LOCUS       sequence-472818-       11675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N terminal GFP tagged Myo5B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Zhao G, Liu S, Arun S, Renda F, Khodjakov A, Pellman D
  TITLE     A tubule-sheet continuum model for the mechanism of nuclear envelope
            assembly.
  JOURNAL   Dev Cell. 2023 May 22;58(10):847-865.e10. doi: 
            10.1016/j.devcel.2023.04.003. Epub 2023 Apr 24.
  PUBMED    37098350
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.04.003"; journalName: "Dev Cell"; date: 
            "2023-05-22- 22"; volume: "58"; issue: "10"; pages: "847-865.e10"
COMMENT     Sequence Label: eGFP-MYO5B
FEATURES             Location/Qualifiers
     source          1..11675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2264..2283)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8873..9586)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(join(8873..9583,9584..9586))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8915..8936)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9264..9283
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9523..9544
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9627..9646)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9628..9646)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9691..9894)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9720..9740)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9895..10274
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10446..10465
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10540..10644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10645..10713,10714..11505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10863..10882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcgggtttaa acgggccctc tagactcgag cggccgccac tgtgctggat
     3301 atctgcagaa ttcggatcct cagacttcat tgaggaattc cagattgaga cacgctggga
     3361 tgtggattga gtccatggtt agagaagatg gattaaatgg aaacaaaaca ggaaacatgt
     3421 gcttggcatc taatagcagt tgctgagggt cattccgctc ttgtagttgt gcctggattg
     3481 ttcgtataaa ggccactgtt acccgttctt caaattcatt caggggagta taaaggttta
     3541 aaattttgac aatctgctgg gtgctgaggg aggtacacag ggagcagata gcctctgcgt
     3601 cctcctgggt tttcttcttt aattgcagga gctgggctgc ttggatcaga ggttccatgg
     3661 tctgaactgc tccactctgg tgaaggtttc ttccccgaag ccactcctca agctgactta
     3721 tattgtacct gagttgcatg cctgtgctcc aagagcagac gtccttccgc aagagcaggt
     3781 tgttaagagt cactgcgttg atcatgtaga agagctgttt gaatacctgc aggatgatct
     3841 cagggtccaa gccctggtca cacatgactg tatgaaaggc attcatctgg cggatgatag
     3901 cttccaggca gtatgagtta tccccatctg ccatgctgga ggagcgcttc cggtagccgg
     3961 tgggcttcac accagataga ccctgaatgc tctcattttc caacatggca gaaactatca
     4021 tcggctgtaa cacgccctcg gcaattttaa tgagctgctg gtagatctga atggaaaggt
     4081 cactcagcac ctgacggtat tcggtgaggt caaaattctt aagacagtgt tcattctgct
     4141 ttgcagtgtt ctgagtcatg aagccctcat ccccgctgta ctgcttcaga cagtgaagaa
     4201 ggcggcaggt gttggataac cagaatgacg tcatctcaaa gtcatcattg tgctttttca
     4261 ggactttctt aatgccgttg atggtggagg tcagcaggga gtgcaccttg agatcgtcgt
     4321 tggtgtagtc cgcgtgccgg atgcacatgt agaggatgta ggcggggaga cagggcactg
     4381 tgcccgacag catctggggc ttcaagtctg tcaccaggtt ccggatgagg agggcctcgt
     4441 cctctttgtg gtactccagc atgccctgga aatccttctc tttccgctgg accgtgacct
     4501 gcctgttgag ctcatggcgc ttcctctcac tctgggccaa tgcctgggca gcttctaggt
     4561 cctgggcttt cttcatgtaa atcttcagtt gctttttgag cttcctctca ttcttttcca
     4621 gcttttctac cagttcttta aggtccagat tctcgttggt cagccgggat atttcctgct
     4681 gaacgccgaa ttccacctgg gcctctgggg agagcagtag cgtctggcag aaggtctgct
     4741 gctgtttgtc catctcctcc ttcagggcct cgagctgagc cttgagatgc tccacctcct
     4801 cctcatgctc caggctctgg gcctgcagct gagcctccag cagcctggca acttgcttta
     4861 ggccttggta ggccaagccg agttctccat cttcatttaa atatccccaa tcctcagtct
     4921 tgctgtttgt ctggcagacc ccgtgatagg cctcaatggc atcctcctgg tcaacatgct
     4981 tttcactgtt aggccaactt gatctggcat taatgttcgg ctccgcgttc ctgccggcga
     5041 gtcgccgctg gtcggcgctc acgatctggg tcctgaggat gagcacctcc tccttgcgca
     5101 cctcgagctc ctcgtgggcc agcttgagct ggttcagcag gaggctgtag ctatctgggg
     5161 agccgtggct ggagttattc tgcgtggctt ggtcggccac ggctttcctc agctcattca
     5221 ggtcattctt cagctttttg ttctctgact ccagctcttg cctcttcaga ctattgtagg
     5281 ccagatctgc attcgggtcc aaatctatgt cagtctgtgg tggttccgcc tggactttct
     5341 tgctgtcctg ctgttctctc ttctccagct gcacttgcag ctttttcctc tcctgctcca
     5401 gctcccgtac tctcttctgc agcttcagga agaccgtcat gtccatggct gccttctcca
     5461 ggccaatttc ctccacctgc tggagggcat cctcagtgtc tccgatctca gatgtggaga
     5521 tggaggggta attggagtca gattctaagc tactttggtt tgatgggttc cgcctatgac
     5581 ctggagtttg ctttatgatg gtcatttcat cccgaaggtt gtcgtatctc tgctccaact
     5641 gtgaatattc cttcacaagg ttctggtacc gggatcgctc ctcctccagt tctttcttca
     5701 tgagattttc cttcacagag ttctgggcaa attcatcttt agactggcac aggatttggt
     5761 tgttgagctg ttctttctca tctttcaaga gagcattttc ttgctccagg tctgcaactc
     5821 gcttcctcag ctcatctttc tccctgctgt gggcgtcctc caagatcttg cgctccgagt
     5881 gggccctctg cagctctgtg cgcaggctct ccacctcctc ctgcagcctg aggctggtgt
     5941 cctcacctgg gctctgctgg tagtgcacca gctccttctt cagccgctct acctccatgg
     6001 tgtatgttga ggtggtcacg gacaactgct ctgaaagtgt cttgaactct ttgttctgct
     6061 catcgatctt ccgctgcagc tggaccacct tgttctccat gcccacgttg agacgtttca
     6121 gatgctctgc tgagcgggcc tcaatcctga gggccttcag ctcccgcctg gccttgagca
     6181 tccggaaggc acactggatg acaatggctg catcccgcag ccgctggaag tgcctgcgtg
     6241 ccatccagcc ccgcacgtgc ttctggatgg tggtggcctt gtgctccatg aggacctggc
     6301 ggtaggttct ccgcacaaac atggcccggg tgaaggcctg gataacaacg gcagctctgc
     6361 ggaccctctg gtaggcctgg cgggccctct gcatgcggta atgtttctgg agcaccacag
     6421 ccgctctgat cctccgcagg tgctcagcca gcctgcgggc caggtgtccc cggcagtacc
     6481 tctgcagggt taaggtagcc cccttcagcc tgtgatattt caccttctgc agccatcccc
     6541 ggacagtttt ctggatcatg atggtggctg tccggaactt gtcagcccgc agcttctcca
     6601 ggtaggccac ctggcctgct cgaaagaaga tcttggtgcg gccaaactgg aacttgtcgg
     6661 ggtccttgat gaggttctcc aggacagacc tgcagatggc ctttttgtct gtgttggcga
     6721 gctctctctt cttgaccagc acccgatacc ggttgaaaaa gtcatggtag gcccacctgg
     6781 atgggtagcc agctgcactg attcgaatcg tctccaacac cccgcaggct ctgagttgct
     6841 gcactgctct ctttgggtca aagtgaaagg ggagcttctc atcgttgggc ttgatgcagc
     6901 ggacatagtg aggtgtcgtg gcattcaggg tctccatgag cagatgcagg gaggtacgga
     6961 actggtggcc aacggttttc ttgtgctcct tgttggagac tttcatgggg ggtctggcag
     7021 aacggacgct gatcttcgaa gatgacccct tcccaggggt ggtggcagga acagggtcct
     7081 tgtcatcatg aaacaagtca gccactagtg ggaacttgct ggccttcagg atattgatct
     7141 gctcttcata caccgtgtct ctgtttttct ccagaaaacc atcagagagg tactccacct
     7201 tgtctgcaaa gtggacgatg atgaaggccg tgttggacat gcggggcttc tggaagtgct
     7261 ggctgctgga gtgccggtca tagagcttct gagcccagtt ctggtcagtt cctttgggga
     7321 ccttacattc ttcatccaac aggtccaaga tacccagctt ggcttcaatg aggtcgatac
     7381 aaggttggtt atcataaaaa tcaatcaggg tccaagggat ctgttccttc atgtattctt
     7441 cttgctccag tttgaaaaca tgcgagttga actgctgctg gagcttttca tttgcatagt
     7501 tgatacagaa ctgctcaaag ctgtttacct caaatgtctc aaacccatag atgtccagga
     7561 ccccgatgaa ggagtgctgc ttgagggagg tgtgcagggc cttgttgatg tgctccacaa
     7621 tccagccgaa caactgggca tagatgtgct tcgccagggc gttgcgcgca ttgatcacct
     7681 gctgcaggga catggtcttg acgtaggtct ccgaggtggt gaccagcttg cgatgacaca
     7741 gccagtgctc catctgactg tgctccaccc ctagcagtcg gcagaagttg cttaggtata
     7801 catcctgggg tgatatacta caggaatcac catcacgctc agcctgaatc gccacacttc
     7861 caaggtgcaa gatagaagca attatcttaa aaatgctcat ctgatgggac tctttcactc
     7921 cgaggagtgt gaaggcttgt cgagtcttct caaagtcctc agcatcgtcc acaccctcga
     7981 tggaagtgtc tcctccctgt gatgtataga aaaagtcctc tgcacttgtt agtgcaagct
     8041 ctttaaattc tggaagaccg gcagcagcac agagctggta aaagatgtgg taattcctct
     8101 catcatctgc ctggaagacc actctggact tctccaagag gtaagtcctc atgttggccc
     8161 cgatgatgtg gtaccttttg tcaaagccaa tctggatgta cttgccaaaa cggctgctgt
     8221 tgtcattgcg ggtggtcttg gcatttccaa tggcctccat gatgggactg gatgccagca
     8281 ccttctcttc gatgttggtt tcactggccg agccaccaac ggtggcgaaa tagcgcatgg
     8341 catacttggc tgataccgtc ttcccggctc cagactcccc actgactatg atggactgat
     8401 tcttctcatc tctggccatc tgcttgtagg cttcttctgc cacagcaaag atgtgggggt
     8461 ccatgtctcc catgttttgg ccactgtagg tatagatgac atcttgtcca tagattggca
     8521 actgttcata aggattaatg gcaacaagta cgataccaca gtaagtgtag atatggttgg
     8581 actccaggaa acggaccttc aaattatgca aaactgcagg ctcatgaaga tagctaaggg
     8641 cagtcaggtc attttctccc accaagatat ctggattccg taagaagggc agctggttgc
     8701 gttgtacatc aattgggtat tccagaatcg tttcatcctc cagtctgagc tgtaggctct
     8761 tgtctccttc tttgtagtcc ttggttaact cagctgagcg ccatacctca tcagggtcag
     8821 ggatccagac ccttgtgcac tggctgtaga gctcgcccac cgacatggta cccttgtaca
     8881 gctcgtccat gccgtgagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     8941 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
     9001 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     9061 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     9121 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     9181 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     9241 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     9301 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     9361 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     9421 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     9481 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     9541 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcatggtg gcaagcttaa
     9601 gtttaaacgc tagccagctt gggtctccct atagtgagtc gtattaattt cgataagcca
     9661 gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac
     9721 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
     9781 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
     9841 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
     9901 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
     9961 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
    10021 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
    10081 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
    10141 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
    10201 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
    10261 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct
    10321 aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat
    10381 tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa
    10441 ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc
    10501 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    10561 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    10621 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    10681 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10741 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10801 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    10861 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    10921 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10981 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    11041 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    11101 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    11161 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    11221 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    11281 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    11341 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    11401 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    11461 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    11521 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    11581 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    11641 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
