  TITLE     Overexpression of the WWE domain of RNF146 modulates 
            poly-(ADP)-ribose dynamics at sites of DNA damage.
  JOURNAL   DNA Repair (Amst). 2025 Jun;150:103845. doi: 
            10.1016/j.dnarep.2025.103845. Epub 2025 May 21.
  PUBMED    40403420
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Repair 
            (Amst)."; date: "2025-06"; volume: "150:103845. doi"; pages: " 
            10.1016/j.dnarep.2025.103845. Epub 2025 May 21"
COMMENT     Sequence Label: pLV-EF1A-TRIP12-391-938-EGFP-Hygro
FEATURES             Location/Qualifiers
     source          1..11336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4863..4865,4866..4868,4869..5582)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4863..4865,4866..4868,4869..5579)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4908..4929)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5169..5188)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5516..5537
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5583..5607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5625..6213
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5678..5698)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6096..6107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6244..6743
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6279..6302)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6676..6695
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6760..7785
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6760..7785
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7857..8090
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8162..8283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8199..8218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8253..8272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8323..8458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8478..8497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8479..8497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8507..8524)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8507..8523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8516..8538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8665..9120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8752..8771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8962..8983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9146..9250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9251..9319,9320..10111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10282..10870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10771..10790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11024..11041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11158..11179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11194..11199,11200..11217,11218..11224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11232..11248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11237..11259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11256..11272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11256..11272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11291..11311
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11293..11311
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gaatctgaaa
     3181 gtgacgactc agagatgggg aggctgcagg ccctgctgga ggccagggga ctgcctcctc
     3241 acctgttcgg gcctctgggg cctagaatgt cccagctatt tcaccgcact atcggcagcg
     3301 gggcctccag caaggcccag cagctgctgc agggcctgca ggccagcgac gagtcccagc
     3361 agctgcaggc cgtgatcgag atgtgccagc tgctggtgat gggcaacgag gaaacactgg
     3421 gaggttttcc agtgaaatca gtggtgcctg ccctgatcac cctcctgcag atggagcaca
     3481 atttcgacat aatgaaccac gcatgcagag ccctgacata tatgatggag gccctgccca
     3541 gatcatccgc cgtggtggtg gacgccattc ccgtgtttct ggagaagctg caggtgatcc
     3601 agtgcatcga cgtggctgag caggccctga ccgccctgga gatgctgtcc agaagacact
     3661 ccaaagctat cctccaggcc ggcggcctgg ccgactgcct cctgtacctg gaattttttt
     3721 ccatcaacgc ccagcggaac gccctggcta ttgccgctaa ctgctgccag tccattacac
     3781 cagatgagtt ccatttcgtg gccgacagcc tcccactgct gactcagcgg ctgacccacc
     3841 aggacaagaa gtctgtggag agcacctgcc tgtgcttcgc ccggctggtg gacaacttcc
     3901 agcacgaaga gaacctgctg cagcaggtgg ccagcaagga cctgctgact aacgtgcagc
     3961 agctgctggt ggtgaccccc cccattctga gttctggcat gttcatcatg gtggtgagga
     4021 tgttcagcct tatgtgctct aactgcccca ctctggcagt gcagctgatg aagcagaata
     4081 tcgccgagac cctgcacttc ctgctgtgtg gcgcatccaa tggcagttgt caggagcaga
     4141 tcgacctggt gccacgctca ccccaggaac tgtacgagct gaccagcctg atttgtgaac
     4201 tgatgccctg cctgcctaag gagggcatct tcgccgttga taccatgctg aagaaaggca
     4261 atgcccagaa taccgacggg gccatctggc agtggcgtga tgatagaggc ctgtggcacc
     4321 cctataacag aatcgactcc agaatcatcg aacagatcaa cgaggacacc ggcacagcaa
     4381 gagctatcca gcggaagcct aatcccctgg ccaactccaa tacttccggt tatagcgaat
     4441 caaagaagga cgatgcccgc gcccagctga tgaaggagga ccccgagctg gccaaatcct
     4501 tcattaagac cctgttcgga gtgctgtacg aggtgtacag cagcagtgct ggccccgccg
     4561 tgaggcacaa atgtctgagg gctatcctgc gcattatcta cttcgccgat gccgagctgc
     4621 tgaaagacgt gctgaaaaac catgctgtgt cttcccacat cgcctccatg ctgagcagcc
     4681 aggacctgaa gatcgttgtg ggcgccctgc agatggccga aatcctgatg cagaagctgc
     4741 cagatatctt ctccgtgtat ttccgcaggg agggcgtgat gcatcaggtg aaacacctgg
     4801 ccgagagcga gtcctctggc gggggcggca gcgggggcgg cggctccggc ggcggcggtt
     4861 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4921 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4981 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5041 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5101 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5161 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5221 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5281 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5341 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5401 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5461 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5521 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5581 aaacccagct ttcttgtaca aagtggtgat aatcgaattc cgataatcaa cctctggatt
     5641 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5701 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5761 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5821 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5881 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5941 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6001 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     6061 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6121 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6181 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcccgcgg ttcgaattct
     6241 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     6301 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     6361 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     6421 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     6481 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     6541 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     6601 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     6661 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     6721 cttcctcatc tccgggcctt tcgacctcac gtggccacca tgaaaaagcc tgaactcacc
     6781 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     6841 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     6901 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     6961 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     7021 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     7081 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     7141 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     7201 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     7261 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     7321 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     7381 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     7441 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     7501 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     7561 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     7621 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     7681 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     7741 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagggtac ctttaagacc
     7801 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7861 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7921 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7981 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8041 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8101 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8161 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8281 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8341 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8401 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8461 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8521 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8581 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8641 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8701 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8761 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8821 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8881 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8941 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9001 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9061 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     9121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9241 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9301 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9361 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9421 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9481 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9541 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9601 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9661 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9721 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9781 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9841 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9901 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9961 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10021 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10081 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10681 aagcgccacg cttcccgaag agagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10921 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10981 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11041 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11101 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    11161 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    11221 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    11281 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagctt
//
