LOCUS       sequence-472847-       17875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17875)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 17875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO379
FEATURES             Location/Qualifiers
     source          1..17875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2033..2057
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(2083..2270)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     2191..2215
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(2293..2466)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(2467..2640)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(2656..2676)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(2758..2778)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(2800..2820)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    7524..7641
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    10918..11035
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             complement(13138..13158)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(13180..13200)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(13231..13251)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(13261..13434)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(13435..13608)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(13624..13644)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    13981..14098
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    complement(14186..14210)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(14273..14293)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14273..14291)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14312..14328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14312..14328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14325..14347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14336..14352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14360..14366,14367..14384,14385..14390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14405..14426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14543..14560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14714..15302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14794..14813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15473..16264,16265..16333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16096..16115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16334..16438)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16465..17807
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(17428..17475)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17848..17866
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgg gccccccctc gagtacttct cgacaagcta tcaccacttt
     2041 gtacaagaaa gctgggtcta gatatctcga gtgcggccgc tgccggtaga ggtgtggtca
     2101 ataagagcga cctcatgcta tacctgagaa agcaacctga cctacaggaa agagttactc
     2161 aagaataaga attttcgttt taaaacctaa gagtcacttt aaaatttgta tacacttatt
     2221 ttttttataa cttatttaat aataaaaatc ataaatcata agaaattcgc gagctcacga
     2281 attcgcgtct actttcggcg cctgagcatc atttagcttt ttagcttctg ctaaaaggtt
     2341 agcgctttgg cttgggtcat cttttaaact ttggatgaag gcgtttcgtt gttcttcgtt
     2401 taagttaggt aaatgtaaga tctcatagaa cgcgttttgt tgttctttgt tgaatttgtt
     2461 gtctactttc ggcgcctgag catcatttag ctttttagct tctgctaaaa ggttagcgct
     2521 ttggcttggg tcatctttta aactttggat gaaggcgttt cgttgttctt cgtttaagtt
     2581 aggtaaatgt aagatctcat agaacgcgtt ttgttgttct ttgttgaatt tgttgtctac
     2641 ggcttcatcg tgggcgccct ggaagtacag gttctcgcta gcacccgccc ctgctccgga
     2701 tctagcttcg tcgtgttgag ccaaagcagc agtcttcaat tcaccctcga gtaactcacc
     2761 ttggaagtac aagttttcag aagcagtggt cttcaactcg ccttgaaagt acaaattttc
     2821 agaggcagta gttggtctag aggatccttg gaagattcca ataagccctg agttatcaca
     2881 agcaaccagc aaaggtgtct catcaacttc acatgtagat atagaattta taatatcatg
     2941 atgatataga gaatcatcta ctgaatgagt gctagtacgt ttcatttttc tcaataacat
     3001 tgtgagattt gccgtaactt gtgtaggaat aaaaacgtca ctgaatcttg aaggacttat
     3061 tactgcttta gaagaagaaa gctcatttag gctaaacata acaatggaac ttgttgcttc
     3121 atcggtaaga agaattttat cattagatac tgagatagtg cttagtgcgt ttaaagacct
     3181 gattccacaa aaatttaatt cttctaagcc tttctcaatt ggtaaaaagt gctccataga
     3241 tggctgttca tcagaattta tgaaagcata ctgacaatgc cccttaacaa aattccatat
     3301 tgttagaaat gtttttgaac tacctcctac aacaattaca gaattctttc cataaaactg
     3361 acaaacctcc acatgcgtga ttggtgcgtg gtccccaaag gaccaactcc ttatcagcac
     3421 gttgaaacgg atatcccata tatcaataat acctctagtc gtccccaaaa ttaggacaca
     3481 acactcttca tcgatacaga tgcttgaaac ggcaccatgc cttggagaat tctctataat
     3541 ttgtaacctc tccagggttc taatatcaaa tataataacc cttgacaaat tcgtcaatgc
     3601 tactagtaga gatttttcct cattcacaaa tgctctcatt ctcactgcgt attcattttt
     3661 accaaaattc ttcaagttaa ttttcctgat gcattcgcaa ttcaaaaatt tgacttcact
     3721 ttcttgttgg taatgattaa cctttaatac aattatttgt ccatctttac tggaaacggc
     3781 aaacgcgtca aagttaggaa tcatggttat ctgagttacg gtagaggagc agtcataagt
     3841 taaagaagaa gagtaaacct cgcccacgat aatctctttc aggttccaaa tcttgattac
     3901 accttgatct gaacctgtta ttaaataggg tgtttctcct ggagaaacag cagaagacgt
     3961 aatagaattg ggttcgtttt ccatcaaagt agctatcaac ttgcccctca aattacccat
     4021 gtttggactc cgtacaatct cttgaatagg cccaaactcc ttataatctc tcagaggagg
     4081 taaaattttg aaagtcgata ggaatttttc tatgctttca acgtcgcctt catagctgtt
     4141 gctgacgact accttccttt ctggtttaac atttgcatta tctttcaagc catgatttgg
     4201 agaatgcatg tgataggacg ttggttctaa ctgaacaaaa acgttcttca aattagaagt
     4261 agtagtggca atggacttat ttttgaatat tagtgatcca tttatgtcca tgacggtaga
     4321 tagctgtttg ctaccgttaa tatttagctt attgctatct ttctcacttt catcatattt
     4381 atatatctgt tggttctcta agttagaatc ttgtccctca cttgtcgact cttcagcaaa
     4441 ctctacatca aagaaaatgt ttcttggcat aatattcgta aggtttaggt taggtggtga
     4501 gttctgtact aatggacggt aattactatt attatatgga aaatcagggt tcgctgccat
     4561 aactctcgct gtccttatta cataacccct caaagccaca attttccaaa tatctttttc
     4621 tgttagccca ataatgtgga acttatcaat ccaatttctg tcttcagcag ttaagggaat
     4681 ttccttgttg tcatgtgaat agacggtgga tgatgtttta atacccttca gcgaggaact
     4741 tgatttcgta tctcttttat taaatccata atttttactt gagtaatttt ttgttataaa
     4801 ttcgattcta ttatttccga atgaatctac atgatttgtg atgatttttt tccaaaataa
     4861 agattttgac gctctaacag accaactaca caatagattg taaaccgatc ttgacactgg
     4921 ttgcttgcaa cagcttatca ttgatttgaa gttgaactca acgtcaaatt cgaagaaagg
     4981 ccttattatt ggatagagaa tgcagtacac ttcggctttt gataacttat taataatttc
     5041 tataattatc atcaaagtaa actgtcttat ccaattatta ggatgcaaca acaagggaga
     5101 tgttgttttt gatatatcaa tataatattt tttcctaatc aacccagtct tgaacaaaga
     5161 ttttaagctt tgtaaaacac tgatcactac taattcttcc gaatcagtga tggtctggat
     5221 taacaatggt aaaatatact gttctaatgt aacggtaccc aaaagaattg atattccgga
     5281 tattgtttga attaaggaaa ctcgcaatgc tgggtcctta tcattaaggt aggttattaa
     5341 atgactcagt ataatatcgt ttgttctttc cctaccaaaa aatttacaaa gtggaagaat
     5401 gttttgcaaa agtgccatct ttacataagt atcattatct gttaaaaaag agacagttaa
     5461 atcttcgaca ctttggatca attttgctga atacttggta ctgctttcca tgatttccgt
     5521 gttgttatcc attgagttat cattgcagtg ctgagcaaat gtaaattctt gaaatctatt
     5581 aatgataatg gccaagtcgc tcaaacaatt agcaaacaca atccttaaat aattggtatt
     5641 ctgcctattg gaaataagta atcttttcag tctcggaagt aagtagtcca caaatatatt
     5701 ctcgttcaat tgattcaatt ttcttacaga ggtgagtacc tggattaatg ttaacaaaga
     5761 tagggcctgg acgtcctggt cactatcttc aaaacaacat acgaaataag gtacgactcg
     5821 atcaatttta ttttcatcag aaacgaattg tgcaaatact gctaatagtt ctaaattttt
     5881 aagtttcgta gcagtcgaaa caatacttct tatactatga gacaaataag agataaagag
     5941 taaggcacat tcctctttaa ctgattttat cttttcggat actttggtaa actgctgtaa
     6001 gactaaatgg taattttggg ggaaatataa gtttgtgttt aaaaaccgac taatttctat
     6061 ctctattttg taagattcca aaattggggg gtctgaacca atctcacccc cgtcttttat
     6121 taaaggaaag tccaaacaat gacatatttg ggaaaaatct ctgtatattt tttccgtagt
     6181 ttcatctaaa agttttacat tgtcttccaa ggtactattg gtgcaagtgt tatctgatat
     6241 cggtgtgctt gttgtcatag taaccaaatt tctgaaataa tcataaatga aagtgtagaa
     6301 ataatcgggg aagaaaatgc cacgatattt attcagtagt tcatcacatg aaagtctttt
     6361 ggatggatct agttgaatca tgtctagaac caagttcctt aaatcggtag aattcatttc
     6421 ctccatgaga aattccctgt ttacgtcata tgaattactt ttatatttga atagctgtga
     6481 taagttgaag atgggtcttc cttcagcaaa tatttctgca ataacacatc caagactaaa
     6541 tatgtccatt tctttagtta gcctaccatt gttagatttt ccatcttggt aaagtttaga
     6601 gttaaacctc tccggggcta gataacaggt tcttctcttc gaggtgtcga agtagaataa
     6661 aaattcacct ggattatctt caggcaaata tacgggtttg ataaatgcag caaaatctgt
     6721 caatatacac caattccaac ttgttactag gatattttct gtctttatat caccatggac
     6781 aatattcaga ttatgaatgt cctttaatgc atttaacaac tggaaagcaa tgaatttcag
     6841 ttcaatgtct tgtaagtaag gtctcaaact caatctgtca tataaattat tttttaagtg
     6901 ctgccgtatc atgtagccgg ctctatttgt ctcgatcaat ttactgtagt ttaaaacgtg
     6961 cggtagttgt cccaacttaa acgattgagc ccttatacgt tggagaaaag gtcgtaagct
     7021 atattggtct tttggtttaa taaacacttt gataacaatt tcgccgttag ggtccagtgc
     7081 cttgcatgtt tttaagaatc ttgatgagtt taactgtgaa acgtagtgta cttcctctaa
     7141 gacatcgata tatgaaaaaa tggctatgga aggtgatgct tggaccacta gtgataattg
     7201 tgcccccatc tcgaggttta tttatgtgtg tttattcgaa actaagttct tggtgtttta
     7261 aaactaaaaa aaagactaac tataaaagta gaattcaaga agtttaagaa atagatttac
     7321 agaattacaa tcaataccta ccgtctttat atacttatta gtcaagtagg ggaataattt
     7381 cagggaactg gtttaaacct tttttttcag tcgagatatc tggttgttaa tttgattcgt
     7441 tcatttgagg tttgtggggc caggttactg ccaattttcc tcttcataac cataaaagct
     7501 agtattgtag aatctttatt gttcggagca gtgcggcgcg aggcacatct gcgtttcagg
     7561 aacgcgaccg gtgaagacga ggacgcacgg aggagagtct tccttcggag ggctgtcacc
     7621 cgctcggcgg cttctaatcc gtacgcatgc tttttttgga tctttgcaaa gatcccccgg
     7681 gcgccagatc gatcttcgag gggaattctc ttaggattcg attcacattc atcttttttt
     7741 agctactaat aggataaatt ataggaattt ataacaaatt gaaatatggc aggcagcaaa
     7801 attaaaattg tcttagtttt ttagtgtata gaagtgaata gctatataaa gtatgtgtaa
     7861 agttggtaac ggaacgaaaa atagaaaagg atattacatg ggaaaacatg ttgtttacgg
     7921 agaaatgaaa agtatattgt attttgtacg agctaaaagt acagtgggaa caaagtcgac
     7981 ttaggtccgc cagtattgtg ccagattatg taaatgatca atcacgatag gcagcaaagc
     8041 atttacacta tcattgatta gattctgaaa atgcactgtg gcatcttctt cggacatatt
     8101 caaattaaat ctctctctta cacgtaatat agcaccattg ggatctattc tgatatcagg
     8161 tatgttcgac gtcttcatta attcaaacag gtttaagatt aagcctgcat tccttcttaa
     8221 aatcgaatat gccacaaaac agtagctgcg aaatttatca taatttgatg attctgcacc
     8281 cccaaacgcc tctataattt gagggggcaa tttcattaat ggcggaaaag gtttggggtc
     8341 ctgacccaag atataaccaa agtctgcgtg aaaaaagtgc ccatctggtg tgactagtaa
     8401 gttgtctaag tgcctatcgc cgacacctaa gatgtatgta ataacacaat agccagcaca
     8461 agatttgaca aaattatcta aaacccagcc ctgtacgcct aatgtcgcgt tctcatcagg
     8521 atagtggagt ttaaggtagc caagaatacc gtgatatttg cttagtatgc tagctaatgt
     8581 gtcattaggg ataaattcaa tggcaccttc ttgtggtcct gttgccaaaa ttttgtatgg
     8641 tgtcaatttc aagtccacgt tttcgttttt taataactca ttcattaaac ttatgatctg
     8701 tactaccagt tgatcttgtc tcaaatcatc gccaacctta aacattaagt gatatggttg
     8761 gtttaaggtc gttttgaatg ttatttttag tggcgataag gagcttttaa ataccttcga
     8821 agtttcagga caaacgtcgc atatcaacac atcagggtct agtggtaaag caattggtcg
     8881 tactttgaca agaggcctga ctttcgtctc caataaatgc actagtaatt ccatcttttt
     8941 ggctgtagta tctttcagcc tcttaattgt ttcacaacat tccctgagca catttattag
     9001 cctgacttga tcgttcagta tattccgaga ttttttatcc agtctactcc aaaaggaact
     9061 taaaatctgg tctaaatatg gcttgtcctc agactcggac tttaaatacc aatagaagaa
     9121 acttcctaac cttgggttga ccagtgctct cctaatcaaa aactcagcca agggagaaat
     9181 gacgattggt agcgattctg tcccggaagt ttccgattca ctcgagatgg actttaataa
     9241 tttttggttg gcatgcgatg tagatagtaa catagaatca cccgaaagct tttgcgaaga
     9301 tacggcatcc acaatagtga attcactgtt agatttgtcg gaaaaagtgg acaggttctc
     9361 aaaacagaca gcttctacca actgtaataa gtacaattcc agctccttgt ctgatgcctt
     9421 cttcagccta tttacggcgt acgatctcac tgaaaggttt ttaaaagttg agcctaatag
     9481 ttctaatgcg tcgtctatat caatttctgc ccatgagtcc attagttcta aaacctctac
     9541 tctttccgat tcttctctca aatttgtact ctgtaataac tttgtaagag cttttttatt
     9601 gttcatcaaa taatacctat atttccatat tgaacccttt tcatgtgctg ttaatttagt
     9661 gccagggggg tagttgatga ttttattcaa gtagtccctc tttttaatgt ccggcttcac
     9721 ttgcttgtcc aagtttgcat ttttagatgc tctttccaat ctcctatact tttcctctat
     9781 tggatcattg tttggttggt caggatcgta aaacttcaat gtggagtgat atttgtcccc
     9841 caaagaaatt ttgatttgag ggtcattcct attcggttct cgtaagcctg tgcttagtcc
     9901 aggattgttc ttcaaagttg gaatattcat ttgagtattc ataatttccc tttcgatgaa
     9961 cacaacagga agctctaaca ttggaaattc taagtttaag acaaaggtgc cctgaggcca
    10021 agtcctattt tctcgttgtt tccttaattt gcttatagtg atttcgtcaa gccaagggag
    10081 tctcgtgaat tcaccctgaa aatatttatt caaattttct tgatctttgt tatcagtgac
    10141 cacttcacag tggtcaatca catcataacg aaattttaag gattcaaacc ctctttttaa
    10201 agtacagtct tttaagttaa agatgctcgt ttctagattg aaaaagggaa tttgcttgga
    10261 accattgtat tcccataaaa taatgcgtaa atgactgcta aaggtaagct gtttgatacg
    10321 tatgggcaaa gtcaaccaat aatcccaggt tctagagttt ctgaaaggaa tatacggcgt
    10381 gtatatcgga agagttaaat ttctgtttct ctctttatca aatacctgta aggacacaat
    10441 tagatcacta gaagggaata catttgaccc tatcagcatt aattcagggt tcaggatttt
    10501 ttgagatggc ttcaacagtg gcttatgtcc ttccaatgac ttgattttca ctttcagggg
    10561 aacatccaga tcttgtgaga cacagaatgt gatgttgttc agagccatgg tttatttatg
    10621 tgtgtttatt cgaaactaag ttcttggtgt tttaaaacta aaaaaaagac taactataaa
    10681 agtagaattc aagaagttta agaaatagat ttacagaatt acaatcaata cctaccgtct
    10741 ttatatactt attagtcaag taggggaata atttcaggga actggtttaa accttttttt
    10801 tcagtcgaga tatctggttg ttaatttgat tcgttcattt gaggtttgtg gggccaggtt
    10861 actgccaatt ttcctcttca taaccataaa agctagtatt gtagaatctt tattgttcgg
    10921 agcagtgcgg cgcgaggcac atctgcgttt caggaacgcg accggtgaag acgaggacgc
    10981 acggaggaga gtcttccttc ggagggctgt cacccgctcg gcggcttcta atccgtacgc
    11041 atgctttttt tggatctttg caaagatccg gccggccggt ccggggccgc cagatcgatc
    11101 ttcgagggga attctcttag gattcgattc acattcatct ttttttagct actaatagga
    11161 taaattatag gaatttataa caaattgaaa tatggcaggc agcaaaatta aaattgtctt
    11221 agttttttag tgtatagaag tgaatagcta tataaagtat gtgtaaagtt ggtaacggaa
    11281 cgaaaaatag aaaaggatat tacatgggaa aacatgttgt ttacggagaa atgaaaagta
    11341 tattgtattt tgtacgagct aaaagtacag tgggaacaaa gtcgacttaa gcttgggtta
    11401 ggatccgttt ccgctgatgg tcttatcatt gtgattcact gtagggctta aggtaattga
    11461 cttggatagt aaattggaag aaaacgctaa caaccacttt acattggtta ataaaaattt
    11521 catagccgtt gtccattcaa ggttgggact gctaccatgc aatttgacgg ataatccatt
    11581 aatcttgtcc ttattcatga tatagggtag ctcgagaatg gaagtactat tttcaacgtc
    11641 atttgcgtta ttcattgaag attcatcttg gcttgttgcg agtgtttgag atgaatatga
    11701 tgacgctatc gtagaaagct gccgagtgat ttcgcttatt atttctaatg ttgtttctaa
    11761 tgatttatca aacttcgttt ccttgcggaa gattctcccc aaattgaaat tctcatcata
    11821 gtagacaggc agaatcaacc aatcaccggg agcgttcgta gtggaattat tatattcgac
    11881 actgttaacc attcttttct tgattttgga aaatgaaccc atcggttgta attcgtagtc
    11941 cactaaattt atcttcaggt ttttgtttat ggtagccagt aaaagaatca attggcccag
    12001 tgctgcattg atttccttcc aaggaaccac tgattctggt atactgccta atctcaatcc
    12061 atttatagtg gcaaaggggc cgctatgtga gattttgaat gtagcattga aaatattgat
    12121 tttcctcaat ttgtctaatt gattcaagga taattcgtat tgcagtttta atgactgtaa
    12181 atttttgttg aattgaatgt tatttaaatc cattaaattc tgatcactca gtttttggag
    12241 tttctcattt tccagttgaa ctttcttttc ttgtaaacga actagttctc catctaagtc
    12301 atcatctgtc atttccaaac gtaataattg atccaagagt ctttcctctt cctttttcaa
    12361 attttctttt tccgaaagat tatgggaata ctgcttttct ttattgcttt ctgatatttc
    12421 tttgttttga ctctctaact tcgataaaaa ttgagcgtaa gtatctctct cttttattgc
    12481 atcatcatac tcactcttta gtctgtttat caagatgtta cagcagtcct gacagatagg
    12541 aaagtctatg tttgtttgag aagatagaat gttaaataca ttagtcattg cgttgacttg
    12601 tgtggacaaa gtcttggaat tcaattgtat ttgttggttt tcgtcttcat ccgtagcttc
    12661 ttgttcctct tcgtcatgat ctcgaaaggt actggttcca tcggaagtat cactattagc
    12721 ttttttatta tcatttccgt tggcatttcc ataacgctgg tcctctctgc tattactcgt
    12781 aatattagca ttatcatcat catcgtgatt aagaaaaaca aacgaatcag tgatcaattt
    12841 gtcatctatt aaattcaagt cttttatgtt ctgaatctcc cctaatcgcc tcagcctctc
    12901 cttcggtatt tgaggcccac aattatctgc ggcttcgatg cccttgttgc tgatgacatt
    12961 ttcattcgtt gcagtgataa ttgaattatt tgaaagtagc aaatttcttt gggttaaact
    13021 aagaccttct aaagatgggt ccagttgcag cggcaggtga catgtttggc acttcatgcc
    13081 catggcttcg tcgtgttgag ccaaagcagc agtcttcaat tcaccctcga gtaactcacc
    13141 ttggaagtac aagttttcag aagcagtggt cttcaactcg ccttgaaagt acaaattttc
    13201 agaggcagta gttggtctag aagatccgcc tccctggaaa tacaggtttt ccgcgtctac
    13261 tttcggcgcc tgagcatcat ttagcttttt agcttctgct aaaaggttag cgctttggct
    13321 tgggtcatct tttaaacttt ggatgaaggc gtttcgttgt tcttcgttta agttaggtaa
    13381 atgtaagatc tcatagaacg cgttttgttg ttctttgttg aatttgttgt ctactttcgg
    13441 cgcctgagca tcatttagct ttttagcttc tgctaaaagg ttagcgcttt ggcttgggtc
    13501 atcttttaaa ctttggatga aggcgtttcg ttgttcttcg tttaagttag gtaaatgtaa
    13561 gatctcatag aacgcgtttt gttgttcttt gttgaatttg ttgtctacgg cttcatcgtg
    13621 ggcgccctgg aagtacaggt tctcgctagc acccgcccct gctcccatca tggtttattt
    13681 atgtgtgttt attcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa gactaactat
    13741 aaaagtagaa ttcaagaagt ttaagaaata gatttacaga attacaatca atacctaccg
    13801 tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt taaacctttt
    13861 ttttcagtcg agatatctgg ttgttaattt gattcgttca tttgaggttt gtggggccag
    13921 gttactgcca attttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt
    13981 cggagcagtg cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga
    14041 cgcacggagg agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta
    14101 cgcatgcttt ttttggatct ttgcaaagat ccgcggccct taattaaggc gcgcctgcag
    14161 gacctggtgg cctatatggc caaagagcct gcttttttgt acaaacttgt gatgatccgc
    14221 gcccgatggt gggacggggg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
    14281 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    14341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    14401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    14461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    14521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    14581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    14641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    14701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    14761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    14821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    14881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    14941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    15001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    15061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    15121 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    15181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    15241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    15301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    15361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    15421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    15481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    15541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    15601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    15661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    15721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    15781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    15841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    15901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    15961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    16021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    16081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    16141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    16201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    16261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    16321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    16381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    16441 catttccccg aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa
    16501 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
    16561 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac
    16621 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
    16681 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
    16741 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
    16801 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
    16861 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
    16921 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
    16981 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
    17041 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
    17101 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
    17161 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
    17221 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
    17281 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
    17341 atattttagt agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag
    17401 agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt
    17461 cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct
    17521 gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat
    17581 atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta
    17641 tttatgtagg atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg
    17701 tatcgtatgc ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt
    17761 ggattagtct catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc
    17821 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
