LOCUS       sequence-47284-e       15961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Voronina E, Seydoux G
  TITLE     The C. elegans homolog of nucleoporin Nup98 is required for the 
            integrity and function of germline P granules.
  JOURNAL   Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24.
  PUBMED    20335358
REFERENCE   2  (bases 1 to 15961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2010-05"; volume: "137(9)"; pages: "1441-50. 
            Epub 2010 Mar 24"
COMMENT     Sequence Label: pEV1.05
FEATURES             Location/Qualifiers
     source          1..15961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     624..643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        624..642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     668..684
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    9927..9951
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9984..10012)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10760..10780
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             10866..10886
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10899..10943
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    complement(14123..14147)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      complement(14211..14799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(14970..15761,15762..15830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15593..15612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15831..15935)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
      661 cccccctcga ggtcgacggt atcgataagc ttcagtaaaa gaagtagaat tttatagttt
      721 tttttctgtt tgaaaaattc tccccatcaa tgttctttca aataaataca tcactaatgc
      781 aaagtattct ataacctcat atctaaattc ttcaaaatct taacatatct tatcattgct
      841 ttaagtcaaa cgtaacatta aaaaaaatgt tttggaaaat gtgtcaagtc tctcaaaatt
      901 cagtttttta aaccactcct atagtcctat agtcctatag ttacccatga aatccttata
      961 tattactgta aaatgtttca aaaaccattg gcaaattgcc agaactgaaa atttccggca
     1021 aattggggaa ccggcaaatt gccaatttgc tgaatttgcc ggaaaaacgg taattgccga
     1081 aagtttttga cacgaaaatg gcaaattgtg gttttaaaat tttttttttt ggaaatttca
     1141 gaatttcaat tttaatcggc aaaactgtag gcatcctaag aatgttccta catctatttt
     1201 gaaaagtaag cgaattctat gaaaatgtct aaagaaaatg gggaaacaat ttcaaaaagg
     1261 cacagtttca agtgtttccg aattatacta aatccctcta aaaacttccg gcaaattgat
     1321 atccgtaaaa gagcaaatcc gcatttttgc cgaaaattaa aatttccgac aaatcggcaa
     1381 accggcaatt tggcgaaatt tgccggaagg caattgccgc ccacccctgt tccagaggtt
     1441 caaactggta gcaaagctca aaatttctca aattctccaa tttttttttg aattttggca
     1501 gtgtaccaaa atgacattca gtcatattgg tttattatag atttatttag ataaaatcct
     1561 aaatgattct acctttaaag atgcccactt taaaagtaat gactcaaact tcaaattgct
     1621 ctaagattct attgaattac catcttttcc tctcattttc tctcactgtc tatttcatca
     1681 caaattcatc cctctctccc tctctttctc tctccctctc tctctttctc tttgctcatc
     1741 atctgtcatt ttgtccgttc ctctctctgc gccctcagcg ttccccacac tctctcgctt
     1801 ctcttttcct agacgtcttc ttttttcatc ttcttcagcc ctttttcgcc attttccatc
     1861 tctgtcaatc attacggacg acccccatta atttttggat gtgctctgcg agcaagagca
     1921 cacggaactc atttattttt tgttggaatt tctttttcat tttagttagt taaaaggtta
     1981 ttcagtgcaa aaattgattt tttgctcttt tggtacatta gaggggtttg aaagttttcc
     2041 ataaaaaggg tttccggagt aaaaaatcta aaaattttta caaacatttc caaaaatttt
     2101 gggaacatat cttttcaaaa actcaaaaaa aaacgcaaac atcataaatt taatcaatta
     2161 attggatgcg ttcaaatatt ttaaaaatat ttttgaattg catatttctt tactcataaa
     2221 aaaattatta atgtttaagt aaaaaaaata tttcaggatt ttaaacaaaa actgtaaatc
     2281 ccataaaaca gtgttatttt ttagttattt ttagtaatta tttgtgaatt aaaaacacat
     2341 tttttttcaa aatattcctt gaataaataa aattgaaagt gaattcaaaa tatttcacca
     2401 attttacaaa atttgaaaat ttgaaaaaat attttctacc aaattgtgct caaaaaatat
     2461 tattaagttc ccaggaaatt tatgatgaaa acttggaata tttttggtaa aattttctca
     2521 aaaaattgaa agttgtcaaa aacacactaa cactatacct cgcggcatag aaaaaactgg
     2581 tggccgaatt ttttaaacca atttaaatta aatatatttt tgcactttgt acaaaaaaat
     2641 ttgaagttcc caggaaattc atacggaaat attttccaga acttttataa tttttatttt
     2701 aattctcaaa atttacaata aaaacttgga aacctgaaat ttgcttaaag aataacccta
     2761 tcccccacgg cctagaaaat actggtggcc gaattttttc cgcagccaca ccacctctaa
     2821 tctccagagc actctccaaa attcccataa tccccaaaat tttcagctac aacagcatat
     2881 atgaaggcag agcaacaaca acaatcgatc gcacccggct cggcaacctt cccgtctcag
     2941 gtgaggaaga cgcagagaaa tgagagagag agcgtgttta tgcacgcagt atactaactc
     3001 ctgatatcca atttcatata tcgatatgct tgctttcttt tcttctacgt tgaaattgtt
     3061 tgaacttttc tcaaaaaacg cggattttag agagacttag agttgctcca aataaattta
     3121 atttgcaatt ttgcacttac tattagaatc tacttttata gcgctaaaat gaccgaataa
     3181 cacagtagat tttaaaagaa aattttaaaa attatttttt tgtgcagtca aatttcgcta
     3241 gtttttgcca ctcatttttg aacatttgta tatgtgcctt tcggtggttc aatcaggggt
     3301 ggcacgtggt gtcagagtgt tccatttcgg tttgatctac gttgatctac aaaaaatgcg
     3361 ggagaagaga cgcagagttc tcaactgatt tcacatggtt atgaaccagg ggtgttcggc
     3421 aaatttgccg tttgccgagc tgggcaaatt ttgagatttg ccgcacaccc cttttatgaa
     3481 cgtgctgacg tcacatcttt ttggacaaag aattcccgca ttttttgtat atcaaaccgt
     3541 gatgggacag cctggcacca cgtggggttg gcggcaaatg attttttccg acaaatcggc
     3601 aaattggcaa attgccggaa tttaaagttt ctggcaaatt gttgatttgc cgaaaaaaaa
     3661 tttcaaaaag gcacagtttt aagtgtttcc gtcttataaa atatttctct aaaaatttcc
     3721 ggaaaacgga aaattgccgg aaatacaaag tttcgtcaaa tcggcaaacc tgcaatttgc
     3781 cgaacggcaa ctgccgccaa cacctggttc ccaaaaaagc gtgcactgtt tgaatattaa
     3841 aaaaaaaatt cacacaaaaa taataaattg aaataggcta aattcacatc tatgtacaaa
     3901 aattgcatgc cagcaccggt caattttctc aattactttt tgtctatttt tgccgaacat
     3961 aatttgctgt ttccttggca gaatacagaa aactcttttt tttttcagat gccccgaccc
     4021 ccaccggtaa ccgaacaggc tataaccacc gaggcggagc ttctcgcgaa aaatcaaatt
     4081 acaccaaatg atgtgctagc tcttccagga atcactcaag gtaagtgaat atttgctcta
     4141 ttgataaacg cgccgtgtac tccacgtgga caaacatatc aaattttcag gattcctatg
     4201 ctccccatcg gcaaacgtct ataacattga gttcaccaaa ttccaaatcc gtgacctcga
     4261 cacggaacac gtgctcttcg aaattgcaaa accggaaaat gagacggaag agaatctgca
     4321 ggcgcaagcc gaatcggcaa gatatgtccg atatcgattt gcgccgaatt ttctgaaatt
     4381 aaagacggtc ggcgcgacgg tcgagttcaa ggtcggcgat gtgccgatca cacattttcg
     4441 aatgattgaa cggcactttt tcaaggatcg tttattgaaa tgctttgatt ttgaatttgg
     4501 attctgtatg ccaaattcga gaaacaactg tgaacatatc tatgaatttc cacaactttc
     4561 acaacaactc agtgagtttg aagcgggaaa aattatttga gctcattttg tcaatagagc
     4621 gaattttcta ggtttttaaa aataaaataa atattgacaa aaagctgccc tacagtagtt
     4681 caaaatgtga aaacttggag gaaattcgag ttttctctga gaagtttttg gcgggtaatt
     4741 caaattttct gagaaaaaaa ttagccgcga gaattcaaat tttctgagaa aattatttga
     4801 cgggaaattc aaaatttctg gaaatgtatt gaacatttga gaaattttgt taaaaactac
     4861 attaaaccga taaaaaagtt cgcaaatttt ttttaagtgc tcatggtgca tcgaagagaa
     4921 gaattcttgc caaaactagc ccatgaaatt aaaaattatc gcggaaaaaa actgactaat
     4981 tggaaagtaa aatgaaattt gcaaaaaaaa aatatgtttt tttgttgtaa aattaaaaaa
     5041 aaaactgtat attttcagtg gacgacatga tcaataatcc aaacgagacc cgctccgata
     5101 gcttctattt tgtggagaat aagctcgtaa tgcacaataa ggccgactac tcgtatgatg
     5161 cataatttcc cgccgatgga ataaaactaa taatttttct cgcaatttat agaaatcatg
     5221 tgattttcct accgctaaat ttaaaatttc ctaatttttc actatatttc tcacaaaatg
     5281 cattccgaaa actctcattt ttcagctttt caatacaaat cccaataaat gatttttcaa
     5341 atataatgtt ctcaattgat atgttttatt tgaatgttgg aaaaaaccat aaaattaaaa
     5401 aaaacttgaa ttattcaaaa ataattcggc ccttttacaa aaaattatca taaaaattaa
     5461 atttggaaaa tctgtgattt gtcgctttgc cggaagtttc caattccggc aactaaccga
     5521 tttgccgatt agctagaaat ttcaattccg acaatgtgcc gctttgccga tttgccggaa
     5581 atttccaatt ccggcaatta acggacttgc cgattagcta gaaatttaac tttagataaa
     5641 acttctcaac aaaatcaaat tggaaagtca gcgatttgcc gctttgccgg aagttttcag
     5701 ttccggcatc taaccgattt gccgatttgc caaaaatgtc aattccgaaa aactgccgat
     5761 ttgccgcttt gtcggaaatt tcaattccga caaattgccg atttgccgat ttgccgaaca
     5821 ttccaattcc gaccatttgt cgatttgccg gaagttacca attccggcaa ctaacggatt
     5881 tgccgatttg ccggaaaaaa attgtttgtc gccaaccctg atttaaatga ctaatcatgt
     5941 tgcaaaagat aactgcttgc atcaaaaatc cgatcattgg cattcccaac atcttcttca
     6001 tccaaaacac cacccttttc ttccactaat catgaattcc cgaccaattg ttatcagtag
     6061 gagaagcctc cttttatctc attatcagca attgccttct tcttcttctt ctttgaatac
     6121 catttgaatg gagatatcat ttacataaat ctgccgccga gattgtgaag caggaggaaa
     6181 agctgaagat actgatatta tatgtatatt tcattattct ttttccacaa aattttccga
     6241 catttaattc cacttcactt tcatcgtctc tttttccccc attgtcgccc ccactcattt
     6301 gtcttcttct tcaaatacgg gtcaatcact ttccgttttt tattcatttt ttgttatgtt
     6361 cggcttaaca aatttgaagt attgttaaaa ttctctagag cggccgccac cgcggtggcg
     6421 gccgcgcgga agatctggaa gtaaaatatt gtaatatttt ccctaaattt cacttaaaaa
     6481 taaggatttt tgcaacgtaa aacctcaaaa attcgctgaa attgacgatt tttgttcaaa
     6541 attttgaaaa acagggttaa ttttagctga aaatcttcaa ttttgccaga aattggcttt
     6601 ttttccgtcg ccacacggag aacttgccaa aaatgaagac ttttagccaa tttagccctg
     6661 aaaattcgag ttttgggcga tttttggtct gagaagccgt tttcgcagta tattatattt
     6721 tctcgttaaa tttcatttta aaataaacat tttgaacgta taaccccaaa aattcgctgc
     6781 gcaatggatg atttttgtcc aaagttttga taattttagc taaaaacctc gaattttggc
     6841 aatttttcgt gccgcatcgg ccgaaagttg actttttttg gaattatcat aatttaatgc
     6901 acattttggt agtttatctc acataatctg tctgcaggcg ggaaaaattt gagataaact
     6961 actaaaagat gcaaaacaag gatgataatt ccagaaaatc aacttcggac cgtctcgaca
     7021 cggaaaattg agcgaaattg gagttttaaa atgatttagc cctgaaaatt cgagaaattt
     7081 aggcggtttt gggtctgaaa aggcgttttc gcagtatttt ctcgctaaat ttcatttatt
     7141 atcacataat atcagtactt aggcgaatat cttgtagaaa tgttggtttt gttcgataat
     7201 tctctacttt tagagtcgat tcacgagaaa atgttgcacc atcttacttt tttcccttgt
     7261 aaaacataga ttcttctgta caatgaaaga gaacacaatt tcttcgtgaa gcaactcaaa
     7321 aagtagagaa aattcgaaca aaacccaaca tttctacaag atattcgtct aaatagtgat
     7381 ataatgtgtt aataagcata ttttaaatga aatttagcga aaaaactgcg aaaatttgtc
     7441 aaaaaagcga atttccatcc gatcagcaga tctctaaaag ttacataaaa ttgaaagttt
     7501 tgtggggaaa ttgttttaaa acacgcctat ggtaatacga gaacataaaa ttctgagaat
     7561 gcgtattaca caacatattc gacgcgcaaa atataactat atctcgtagc gaaaactaca
     7621 gtaatcctta aatgactact gtangcgatt gtgtcgattt acgggctcga ttctcggaaa
     7681 aaaaaatcat ttttcaaatt ttgacagcga tattcaattt ttcttcgttt tttcgtatnt
     7741 tctcagtcat ttttgtgctt atttttaata ttttattcat taataaattn aatttaattg
     7801 aaaaacgtcc agacgtattt ttacatttcc cgtctatttc cgtaataggc ggttaataac
     7861 gtagaaatac gtctgtatga gtttactcat caaaatgaca cgaatattgg acgtttttaa
     7921 atcaaattaa tttattaata ggtaaaatat taaaaaataa acacaaaaat gacaaaggaa
     7981 taggaaaaca cgaagagaaa ttgagtatcg ctgtncaaaa ttcgaaaaac gtggattttt
     8041 ccaagaatcg agcccgtaaa tcgacacatt cgctacagta gtcatttaag gattattgta
     8101 gttttcgcta cgagatactt tgcgcgccaa aaacgtggtg aaatacgcat tctcagaatt
     8161 ttatgttccc gtaataccac aatgaccgaa tatcataata aaaaaattca aaaacaattt
     8221 ctagatttta tatgattttc tgaaaattga aaaaatctca gttttcacct aattatattt
     8281 gaactacagc caattgaact cgttcgttgg agcgcgcttg cattattttc attaattaat
     8341 tttattaatc ctcattattt cactgatttt cttcattttt gaggtttttt ttatcgggaa
     8401 atgaaagaaa tatacaagaa aaatgcaaaa tgtttattaa aaactgaaat tagtatttct
     8461 cggagtttta ggcattttca gttacttttt aataaagatt ttgcattttt cttgtatatt
     8521 tctttcattt tccgattaaa aaaccccaaa aatgaagaaa atcagtgaaa taatgaaaat
     8581 taattcaaat aactaatgaa aataatgcaa gcgcgctcca accaacgagt tcaattggcg
     8641 gtaattcaaa tataattagg tgaaaactga gattttttca attttcaaaa aatcatataa
     8701 catctagaca atttttttga atttttttat catgatattc ggtcattgtg tccccatagg
     8761 catgttttaa agcaatttcc acctttaaat aaaatcgaga aaaaatggcg tcaaaagaca
     8821 tatgtaaaag ctgtttctct ttgtatattc acacagaata tagcccgatt ttggaggtga
     8881 aaaaccggtt tttttgtatt ctttacgcca cataaagtga tatggaagag aaaatcgaca
     8941 tatttatgta cttggagtac gagtacattg gagaaaaagg gtgaactact ggaaaatctg
     9001 ggaaatttca gaaaaaaatt ccgaaaaatc ttttttgctg aaaaaaaaat ttgttcgaaa
     9061 tttgggaaat actggaaaat tttcatcaaa attaaagcga tttttaacgt ttttgcttgg
     9121 gagatactat ttgtaatttt aatgtttggt gaggacaaca gaaatgacct tcagcgtacg
     9181 gaaaacgatt aaaagacaca tttgaatcga aaatagcgtg aaaaacatga aaatatcgga
     9241 aaaatagctt taaaattgga tttgaaagca aaaaatgtat caaaaatcga gaaaaatgct
     9301 tgaaaaagtt gaaaaactca aacaaaaaat caaaaaaggt ttttttaaaa atctgtgagt
     9361 atcgcaacga aagcacaact tgaggcatgc gcctttaaac caaccgtaac caaattcgga
     9421 ggcaaaaaaa gatttctcgc tgttttttca gttttaaaat cacttttcac gtttcttttc
     9481 gcagtaattc gtttgcaaaa catctgaaaa aattttattt tataagaaat tatataaaaa
     9541 tcgctataaa ttgagttttt gcccccaaat tcagcacgga gcacttctca attaaatata
     9601 attttatttt ttaacattga aagctttcat aaaattgagc tttgtttgta aataatcagt
     9661 gaaaaaacac agaaatcttc aaaaaataaa gaaaaagttt ttaaaaatct gtgagtcccg
     9721 caacgaaaat aaaactttag gcatgcgcct ttaaaccaac cgtaaccaaa ttcggcggca
     9781 aaaatggatt tctcgccgtt ttttcagttt tacaatcact ttttaggttt ctttttgcag
     9841 tatttcgttt cccaaacaat taaaaatcaa attttctttt ccagatggct caaacaaagc
     9901 cgattgccga gcaaatggga tcatcaacaa gtttgtacaa aaaagcaggc tcaatgagta
     9961 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
    10021 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
    10081 cccttaaatt tatttgcact actggaaaac tacctgttcc atgggtaagt ttaaacatat
    10141 atatactaac taaccctgat tatttaaatt ttcagccaac acttgtcact actttctgtt
    10201 atggtgttca atgcttctcg agatacccag atcatatgaa acggcatgac tttttcaaga
    10261 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
    10321 acaagacacg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
    10381 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
    10441 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
    10501 atgtatacat catggcagac aaacaaaaga atggaatcaa agttgtaagt ttaaacatga
    10561 ttttactaac taactaatct gatttaaatt ttcagaactt caaaattaga cacaacattg
    10621 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
    10681 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
    10741 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
    10801 gcatggatga actatacaaa tgcccggggg atcggtggag ctccaccggt ggcggccgct
    10861 ctagagagaa tctttatttt cagggcgccg ccaaattcaa agaaaccgct gctgctaaat
    10921 tcgaacgcca gcacatggac agcggaggtg gaggtatgga ccaatcaaag atgcgcagaa
    10981 cgaatcagtt cagaaaagta agttttcagt aacttctaaa gaaacaaatg gcctaagttt
    11041 tacagacttc ccagaagcct ccttctacag ggattgacag ttatccgact cccgcacaat
    11101 caccaatggc gcagcatgag acacctatgt gggacttcaa ctctctcaac ccgtatttct
    11161 caatgctcaa tatgaacgat gggattaatt atgctcgcca tcaacagaat cacattgtta
    11221 catcgcggcc accaaccccg ctaactgatt tgatgagcct tcggagtttt cagagctttg
    11281 taagttagag taggcaattt ttggttgttt tcattactga tttgcagccc aatgtgttca
    11341 tgccggtctc gcgtagccga acttcatcat tcattcagtc tgatacggat tcctcgcgtc
    11401 tcgagtcgga tgatttttca caaaatgtcc gatgtttctc tgcggatatt gatcgtagca
    11461 agagctatgg ttcttcaaaa cactaccatt tgaaatattc gaggttcgaa ttaatttatc
    11521 actatcctct agtatttatt cttttttatt tatagacccg ctctgtcgag gaactctaga
    11581 tcattcacaa gatcaaacaa tgtgctgcca acctggtcct tggattccaa tggtgaaatg
    11641 cgttcaagat tgtcgctctc tgaagttttg gatagtggcg atttaatgaa gtttgccgtt
    11701 gacaaaactg ggtgccagtt cctagaaaag gcagtgaagg gcagcctgac atcttatcag
    11761 aagttccaat tgtttgaaca agttattggg agaaaagatg atttccttgt aagttttagt
    11821 ttctgcgagc acacatgact tttcttttca gaaattgagc acgaacatct ttggaaatta
    11881 tttggtacaa agcgttattg gaatttcatt ggcaactaat gatgacggat acacaaagcg
    11941 acaagaaaag ttgaagaact tcatcagttc ccaaatgaca gatatgtgct tggacaaatt
    12001 tgcgtgtcgt gttatccaat ccagtctcca gaacatggat ttatcccttg cgtgtaaact
    12061 tgttcaagct ctacctcgtg atgctcgtct tatcgcaatc tgtgttgatc aaaatgccaa
    12121 tcacgtcatt cagaaagtcg tagcagtcat cccactgaag aattgggagt ttattgtgga
    12181 tttcgttgcc accccagagc acctccgtca gatttgttct gataagtatg ggtgccgtgt
    12241 tgtgcaaact attatcgaaa agctcactgc tgattcaatg aacgttgatt taacttccgc
    12301 tgctcaaaat cttcgtgaac gagcgctcca acgactcatg acttctgtga ctaatcggtg
    12361 ccaagaatta gctacgaacg agtatgccaa ttacataata cagcatattg tatccaatga
    12421 tgatctggcc gtttatcggg aatgtatcat agagaaatgc ctcatgtgag tgttattgtt
    12481 taatatctac ttgattttta atcatcaata tttcaggcgc aaccttcttt cgttgtccca
    12541 ggaaaagttt gcttctcatg ttgttgagaa agcattccta catgcaccat tggaacttct
    12601 tgccgaaatg atggacgaga ttttcgacgg atacattcca catccgtaaa aacattagtt
    12661 tcagtcacat aaaaatgaaa tgttttttca gggacacggg aaaagatgcc ctcgatatca
    12721 tgatgttcca tcaattcgga aactacgtcg ttcaatgcat gctgactatt tgctgtgatg
    12781 ctgtgtctgg aagacggcaa acgaaagaag gaggctatga ccatgcaatc tcattccagg
    12841 attggctgaa aaagttgcac tcgagagtta cgaaagaaag gcatcgcttg tcaaggttat
    12901 cactattgtc acaataatat tttgaaattc gaaattcagg ttctcatccg gcaaaaagat
    12961 gatcgagacg cttgccaatc tccgttcaac tcatccgatt tatgaactcc aatcttcggg
    13021 acacgattca ttcaagacgg attacttcag tactgcttcc gagcacgatg gtccggagtt
    13081 ggagaagaat gggatcgagg aaggaagcct gatgctagag ccacggagca ataagtcttc
    13141 agtctctgtc aaattttctt cttccggtag tcacggcgat gattaaggtg gaactttctc
    13201 accataaatc tcatcccacc cacttatgtt ctgttgtttt gtaatatttt aaactggtgc
    13261 aatgatttcg tttcgatttt aagatcgatg ttgtgccttc cccactctta tcattgccca
    13321 gttttgtttt ttcttttctt ttttgttatt ttattgcttt ttatgcagtt gtgttattgt
    13381 ttctttctgt ttactgttca ttgtttttgt taatttttta ttttcgattt taatgaatcc
    13441 agataaaatt agacaacttg gcaaaatatc gttcgagtct ttgtcatgct tccaccgaga
    13501 gcttcctgct tccatacaat gcctgctcag aaaacaattt tgaaagcgaa gagaaaagtt
    13561 gttctttgct cggagaacaa aaaactgata accgacaaat gttgactttg taaatttaat
    13621 caaattagaa accatgaaga ataaatatag ttcaattatc atattgaaat ttatcttaca
    13681 attgcatcga tttcaaaata atgggactct cagaaaatag aaaaacaaaa attggatact
    13741 ggggatgagg aagaactccg cgaacattgg cggtccttct ctcatgtggt cctcgtatag
    13801 gtggtggcaa tcctctcatt gttatccaac atcgagaaaa tgttcttcgg ctagaactct
    13861 gaaacaaaaa tactttcaaa ttaaaattgt caagataaat tcgtacttcc tgacacaatt
    13921 tgtgttgttg cagcgttgaa ttcgattcca aaccaagtgg caagaccaag acccagcaat
    13981 gatgatggaa tagttgaatt gacagaattc ctcgagcata tcagcaatgg atgttgatgc
    14041 aagtggatgc aatgttcttc ttttgtttct catcaacgtt tgccttatga ttgtgaagtg
    14101 acttgatgaa ctctctctgc catgccagct ttcttgtaca aagtggtagt catgtgagca
    14161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    14221 ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    14281 acaggactat aaagatacca ggcgttcccc cctggaagct ccctcgtgcg ctctcctgtt
    14341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    14401 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    14461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    14521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    14581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    14641 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    14701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    14761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    14821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    14881 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    14941 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    15001 tcagcgatct gtctatttcg ttcatccata gttgcctgac tgcccgtcgt gtagataact
    15061 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    15121 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    15181 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    15241 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    15301 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    15361 acatgatccc ccatgttgtg aaaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    15421 agaagtaagt tggccgcagt gttatcactc atgcttatgg cagcactgca taattctctt
    15481 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    15541 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    15601 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    15661 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    15721 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    15781 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    15841 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    15901 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    15961 g
//
