LOCUS       sequence-472829-       10807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of ScVPS30 and ZZ-ScVPS38.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO734
FEATURES             Location/Qualifiers
     source          1..10807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2069..2186
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    4531..4648
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             4985..5005
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5021..5194
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5195..5368
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             5378..5398
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5429..5449
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5471..5491
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(7205..7225)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7205..7223)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7244..7260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7244..7260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7257..7279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7268..7284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7292..7298,7299..7316,7317..7322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7337..7358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7475..7492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7646..8234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7726..7745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8405..9196,9197..9265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9028..9047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9266..9370)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9397..10739
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10360..10407)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10780..10798
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgc tatgacatga ttacgaattg gcgcgccggg ccgcggatct
     2041 ttgcaaagat ccaaaaaaag catgcgtacg gattagaagc cgccgagcgg gtgacagccc
     2101 tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc ggtcgcgttc ctgaaacgca
     2161 gatgtgcctc gcgccgcact gctccgaaca ataaagattc tacaatacta gcttttatgg
     2221 ttatgaagag gaaaattggc agtaacctgg ccccacaaac ctcaaatgaa cgaatcaaat
     2281 taacaaccag atatctcgac tgaaaaaaaa ggtttaaacc agttccctga aattattccc
     2341 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
     2401 tcttaaactt cttgaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa
     2461 gaacttagtt tcgaataaac acacataaat aaaccatggg catgaagtgc caaacatgtc
     2521 acctgccgct gcaactggac ccatctttag aaggtcttag tttaacccaa agaaatttgc
     2581 tactttcaaa taattcaatt atcactgcaa cgaatgaaaa tgtcatcagc aacaagggca
     2641 tcgaagccgc agataattgt gggcctcaaa taccgaagga gaggctgagg cgattagggg
     2701 agattcagaa cataaaagac ttgaatttaa tagatgacaa attgatcact gattcgtttg
     2761 tttttcttaa tcacgatgat gatgataatg ctaatattac gagtaatagc agagaggacc
     2821 agcgttatgg aaatgccaac ggaaatgata ataaaaaagc taatagtgat acttccgatg
     2881 gaaccagtac ctttcgagat catgacgaag aggaacaaga agctacggat gaagacgaaa
     2941 accaacaaat acaattgaat tccaagactt tgtccacaca agtcaacgca atgactaatg
     3001 tatttaacat tctatcttct caaacaaaca tagactttcc tatctgtcag gactgctgta
     3061 acatcttgat aaacagacta aagagtgagt atgatgatgc aataaaagag agagatactt
     3121 acgctcaatt tttatcgaag ttagagagtc aaaacaaaga aatatcagaa agcaataaag
     3181 aaaagcagta ttcccataat ctttcggaaa aagaaaattt gaaaaaggaa gaggaaagac
     3241 tcttggatca attattacgt ttggaaatga cagatgatga cttagatgga gaactagttc
     3301 gtttacaaga aaagaaagtt caactggaaa atgagaaact ccaaaaactg agtgatcaga
     3361 atttaatgga tttaaataac attcaattca acaaaaattt acagtcatta aaactgcaat
     3421 acgaattatc cttgaatcaa ttagacaaat tgaggaaaat caatattttc aatgctacat
     3481 tcaaaatctc acatagcggc ccctttgcca ctataaatgg attgagatta ggcagtatac
     3541 cagaatcagt ggttccttgg aaggaaatca atgcagcact gggccaattg attcttttac
     3601 tggctaccat aaacaaaaac ctgaagataa atttagtgga ctacgaatta caaccgatgg
     3661 gttcattttc caaaatcaag aaaagaatgg ttaacagtgt cgaatataat aattccacta
     3721 cgaacgctcc cggtgattgg ttgattctgc ctgtctacta tgatgagaat ttcaatttgg
     3781 ggagaatctt ccgcaaggaa acgaagtttg ataaatcatt agaaacaaca ttagaaataa
     3841 taagcgaaat cactcggcag ctttctacga tagcgtcatc atattcatct caaacactcg
     3901 caacaagcca agatgaatct tcaatgaata acgcaaatga cgttgaaaat agtacttcca
     3961 ttctcgagct accctatatc atgaataagg acaagattaa tggattatcc gtcaaattgc
     4021 atggtagcag tcccaacctt gaatggacaa cggctatgaa atttttatta accaatgtaa
     4081 agtggttgtt agcgttttct tccaatttac tatccaagtc aattacctta agccctacag
     4141 tgaatcacaa tgataagacc atcagcggaa acggatccta acccaagctt aagtcgactt
     4201 tgttcccact gtacttttag ctcgtacaaa atacaatata cttttcattt ctccgtaaac
     4261 aacatgtttt cccatgtaat atccttttct atttttcgtt ccgttaccaa ctttacacat
     4321 actttatata gctattcact tctatacact aaaaaactaa gacaatttta attttgctgc
     4381 ctgccatatt tcaatttgtt ataaattcct ataatttatc ctattagtag ctaaaaaaag
     4441 atgaatgtga atcgaatcct aagagaattc ccctcgaaga tcgatctggc ggccgcggat
     4501 ctttgcaaag atccaaaaaa agcatgcgta cggattagaa gccgccgagc gggtgacagc
     4561 cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg
     4621 cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat
     4681 ggttatgaag aggaaaattg gcagtaacct ggccccacaa acctcaaatg aacgaatcaa
     4741 attaacaacc agatatctcg actgaaaaaa aaggtttaaa ccagttccct gaaattattc
     4801 ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc tgtaaatcta
     4861 tttcttaaac ttcttgaatt ctacttttat agttagtctt ttttttagtt ttaaaacacc
     4921 aagaacttag tttcgaataa acacacataa ataaaccatg atgggagcag gggcgggtgc
     4981 tagcgagaac ctgtacttcc agggcgccca cgatgaagcc gtagacaaca aattcaacaa
     5041 agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg
     5101 aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga
     5161 agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac aacaaattca acaaagaaca
     5221 acaaaacgcg ttctatgaga tcttacattt acctaactta aacgaagaac aacgaaacgc
     5281 cttcatccaa agtttaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa
     5341 aaagctaaat gatgctcagg cgccgaaagt agacgcggaa aacctgtatt tccagggagg
     5401 cggatcttct agaccaacta ctgcctctga aaatttgtac tttcaaggcg agttgaagac
     5461 cactgcttct gaaaacttgt acttccaagg tgagttactc gagggtgaat tgaagactgc
     5521 tgctttggct caacacgacg aagccatggg catgaaacgg ttcttactca gtcgaagaca
     5581 gagacacctt cgtatgattt gctttcacaa tataagtctc tttagagcta atggtgactc
     5641 caaattgatc aaggaatatg gtgatggatt tataccatgt ttctttatct tggaatccat
     5701 aagaggggag ctgctttatg tgagcgaggt acaatcaggt tctttgcgta aactctcttt
     5761 tcaggaatta ccaaaactaa ctggtgcatc tactatgatt gtcctaaaat tggtaggact
     5821 agttccgagt gatattttgt gtaccataag ttcagataaa aatggtatca ttgacgataa
     5881 atggtgcgtt ttatgtacct acacgataga tcttaacaag ttacaaccca taaatgaaga
     5941 cacagttcta attacaggca ccaacgcacc agtacttgat ttaatagatg gatcctacac
     6001 attggctgca gaaaaaatta agccactaaa gggccttgtt agttctcaca aaaggaatat
     6061 aagtcaagtc aagatcaaat tttcattggc atatagctct ttactaaaat taaacaaact
     6121 attggagtat tcttcccaag tccatgagga aatcaatgaa atttccagca aaatagaaga
     6181 tgactttttg tcactcaaga atcaaaatca ttggtatatg agaacagtac agaagtccat
     6241 agaaacgcta gaaaaggaag tcctacaaag gaaaaaaagc aagaaaaata tagaaatggc
     6301 gcaactcgag agtaatgata ctatcaacca ttctaaaaca gaactgtctc tgatgtcgca
     6361 ggacgagtct ataaatgatg actatggaag catatattct agatttgtgc aaataaaaga
     6421 taggttggac caactgaggt ttaagaaact gtatcagctg atagggatct tccattcaac
     6481 ggatttattt aattcagata gaggctatat ttactttgaa aaaccttcta gcgtaaatga
     6541 tgttattaat aggctcaagc taaaaccttt gaatatagag atattactta gacaagccgg
     6601 cgaatccaca aagcacagag aatatgtaaa cagccagttg ggttattacc ttttatttct
     6661 ccatttgaca gccatccaaa ttttcaaggc tcctttaccc tatagactaa tgtattatgg
     6721 tagtacatca gtgatcgaca gtcaatatcc gttgtatttc actgatcaaa tgatttctaa
     6781 gcatcaagca aagctgatta aagccataca ttattttaat gctgatatat tgcaatttaa
     6841 acagattctg gagaattacc gtccaacagg atcctaaccc aagcttaagt cgactttgtt
     6901 cccactgtac ttttagctcg tacaaaatac aatatacttt tcatttctcc gtaaacaaca
     6961 tgttttccca tgtaatatcc ttttctattt ttcgttccgt taccaacttt acacatactt
     7021 tatatagcta ttcacttcta tacactaaaa aactaagaca attttaattt tgctgcctgc
     7081 catatttcaa tttgttataa attcctataa tttatcctat tagtagctaa aaaaagatga
     7141 atgtgaatcg aatcctaaga gaattcccct cgaagatcga tctggcgagc tccagctttt
     7201 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     7261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7321 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7381 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7441 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8281 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8341 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9361 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
     9421 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     9481 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     9541 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     9601 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     9661 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     9721 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     9781 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     9841 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     9901 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     9961 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    10021 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    10081 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    10141 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    10201 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    10261 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    10321 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
    10381 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    10441 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    10501 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    10561 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    10621 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    10681 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    10741 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10801 tttcgtc
//
