LOCUS       sequence-472722-        3146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries the X. dendrorhous SAM promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdSAM
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     468..487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(706..810)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     878..896
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(934..956)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1056..1075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1893..1904
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(2232..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2312..2331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2991..3146,1..636,637..705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
       61 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      841 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      901 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      961 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1021 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactggg
     1081 tctcagtgct tcaaagggta aaatatcacg tcgtaggaaa gcctaacgaa catcacagac
     1141 agccgtcacg gttgtatctt ggttcatacc tcacaccttc tggtgatcca tcagccaagc
     1201 cccaaacttt tctttccgtg cggacatgga tatagaaaga tcaactaaaa ttcatatgtt
     1261 ctttcaaccc atacaccaaa tcgagatacg acgtcaaaat gtgtcgtgat aataacaata
     1321 aaaagatgaa agctaaaaaa cccaagttaa actctgtccc aagatctgga gcttgcaacg
     1381 aaaggaagct ggtccagtat catcgtcgag gagatcatga cagtattggc ctcactgatt
     1441 gggagagtac agtctatgat agctagttcg cagggatcat gtttgtttat gaatcacaat
     1501 atgcgaattt agcgcaaagt cctataagtg gtaatggcaa gaatgtttcg aagaaggagt
     1561 atatgtcgac aagacaaaga gaagatagga aaggactcaa gacgaggaag gctctgatct
     1621 tgatatccaa gaatgacatg aaatgatagg gtcgataaga agatagaggg aacccgtccg
     1681 gtaggtacga tcagaagaac tatctgggag gttctggaat gagaggatgg tgatgcagtg
     1741 tatctaatac aagcatcaaa taacgacagt atctgataag tttacccatc ccacaggcgt
     1801 cgccccggtt gacgcagatc agtcagaggg agagacactg catatcggag ggaccacagg
     1861 agacacggga cagagccgcg cgagatcatg aaatagaggg acgatcagcg attgtctcgg
     1921 ctgagggtta ctatcaccaa gcctaaggct tatcatagat cggtgcacgc gacggtgaaa
     1981 ttccgatgga aatcccatta atcggtcatc cagatcgagt atgtattccc atcagatcac
     2041 tagtttcctc tgagtaatcg tacagtgtcc tggttctctt gctcttcatc aatgagagac
     2101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagcccaac ccggtaagac acgacttatc gccactggca
     2581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctgg
     3061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3121 atgataccgc gagagccacg ctcacc
//
