LOCUS       sequence-472819-       11357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N terminal GFP tagged Myo5C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Zhao G, Liu S, Arun S, Renda F, Khodjakov A, Pellman D
  TITLE     A tubule-sheet continuum model for the mechanism of nuclear envelope
            assembly.
  JOURNAL   Dev Cell. 2023 May 22;58(10):847-865.e10. doi: 
            10.1016/j.devcel.2023.04.003. Epub 2023 Apr 24.
  PUBMED    37098350
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.04.003"; journalName: "Dev Cell"; date: 
            "2023-05-22- 22"; volume: "58"; issue: "10"; pages: "847-865.e10"
COMMENT     Sequence Label: eGFP-MYO5C
FEATURES             Location/Qualifiers
     source          1..11357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2264..2283)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8555..9268)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(join(8555..9265,9266..9268))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8597..8618)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8946..8965
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9205..9226
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9309..9328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9310..9328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9373..9576)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9402..9422)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9577..9956
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10128..10147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10222..10326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10327..10395,10396..11187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10545..10564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcgggtttaa acgggccctc tagactcgag cggccgccac tgtgctggat
     3301 atctgcagaa ttcggatccc tataacctat tcagaaagcc tagcttgaaa ctgctgggga
     3361 tctgaatcat ttccagagca tgtggagagg gggtaaaagg aaatgtgact tgaaagagat
     3421 atttggtatc caacatcagc tgtgatgaat cctcccggct atttaggaga gcctgtactt
     3481 tgcgaacaaa ggatggagtc actctcttct caaagtcatc tataggtgtg tatgaattaa
     3541 ggatctttat gatctgcaca gcagacagtg aggtgcagcg ttcgtagatc tccttggcat
     3601 cactgtctgt ggtcttcttg acctgaagca accaggctgc ctgagagagg ggctccaaag
     3661 tttcctttgc taagctgttc tgcaagttct tatctttaag ccattcttct aagtagctga
     3721 tattgcacct gatctgcatc ccttttctgc aggagcacat gtccttgcgc aggaagaggc
     3781 tgttcagcgt gaccgccccg atcaagaaga agagctgctt caccgcctgc ctcacaagct
     3841 cggggtccag gccgttctgg cacatggtgg tgtaaaagta gctcagctgt tgcaggacgg
     3901 aggtcatggt gtagccgtcc gtgtcgtcta tgctagagga gcgcttccgg aagcctgtgg
     3961 gcttcaggcc ggaaatgccc tgcaggctct catactccag cattcccgga actattatcg
     4021 gttggatatt cttttccatt atgataataa attgatgata tattcgtata gccacatcac
     4081 tgagaatctg tctgtattct gaaaggtcaa aattgttcaa gcaattctta ttctgctgtg
     4141 gactattatg cttcatgaat tcctcttctc cgctgtactg cttcaggcaa ttgagaaaat
     4201 gacaagtgtt ggaaagccaa aaggacagca tttcaaagtc ttctaaatgt tccttaacca
     4261 cctgcttgat gccattaatg gtgctgttca tgagggactt cagcatgttg gcatcattca
     4321 gagagtctgc gtagcgcaca cacatgaaca ggatatgagc cggcagcccg gggatcatgt
     4381 tcaccaccac gccacggggc ttcaagtcaa gaatgaggtt ctgaatgagc ttggcctcgt
     4441 cttctctctt gtattgcagc attccaaggt actccttggg tcctgaggac gagtgcacat
     4501 cattggcttt tccaattgtc ttgcttagtg tcttgacttg atcttgtagc tttttaatca
     4561 ctctgtcttt catgtctaat tcttcttcaa gatccctgtt ttccaaagtg agccgggatg
     4621 cttcctgccg gaagttacac ttgacttcac tttcagtttc aaattgtttc ttcaagtggt
     4681 cactggcctc ctgcatttct tgaatcttat caatcagctt ctccttctct ttggtatgta
     4741 tttcattttg ggcttccaag gcctttcttt gtgttccttc ctcctcttga ctgcgatgta
     4801 actgattaga caattcttct agttttcctt tcattttctc agcttgttca ttcaggcgga
     4861 tttcaagatc ttgcttctgt ttctccaatt ctgaaatttg ctgtttaaag tctgggatca
     4921 tcatgttctc tgatgttagc ctggtaactt catgacggat gctttcattg atgtcatttt
     4981 cttctctgaa taacttctgc aggtggttga tttcttgact gagatgcacc actttgaagt
     5041 tcaaagcttc aatctccttt tcatagcaat ccttctgaga ctggaaatgg ctctccaaaa
     5101 cacgtgttgc tttctttagt ccttcataag caaaccaaag ttctccatcc tcatttaaat
     5161 gttccagatc ttccacagag agcctgcttc ttacatcttc aatgtcatag ctttcgagaa
     5221 gttgtttggt gatctctgac atcttttctc tcatttcccg tttttgtgac tgcacatgtt
     5281 tctccacatc tatcttctgt gcctgaagta gttctatctc tttttcgaac tcagagattg
     5341 tcttgacctg cttgctcagg cgggccactt ccgccttcaa gccatcagaa gtgacgtgct
     5401 ccccctccac caggtgttgg agttgcatct tctcatcctt gagagcttta atttcttctt
     5461 tcaaagactg aatctgcttc tcatagtctt gtgttttcag ttcaaaactt ttttcaagaa
     5521 gcattctttg ccgttcttcc ttttgtacat catcaaagag ctgcttggtg aggttgtcca
     5581 ttttttcttt taactcttca gtcttctctt gaagcttcag ctgtatttgt tctttctgtg
     5641 tttccagttc tgaattatgc ttctgaagct ttgccaattt ctcttccaca gcatccctgt
     5701 atctcttccc cttctcctcg taatttcgcc tgtgagtggc tgctttttct agttctgctt
     5761 ccagcttctg aatcttttcc acatccccag ctcgaagagc agccaggcta gtcagcttct
     5821 ccaccagccc atggttttct ttgttctgat cttccaactt tttctgcaaa cgctggaccc
     5881 tgtaagtaag ctgaatatta agcacgaatc gtcggatact ctggaatctg cgtctggcca
     5941 gccacgcccg tgcgtatttc tgtaggatca cagccttatg ttcctccagc atctttcgat
     6001 acctcctcct tgccaggaat cctcggctgt aggcctgcat tgtgatggtg gccatgcgaa
     6061 tcaactgata caggctgcga acaagatacc cgcggcagtg cttctgaatg attatggctg
     6121 cccaagcttc ttttaaggcc actgcagtaa tagctttcct cacagtttgc tgaccccgga
     6181 agtactgctg gattatcagg gcggctcgtc tctctcggag gaattttttc ctctggagcc
     6241 agccacgcat gtgcttttgt accataacac aactctgcct cagtttatcc aatcgaagtt
     6301 tctctaaata agccacttgt cctgctctga agaaaatttt ggttttacca aactggtact
     6361 gattagaatc ctggatgagt ctgtgtaaaa ccaccttgca cacctccttt ttatcgctga
     6421 aggaaagctc ttgcttggtc atgagaatgc cgtagcgact gtagaactcg atgtatgtcc
     6481 acctggaagg gtagctctgt gcactaatgc gaatcgtttc taaaacgccg caggctcgca
     6541 gctgctgaac aattcttttg gagtcaaatt caaagggtaa cttctcatca tttggcttga
     6601 tgcatcgaac gtagtggggc gtcgtcgcat tgagggtctc catgagcaag tacagagagc
     6661 tgcggaactt gctcccaact gtggtccgga aatgcttgct gtttggcttg atgacttgct
     6721 ttgcagattt aactgtaatc attgaaccaa aaggagaagg aggagttgga ttttcttgaa
     6781 aaaagttggc acagagatga aacttgcttg ctctcaggat ttcaaccagc atgtcataga
     6841 cggtgtctct gtttttctcc aggaaacctt cacatttata ctctacctta tcagcaaagt
     6901 gctggatgac aaaggatgtg tttgacattc taggcttttc aaacaaaggg ttcctgttga
     6961 caaaattatt atacagcttt tgaagccagt tttcatcagt tccatgtggt aacaaacatt
     7021 cttcatccag taactccaga attcccattt ttgcttcaat caggtcaata actggttgat
     7081 tgtcataaaa atctatcagc gtccaaggta tatcttcctt catgtattct tcttgttcca
     7141 gtttgaagac atgcatgtta aactgttgtt gcagtttttc attagcgtaa ttgatgcaaa
     7201 attgttcaaa gctgttcaca tcaaaggttt caaaaccata aatgtccaaa acaccaataa
     7261 aagtgtgctg cttgcctgaa aactgcaacg cttggttaat tctctccaca atgaagtcga
     7321 acaggtgagc atagatcttt ttggccagtg catccctggc gttgacagcc tgaggcctgg
     7381 tcatgggttt taccaccgtc tcagagcttg tgacgatttt gcgattgcac agccactgag
     7441 caactctgcc actctccagg cccaggagct cacagaacac cttcaggtga ctgtcatcct
     7501 cactaactga ggacctctcg ttgcccaccg cggtgatctg cacattgccc agatgtagga
     7561 tggctgccag gattttaaaa acgtccatct gaaaatcctc cttgaaaccc agaagcgtga
     7621 aggtcttttg agtctctacc atttcagctc gatcattcac accctcaatg acagtattgc
     7681 ctcccattct tgtataatta aattcttcgg cactccccaa tttaagatgt ttaaattccg
     7741 actgctgtgc agatgcacaa agctgataga aaatgtggta atttcgttca ttttccgatt
     7801 gaaagacaac tctggatttc tccaggaggt aagtgctcat gttggctcct ataatttgat
     7861 tttgttcatc aaaactgatt tctgtgtatt tcccaaaccg actactattg tcattgcggg
     7921 tggtcttggc atttccaacg gcctcggtga tgggattgga tgccaggacc ttgtcttcca
     7981 cgtgagcgtt gctgcccgat ttgctgacgg tggcaaagta cctcatggca tagcgagccg
     8041 acactgtctt tccagcacct gactccccac ttacaattat ggactggttt ctgttgtttc
     8101 tggccatctg cttgtatgcc tcttctgcca cggcaaatat gtgtgggtcc atatcgccca
     8161 tgttctgccc gctgtaggcg tggatgatgg catctccgta tattggcaac tgcttgtaag
     8221 gattcatggc caccaaaatg attccactgt aggtgtaaat gagtttggat tctgcaaagc
     8281 ggattctgag gttgtggagc accgcgggct cgtgaagata gctgagagcc gtgaggtcat
     8341 tctcgcccac gaggatgtca ggattccgaa gtggaggcag agattctgga ttgacagaat
     8401 aatccagctc cgttccatcc tccagcagga gtcgcaggac cttgtcacca actctgtagt
     8461 ccttggctat ttcagcagac ttccaaactt cttcaggatc gggaatccag accctgttgt
     8521 actgcgtgta cagctcggcc accgccatgg tacccttgta cagctcgtcc atgccgtgag
     8581 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     8641 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     8701 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     8761 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     8821 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     8881 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     8941 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     9001 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     9061 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     9121 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     9181 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     9241 cggtgaacag ctcctcgccc ttgctcatgg tggcaagctt aagtttaaac gctagccagc
     9301 ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct
     9361 agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     9421 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     9481 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     9541 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     9601 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     9661 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     9721 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     9781 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     9841 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     9901 atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg
     9961 cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt
    10021 catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg cgcactactc
    10081 agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg catcagagca
    10141 gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc gacgtcaggt
    10201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    11041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    11101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    11161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    11221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11341 aagatcaaag gatcttc
//
