LOCUS       sequence-472700-       13386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Green glucose sensor with mRuby2 fusion, high sensitivity, 
            cytosolic.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Lopez-Pajares V, Bhaduri A, Zhao Y, Gowrishankar G, Donohue LKH, Guo
            MG, Siprashvili Z, Miao W, Nguyen DT, Yang X, Li AM, Tung AS, 
            Shanderson RL, Winge MCG, Meservey LM, Srinivasan S, Meyers RM, 
            Guerrero A, Ji AL, Garcia OS, Tao S, Gambhir SS, Long JZ, Ye J, 
            Khavari PA
  TITLE     Glucose modulates IRF6 transcription factor dimerization to enable 
            epidermal differentiation.
  JOURNAL   Cell Stem Cell. 2025 Mar 20:S1934-5909(25)00088-8. doi: 
            10.1016/j.stem.2025.02.017.
  PUBMED    40120584
REFERENCE   2  (bases 1 to 13386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2025 Mar 20:S1934-5909(25)00088-8. doi: 
            10.1016/j.stem.2025.02.017."
COMMENT     Sequence Label: pLEX.(Cyto)iGlucoSnFR.H66A.L276V-mRuby2
FEATURES             Location/Qualifiers
     source          1..13386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1291..2316)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1291..2316)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(2365..2694)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2379..2514
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2433..2452)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2679..2699
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2708..3136)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2818..2839)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3030..3049
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3182..3406
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3395..3412
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             3513..3746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3953..4541
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4059..4070
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4468..4488
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4548..5150)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4631..4651)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5153..5725
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5312..5331)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5541..5558
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(5795..6505)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             complement(6536..6565)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     6903..6922
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7162..7183
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7289..7310)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8583..8607)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8604..8807)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8633..8653)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8808..9111
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9216..9263
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        9255..9273
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9277..9286
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9283..9657
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9722..9839
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10137..10181)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10366..10599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11096..11221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11268..11901
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    12069..12203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        12251..12355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12356..12424,12425..13216)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12574..12593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg ctattccttt gccctcggac gagtgctggg
     1321 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     1381 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     1441 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     1501 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     1561 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     1621 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     1681 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     1741 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     1801 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1861 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     1921 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     1981 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     2041 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     2101 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     2161 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     2221 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     2281 ctcgacagac gtcgcggtga gttcaggctt tttcatcacg tgctgatcag atccgaaaat
     2341 ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca
     2401 ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat
     2461 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     2521 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     2581 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     2641 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     2701 cagaattaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2761 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2821 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2881 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2941 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3001 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3061 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3121 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     3181 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     3241 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     3301 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     3361 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     3421 cgggtttaaa caatgtcaag gcctctcact ctctgatatt catttctttg caagttataa
     3481 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     3541 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     3601 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     3661 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     3721 tcgttgggag tgaattagcc cttccagtcc cctcttttct tttaaaaagt ggctaagatc
     3781 tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac
     3841 ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct
     3901 acttgtgatt gctccatgtt tttctaggtc tcgactgcag aattaattcc aggcggggag
     3961 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
     4021 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
     4081 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac
     4141 gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
     4201 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
     4261 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
     4321 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
     4381 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
     4441 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
     4501 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgttaactca ggcaccgggc
     4561 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     4621 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     4681 accccagcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     4741 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
     4801 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     4861 gccatgcgcg agccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     4921 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     4981 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cgggtcgacg
     5041 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg
     5101 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtggccat ggtattatcg
     5161 tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt tttttaacct
     5221 cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca tacaatgggg
     5281 taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct
     5341 ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc
     5401 ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct
     5461 gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt
     5521 cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct
     5581 cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt
     5641 ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtaac
     5701 gttagggggg ggggagggag aggggcgacc ggtggccaga cgcgtcgggc cctctagact
     5761 cgagtcagct gcccacctcg ttctcgcagt agaacttgta cagctcgtcc atcccaccac
     5821 caagcccggc gaacttggca actgcgtgtt cgcgttgtac tacgaacatt tcattgtcac
     5881 tttcctctaa cctttccagg cggtgatcaa cggcatggat accgggcatc ttgatgttcc
     5941 cgacggtctt ttttgacctg taagttgtta cgaaagagca agacagatgg ccaccaccat
     6001 caactttcag tgccatatga gtgtatcccc tcagaccacc atctgctgga tacatcatct
     6061 ctgtattagg ctcccaaccc ttggtcttct tctgcatcac gggaccattg gagggaaagt
     6121 ttacccctct gacttggacg tggtaaacga gacagccatc ctcaaggctg gtgtcctgca
     6181 tgacggtgac gactccacca tcttcgtatc tcgtaactct ttcccaagta aaaccctcag
     6241 gaaaggactg tttaaagaaa tcaggaatgc ctttcgggta cttgataaaa gtacggctgc
     6301 catacatgaa cgacgtggca agaatgtcaa aggcaaatgg caggggtcct ccctcgatga
     6361 ctttgatcct catggtttga gttcccatgt acggattgcc ttctccttca cctgtgcatt
     6421 tgaattggtg gccgttgacc gaaccttcca tgaccacctt catacgcata ttttccttga
     6481 tcagctcttc gcccttagac accatacgct cctgcgtgtc ctggcccaca gcattcagat
     6541 cctcttctga gatgagtttt tgttcgtcga cctgcagctg gcccaggcgg cccaggccca
     6601 cctgatcggc aatggcctgg gcggcgttgg cggcggcctg cgggttgcgg gtttgcagaa
     6661 aaatttccat cacggtgcca aactggctca taaagctttc cggggccacg gcgccatgca
     6721 ccaggctgcc cacaatgcgg ttgctgcgcc aatcgcgcat ggcgctctgg ccataggcgt
     6781 tggggttgtt aaagttgtac tccagcttat gccccaggat gttgccgtcc tccttgaagt
     6841 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     6901 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggatg tagccttcgg
     6961 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     7021 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     7081 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcaccc tcgccggaca
     7141 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     7201 tgaacagctc ctcgcccttg ctcaccatgc tccctccggt accgcccttg tacagctcgt
     7261 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     7321 tctcgttggg gtctttcgaa agtttggact gggtgctcag gtagtggttg tcgggcagca
     7381 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtaggcgagc tgcacgccgc
     7441 cgtcctcgat gttgtggcgg atcttgaagt tcgccttgat gccgttcctc tgcttgtcgg
     7501 ccatgatata gacgttgtgg ctggcgggat atttgctcgg atcgctatcc aggcgggcgg
     7561 caatgctgcc tttcagcggg ttaaaggtat cctggccttc tttgctgccc accaggcgca
     7621 gccagttaat ggcgttctgg cggtttttgg cgcctttcgg caggccaaag ctatcgctca
     7681 ccatcataaa cacgccctgg gtgcccgggc tcggggccca ggcaaaatcg gtgcccggtt
     7741 tcagtttcag ggtggtggtc atatagccgg cggcccaatc gcccatcacg ttaaaggcgg
     7801 ctttgccctg caccacgcga tccacggcct gctgccagct caggccggcg gcatctttgt
     7861 tggcgcaatc cagcacgcgg ccaaacactt cccaggcgcg cacggctttc ggatcggtaa
     7921 atttcagttt gccgttccac aggttgttcc aatcatccgg gcccagcaca gccagggcca
     7981 cgctttccca cagatgctgc tgggtccagt tttcgcccag ggccagcggg gcttccaggc
     8041 ctttctgttt cagggtctgg caggtggcca gaaattcatc ccaggtgcgc ggcgggttca
     8101 cgccccattc tttcagtttg gccggcagat accacatcac gttgctgcga tgaatgttca
     8161 ccggcacgct ccaaatgccg cctttatagc taatcagatc aatcaggcct ttcggaaagg
     8221 cttgcagcca gccttcctgg cgaaacaggg cgctcaggtc ttccatgcgg ttggccacca
     8281 cccaggtgcc aatcagttcc atgccggctg ccacctgaaa ggtatccggc ggatcgccgc
     8341 ccagcatgcg ggttttcagc acggcgcggg cgttcacgcc ggcgccgccg gtcacggtgg
     8401 cgttaatcac ctccacgccc ggatatttct gtttatacag gcgaatcagg gcttccaggg
     8461 ccgggccttc atcgccggcc caccagctaa aaatttccag tttagatctg tcacccatgg
     8521 atcctctagt agagtcggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag
     8581 gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta
     8641 ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt
     8701 tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag
     8761 tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg
     8821 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     8881 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     8941 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     9001 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     9061 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     9121 atatatgggc tatgaactaa tgaccccgta attgattact attaataact aatgcatggc
     9181 ggtaatacgt ctagaacctc ggacctgcag cacgtgttga caattaatca tcggcatagt
     9241 atatcggcat agtataatac gactcactat aggagggcca ccatggccaa gttgaccagt
     9301 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     9361 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     9421 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     9481 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     9541 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     9601 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgattc
     9661 cggactagag cgggcccggt actaaccaaa ctggatctct gctgtccctg taataaaccc
     9721 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     9781 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
     9841 tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga tccgttcact
     9901 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     9961 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
    10021 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
    10081 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
    10141 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
    10201 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
    10261 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
    10321 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
    10381 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
    10441 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
    10501 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
    10561 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
    10621 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
    10681 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
    10741 aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
    10801 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
    10861 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
    10921 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
    10981 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
    11041 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
    11101 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
    11161 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
    11221 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
    11281 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
    11341 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
    11401 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa
    11461 aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac
    11521 gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat cagcagttct
    11581 tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc
    11641 tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca
    11701 agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact ggtactagct
    11761 tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg tagttctgct
    11821 aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcttt
    11881 gggagtgaat tagcccttcc aactactaag tttgtagtac atatttaaca aatacaattt
    11941 ctttaaaatg aaaataattc agaggaatca caggtttaga gtaaatgaaa ccacaggtaa
    12001 ttggcagtgg taatagggta tggggtggga agtttgggat gattttggtt agcttgagtt
    12061 atccagttga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
    12121 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
    12181 ttataagctg caataaacaa gttgatctcc cgatccgtcg acgtcaggtg gcacttttcg
    12241 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    12301 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    12361 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    12421 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12481 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    12541 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    12601 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12661 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    12721 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    12781 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12841 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12901 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12961 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    13021 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    13081 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    13141 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    13201 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    13261 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    13321 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    13381 atcttc
//
