LOCUS       sequence-472696-       12696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green glucose sensor, moderate sensitivity, nuclear.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Lopez-Pajares V, Bhaduri A, Zhao Y, Gowrishankar G, Donohue LKH, Guo
            MG, Siprashvili Z, Miao W, Nguyen DT, Yang X, Li AM, Tung AS, 
            Shanderson RL, Winge MCG, Meservey LM, Srinivasan S, Meyers RM, 
            Guerrero A, Ji AL, Garcia OS, Tao S, Gambhir SS, Long JZ, Ye J, 
            Khavari PA
  TITLE     Glucose modulates IRF6 transcription factor dimerization to enable 
            epidermal differentiation.
  JOURNAL   Cell Stem Cell. 2025 Mar 20:S1934-5909(25)00088-8. doi: 
            10.1016/j.stem.2025.02.017.
  PUBMED    40120584
REFERENCE   2  (bases 1 to 12696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2025 Mar 20:S1934-5909(25)00088-8. doi: 
            10.1016/j.stem.2025.02.017."
COMMENT     Sequence Label: pLEX.(NLS)iGlucoSnFR.L276V.K312A
FEATURES             Location/Qualifiers
     source          1..12696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1291..2316)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1291..2316)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(2365..2694)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2379..2514
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2433..2452)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2679..2699
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2708..3136)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2818..2839)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3030..3049
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3182..3406
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3395..3412
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             3513..3746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3953..4541
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4059..4070
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4468..4488
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4548..5150)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4631..4651)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5153..5725
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5312..5331)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5541..5558
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5795..5824)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     6162..6181
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6421..6442
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6548..6569)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(7775..7822)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(7893..7917)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7914..8117)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7943..7963)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8118..8421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8526..8573
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        8565..8583
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      8587..8596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8593..8967
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9032..9149
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(9447..9491)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9676..9909
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10406..10531
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10578..11211
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11379..11513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11561..11665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11666..11734,11735..12526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11884..11903)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg ctattccttt gccctcggac gagtgctggg
     1321 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     1381 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     1441 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     1501 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     1561 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     1621 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     1681 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     1741 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     1801 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1861 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     1921 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     1981 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     2041 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     2101 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     2161 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     2221 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     2281 ctcgacagac gtcgcggtga gttcaggctt tttcatcacg tgctgatcag atccgaaaat
     2341 ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca
     2401 ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat
     2461 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     2521 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     2581 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     2641 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     2701 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2761 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2821 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2881 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2941 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3001 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3061 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3121 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     3181 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     3241 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     3301 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     3361 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     3421 cgggtttaaa caatgtcaag gcctctcact ctctgatatt catttctttg caagttataa
     3481 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     3541 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     3601 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     3661 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     3721 tcgttgggag tgaattagcc cttccagtcc cctcttttct tttaaaaagt ggctaagatc
     3781 tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac
     3841 ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct
     3901 acttgtgatt gctccatgtt tttctaggtc tcgactgcag aattaattcc aggcggggag
     3961 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
     4021 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
     4081 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac
     4141 gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
     4201 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
     4261 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
     4321 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
     4381 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
     4441 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
     4501 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgttaactca ggcaccgggc
     4561 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     4621 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     4681 accccagcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     4741 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
     4801 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     4861 gccatgcgcg agccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     4921 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     4981 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cgggtcgacg
     5041 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg
     5101 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtggccat ggtattatcg
     5161 tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt tttttaacct
     5221 cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca tacaatgggg
     5281 taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct
     5341 ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc
     5401 ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct
     5461 gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt
     5521 cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct
     5581 cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt
     5641 ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtaac
     5701 gttagggggg ggggagggag aggggcgacc ggtggccaga cgcgtcgggc cctctagact
     5761 cgagtcagct gcccacctcg ttctcgcagt agaacagatc ctcttctgag atgagttttt
     5821 gttcgtcgac ctgcagctgg cccaggcggc ccaggcccac ctgatcggca atggcctggg
     5881 cggcgttggc ggcggcctgc gggttgcggg tttgcagaaa aatttccatc acggtgccaa
     5941 actggctcat aaagctttcc ggggccacgg cgccatgcac caggctgccc acaatgcggt
     6001 tgctgcgcca atcgcgcatg gcgctctggc cataggcgtt ggggttgtta aagttgtact
     6061 ccagcttatg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     6121 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     6181 ccttgaagaa gatggtgcgc tcctggatgt agccttcggg catggcggac ttgaagaagt
     6241 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     6301 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     6361 gcttgccgta ggtggcatcg ccctcaccct cgccggacac gctgaacttg tggccgttta
     6421 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     6481 tcaccatgct ccctccggta ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     6541 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttcgaaa
     6601 gtttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     6661 gggtgttctg ctggtagtgg taggcgagct gcacgccgcc gtcctcgatg ttgtggcgga
     6721 tcttgaagtt cgccttgatg ccgttcctct gcttgtcggc catgatatag acgttgtggc
     6781 tggcgggata tttgctcgga tcgctatcca ggcgggcggc aatgctgcct gccagcgggt
     6841 taaaggtatc ctggccttct ttgctgccca ccaggcgcag ccagttaatg gcgttctggc
     6901 ggtttttggc gcctttcggc aggccaaagc tatcgctcac catcataaac acgccctggg
     6961 tgcccgggct cggggcccag gcaaaatcgg tgcccggttt cagtttcagg gtggtggtca
     7021 tatagccggc ggcccaatcg cccatcacgt taaaggcggc tttgccctgc accacgcgat
     7081 ccacggcctg ctgccagctc aggccggcgg catctttgtt ggcgcaatcc agcacgcggc
     7141 caaacacttc ccaggcgcgc acggctttcg gatcggtaaa tttcagtttg ccgttccaca
     7201 ggttgttcca atcatccggg cccagcacag ccagggccac gctttcccac agatgctgct
     7261 gggtccagtt ttcgcccagg gccagcgggg cttccaggcc tttctgtttc agggtctggc
     7321 aggtggccag aaattcatcc caggtgcgcg gcgggttcac gccccattct ttcagtttgg
     7381 ccggcagata ccacatcacg ttgctgcgat gaatgttcac cggcacgctc caaatgccgc
     7441 ctttatagct aatcagatca atcaggcctt tcggaaaggc ttgcagccag ccttcctggc
     7501 gaaacagggc gctcaggtct tccatgcggt tggccaccac ccaggtgcca atcagttcca
     7561 tgccggcatg cacctgaaag gtatccggcg gatcgccgcc cagcatgcgg gttttcagca
     7621 cggcgcgggc gttcacgccg gcgccgccgg tcacggtggc gttaatcacc tccacgcccg
     7681 gatatttctg tttatacagg cgaatcaggg cttccagggc cgggccttca tcgccggccc
     7741 accagctaaa aatttccagt ttagatctgt cacctttctt cttcttagcc tgtccagcct
     7801 tctttgtggc ggcaggtcgc ttcatggtgg atcctctagt agagtcggtg tcttctatgg
     7861 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg
     7921 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     7981 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     8041 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     8101 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     8161 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     8221 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     8281 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     8341 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     8401 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     8461 attgattact attaataact aatgcatggc ggtaatacgt ctagaacctc ggacctgcag
     8521 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gactcactat
     8581 aggagggcca ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     8641 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     8701 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     8761 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     8821 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     8881 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     8941 cacttcgtgg ccgaggagca ggactgattc cggactagag cgggcccggt actaaccaaa
     9001 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
     9061 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
     9121 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga
     9181 attggcgcac gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct
     9241 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     9301 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     9361 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     9421 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     9481 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     9541 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     9601 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     9661 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     9721 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     9781 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     9841 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     9901 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     9961 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
    10021 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg
    10081 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
    10141 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
    10201 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
    10261 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
    10321 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
    10381 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
    10441 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
    10501 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
    10561 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
    10621 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
    10681 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
    10741 gtctaaccag agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg
    10801 ctcgccactc cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc
    10861 ccttgtagca agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca
    10921 cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat
    10981 ccatcccatg caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt
    11041 ctaccttatc tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc
    11101 tgacccctgg ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga
    11161 tccacagatc aaggatatct tgtcttcttt gggagtgaat tagcccttcc aactactaag
    11221 tttgtagtac atatttaaca aatacaattt ctttaaaatg aaaataattc agaggaatca
    11281 caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta tggggtggga
    11341 agtttgggat gattttggtt agcttgagtt atccagttga tccagacatg ataagataca
    11401 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
    11461 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttgatctcc
    11521 cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    11581 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11641 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    11701 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    11761 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    11821 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    11881 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    11941 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    12001 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    12061 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    12121 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    12181 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    12241 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    12301 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    12361 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    12421 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    12481 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    12541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    12601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    12661 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
