LOCUS       sequence-472832-        7261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector for Cre dependent expression of ChR2(CRC)-EYFP; CRC variant
            contains mutations L132C, H134R, and T159C in ChR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP11763: pAAV-hEF1a-DIO-ChR2(CRC)-EYFP-WPRE-H...
FEATURES             Location/Qualifiers
     source          1..7261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    150..183
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(214..1392)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          223..1161
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(246..266)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     repeat_region   1493..1622
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(1684..2272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1764..1783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2443..3234,3235..3303))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3066..3085
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3304..3408)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3476..3494
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3532..3554)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3654..3673
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3690..4145)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3827..3848)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4039..4058
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   4220..4360
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4231..4360
                     /label=AAV2 ITR
     polyA_signal    4400..4876
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     4658..4677
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    4908..5496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5014..5025
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5423..5443
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(5521..5554)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(5605..5638)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      5651..5660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5657..6583
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIRLSNLTGLSNDYSRRTMGLLVSDIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      6593..6602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6599..6601,6602..6604,6605..7261,1..57)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6644..6665)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6905..6924)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     join(7252..7261,1..12)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaggc
       61 gcgccataac ttcgtataat gtatgctata cgaagttatt aagaggtttc atattgctaa
      121 tagcagctac aatccagcta ccattctgca taacttcgta taaagtatcc tatacgaagt
      181 tattccggag tcgactctag aggatccggt acctcacgac acctgaaatg gaagaaaaaa
      241 actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa
      301 ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt
      361 gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa
      421 gctcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc
      481 gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc
      541 cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc
      601 cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg
      661 cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct
      721 cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc
      781 gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac
      841 aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag
      901 agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc
      961 ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg
     1021 cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag
     1081 ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg
     1141 ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt
     1201 cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca
     1261 gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt
     1321 gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg
     1381 ggcaccggag ccaattccca ctcctttcaa gacctagaag gtccattagc tgcaaagatt
     1441 cctctctgtt taaaacttta tccatctttg caaagcttac gcgtgcggcc gcaggaaccc
     1501 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     1561 ccaaaggtcg cccgacgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     1621 ggacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1681 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1801 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1861 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1921 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1981 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2041 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2101 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2161 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2221 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2281 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2341 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2401 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2461 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2521 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2581 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2641 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2701 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2761 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2821 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2881 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2941 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3001 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3061 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3121 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3181 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3241 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3301 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3361 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3421 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3481 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     3541 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     3601 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     3661 agagcagatt gtactgagag tgcaccataa aattgtaaac gttaatattt tgttaaaatt
     3721 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa tagaccgaaa tcggcaaaat
     3781 cccttataaa tcaaaagaat agcccgagat agagttgagt gttgttccag tttggaacaa
     3841 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3901 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
     3961 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4021 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaagg cgctggcaag
     4081 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4141 cgcgtactat ggttgctttg acgtatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     4201 aaataccgca tcaggcgccc ctgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc
     4261 cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg
     4321 cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgctc ggtccgcacg
     4381 tggttaccta caaaatcaga aggacaggga agggagcagt ggttcacgcc tgtaatccca
     4441 gcaatttggg aggccaaggt gggtagatca cctgagatta ggagttggag accagcctgg
     4501 ccaatatggt gaaaccccgt ctctaccaaa aaaacaaaaa ttagctgagc ctggtcatgc
     4561 atgcctggaa tcccaacaac tcgggaggct gaggcaggag aatcgcttga acccaggagg
     4621 cggagattgc agtgagccaa gattgtgcca ctgcactcca gcttggttcc caatagaccc
     4681 cgcaggccct acaggttgtc ttcccaactt gccccttgct ccataccacc cccctccacc
     4741 ccataatatt atagaaggac acctagtcag acaaaatgat gcaacttaat tttattagga
     4801 caaggctggt gggcactgga gtggcaactt ccagggccag gagaggcact ggggaggggt
     4861 cacagggatg ccacccgtag atctctcgag cagcgctcgg tatcgatgcg gggaggcggc
     4921 ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc
     4981 cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa
     5041 ggacgtcccg cgcagaatcc aggtggcaac ataggcgagc agccaaggaa aggacgatga
     5101 tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg
     5161 ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc
     5221 ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac
     5281 agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa
     5341 ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa
     5401 aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt
     5461 caatctttca caaattttgt aatccagagg ttgattatcg ataagcttga tatcgaattc
     5521 ataacttcgt atagcataca ttatacgaag ttattctttg caccattcta aagaataaca
     5581 gtgataattt ctgggttaag gcaaataact tcgtatagga tactttatac gaagttatgc
     5641 tagcgcggcc gccaccatgg actatggcgg cgctttgtct gccgtcggac gcgaactttt
     5701 gttcgttact aatcctgtgg tggtgaacgg gtccgtcctg gtccctgagg atcaatgtta
     5761 ctgtgccgga tggattgaat ctcgcggcac gaacggcgct cagaccgcgt caaatgtcct
     5821 gcagtggctt gcagcaggat tcagcatttt gctgctgatg ttctatgcct accaaacctg
     5881 gaaatctaca tgcggctggg aggagatcta tgtgtgcgcc attgaaatgg ttaaggtgat
     5941 tctcgagttc ttttttgagt ttaagaatcc ctctatgctc taccttgcca caggacaccg
     6001 ggtgcagtgg ctgcgctatg cagagtggct gctcacttgt cctgtcatct gcatccgcct
     6061 gagcaacctc accggcctga gcaacgacta cagcaggaga accatgggac tccttgtctc
     6121 agacatcggg tgtatcgtgt ggggggctac cagcgccatg gcaaccggct atgttaaagt
     6181 catcttcttt tgtcttggat tgtgctatgg cgcgaacaca ttttttcacg ccgccaaagc
     6241 atatatcgag ggttatcata ctgtgccaaa gggtcggtgc cgccaggtcg tgaccggcat
     6301 ggcatggctg tttttcgtga gctggggtat gttcccaatt ctcttcattt tggggcccga
     6361 aggttttggc gtcctgagcg tctatggctc caccgtaggt cacacgatta ttgatctgat
     6421 gagtaaaaat tgttgggggt tgttgggaca ctacctgcgc gtcctgatcc acgagcacat
     6481 attgattcac ggagatatcc gcaaaaccac caaactgaac atcggcggaa cggagatcga
     6541 ggtcgagact ctcgtcgaag acgaagccga ggccggagcc gtgccagcgg ccgccaccat
     6601 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     6661 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     6721 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     6781 cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
     6841 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     6901 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6961 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     7021 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     7081 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     7141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     7201 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     7261 g
//
