LOCUS       sequence-472856-       20852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human VPS34 complex II in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20852)
  AUTHORS   Mercer TJ, Ohashi Y, Boeing S, Jefferies HBJ, De Tito S, Flynn H, 
            Tremel S, Zhang W, Wirth M, Frith D, Snijders AP, Williams RL, Tooze
            SA
  TITLE     Phosphoproteomic identification of ULK substrates reveals 
            VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy.
  JOURNAL   EMBO J. 2021 Jul 15;40(14):e105985. doi: 10.15252/embj.2020105985. 
            Epub 2021 Jun 14.
  PUBMED    34121209
REFERENCE   2  (bases 1 to 20852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-07-15"; volume: "40(14):e105985. doi"; pages: " 
            10.15252/embj.2020105985. Epub 2021 Jun 14"
COMMENT     Sequence Label: pMO14
FEATURES             Location/Qualifiers
     source          1..20852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        252..631
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        632..835
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    837..855
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    858..876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3110..3133
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3137..3160
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3197..3220
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3242..3262
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3284..3304
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3783..4007
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4075..4454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4455..4658
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4703..4722
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4703..4721
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4817..4819,4820..4822,4823..5536)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4817..4819,4820..4822,4823..5533)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4862..4883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5123..5142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5470..5491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5546..5564)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5547..5564)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    5590..5814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5856..6235
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6236..6439
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    6441..6459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6462..6480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    8007..8231
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8273..8652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8653..8856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    8858..8876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8879..8897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    11735..11959
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        12001..12380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12381..12584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    12586..12604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12607..12625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    16870..17094
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      17140..17568
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17227..17246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17437..17458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(17577..17597)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        17582..17911
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      17762..17897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     17824..17843
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        17959..18006
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             18025..18399
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    18529..18650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(18566..18585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     18620..18639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(18699..18715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18699..18715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(18712..18734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    18723..18739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(18747..18753,18754..18771,18772..18777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18792..18813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(18930..18947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(19101..19689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19181..19200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19860..20651,20652..20720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     20483..20502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20721..20825)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagatg tacgggccag
      241 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      301 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      361 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      421 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      481 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      541 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      601 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      661 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      721 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      781 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
      841 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
      901 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      961 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccatga gcgcctccgc
     1021 gtcggtcggg ggccaggtcc cccagccacc cccgggcccg gccgctgctc tgcctcccgg
     1081 ttctgccgcg cgggccctgc atgtggagct gccgtctcag cagcggcgtc ttcgacatct
     1141 tcggaacatt gctgcccgga acattgttaa tagaaatggc catcagctcc ttgataccta
     1201 ctttacactt cacttgtgta gtactgaaaa gatatataaa gaattttata gaagtgaagt
     1261 gattaagaat tccttgaatc ccacgtggcg aagtctcgat tttggaatta tgccagaccg
     1321 tcttgataca tctgtgtctt gtttcgtggt gaagatatgg ggtggaaagg agaacatcta
     1381 ccagctgttg attgaatgga aagtctgttt ggatgggctg aaatacttgg gtcagcagat
     1441 tcatgcccga aaccaaaatg aaataatttt tgggctgaat gatggatact atggtgctcc
     1501 atttgaacat aagggttatt caaatgctca gaagactatt cttctgcagg tggatcagaa
     1561 ctgtgttcgc aattcttacg atgtcttctc tttgctacgg cttcatagag cccagtgtgc
     1621 aattaaacag actcaggtaa ctgttcagaa aattggaaag gaaattgaag aaaaactaag
     1681 actcacatct acaagcaatg aactgaaaaa aaaaagtgaa tgcctgcagt taaaaatttt
     1741 ggtgcttcag aatgaactgg aacggcagaa gaaagctttg ggacgggagg tggcattact
     1801 gcataagcaa caaattgcat tacaagacaa aggaagtgca ttttcagctg agcacctcaa
     1861 acttcaactc cagaaggaat ccctaaatga gctgaggaag gagtgcactg caaaaagaga
     1921 actcttcttg aagactaatg ctcagttgac aattcgttgc aggcagttac tctctgagct
     1981 ttcctacatt taccctattg atttgaatga acataaggat tactttgtat gcggtgtcaa
     2041 gttgcctaat tctgaggact tccaagcaaa agatgatgga agcattgctg ttgcccttgg
     2101 ttatactgca catctggtct ccatgatttc ctttttccta caagtgcccc tcagatatcc
     2161 tataattcat aaggggtcta gatcaacaat caaagacaat atcaatgaca aactgacgga
     2221 aaaggagaga gagtttccac tgtatccaaa aggaggggag aagttgcagt ttgattatgg
     2281 tgtctatctt ctgaacaaaa atatagcaca gctaagatat caacatggac tagggactcc
     2341 agacttgcgg caaacccttc ccaacctgaa aaacttcatg gagcatggac taatggtcag
     2401 gtgtgacaga catcacacct ccagtgcaat ccctgttcct aagagacaaa gctccatatt
     2461 tgggggtgca gatgtaggct tctctggggg gatcccttca ccagacaaag gacatcgaaa
     2521 acgggccagc tctgagaatg agagacttca gtacaaaacc cctcctccca gttacaactc
     2581 agcattagcc cagcctgtga ccaccgtccc ctccatggga gagaccgaga gaaagataac
     2641 atctctatcc tcctccttgg atacctcctt ggacttctcc aaagaaaaca agaaaaaagg
     2701 agaggatcta gttggcagct taaacggagg ccacgcgaat gtgcacccta gccaagaaca
     2761 aggagaagcc ctctccgggc accgggccac agtcaatggc actctcctac ccagcgagca
     2821 ggccgggtcc gccagtgtcc agcttccagg cgagttccac ccagtctcag aagctgagct
     2881 ctgctgtact gtggagcaag cagaagaaat catcgggctg gaagccacag gtttcgcctc
     2941 aggtgatcag ctagaagcat ttaactgcat cccagtggac agtgctgtgg cagtagagtg
     3001 tgacgaacaa gttctgggag aatttgaaga gttctcccga aggatctatg cactgaatga
     3061 aaacgtatcc agcttccgcc ggccgcgcag gagttccgat aagagatccc tggaagttct
     3121 gttccagggg ccaggctgga gccacccgca gttcgaaaaa ggttctgcgg gatcagctgc
     3181 cggatcggga gcgggttgga gccacccgca gttcgagaaa tctagaccaa ctactgcctc
     3241 tgaaaatttg tactttcaag gcgagttgaa gaccactgct tctgaaaact tgtacttcca
     3301 aggtgagtta ctcgagggtg aattgaagac tgctgctttg gctcaacacg acgaagctgt
     3361 tgataataag ttcaataagg aacagcaaaa cgcattctac gagattttgc atttgccaaa
     3421 tttgaacgag gagcaacgta acgcttttat tcaatccttg aaggatgacc catcccaatc
     3481 tgccaacttg ttagctgaag ccaagaagtt aaacgacgct caagccccaa aagttgacaa
     3541 taagtttaac aaggaacaac aaaacgcctt ttacgaaatt ttgcacttgc caaacttgaa
     3601 cgaagaacaa agaaacgctt tcattcaatc tttgaaggac gacccatctc aatctgctaa
     3661 tttgttggct gaagctaaga agttgaacgg tgctcaagct ccaaaggttg acgctaactc
     3721 tgctggtaag tctacttgag tcgagtctag agggcccgtt taaacccgct gatcagcctc
     3781 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3841 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3901 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3961 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4021 aagaaccagc tggggctcta ggcgcgttga cattgattat tgactasccg cgttgacatt
     4081 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4141 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4201 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4261 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4321 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4381 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4441 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     4501 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     4561 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     4621 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
     4681 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
     4741 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
     4801 ctggcggccg ctcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4861 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4921 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4981 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5041 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5101 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5161 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5221 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5281 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5341 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5401 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5461 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5521 cgagctgtac aagtaagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     5581 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5641 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5701 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5761 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5821 aggcggaaag aaccagctgg ggctctaggc gcgttgacat tgattattga ctagttatta
     5881 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     5941 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     6001 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     6061 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     6121 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     6181 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     6241 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     6301 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     6361 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     6421 ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga
     6481 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     6541 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa
     6601 gcttggtacc atggaagggt ctaagacgtc caacaacagc accatgcagg tgagcttcgt
     6661 gtgccagcgc tgcagccagc ccctgaaact ggacacgagt ttcaagatcc tggaccgtgt
     6721 caccatccag gaactcacag ctccattact taccacagcc caggcgaaac caggagagac
     6781 ccaggaggaa gagactaact caggagagga gccatttatt gaaactcctc gccaggatgg
     6841 tgtctctcgc agattcatcc ccccagccag gatgatgtcc acagaaagtg ccaacagctt
     6901 cactctgatt ggggaggcat ctgatggcgg caccatggag aacctcagcc gaagactgaa
     6961 ggtcactggg gacctttttg acatcatgtc gggccagaca gatgtggatc acccactctg
     7021 tgaggaatgc acagatactc ttttagacca gctggacact cagctcaacg tcactgaaaa
     7081 tgagtgtcag aactacaaac gctgtttgga gatcttagag caaatgaatg aggatgacag
     7141 tgaacagtta cagatggagc taaaggagct ggcactagag gaggagaggc tgatccagga
     7201 gctggaagac gtggaaaaga accgcaagat agtggcagaa aatctcgaga aggtccaggc
     7261 tgaggctgag agactggatc aggaggaagc tcagtatcag agagaataca gtgaatttaa
     7321 acgacagcag ctggagctgg atgatgagct gaagagtgtt gaaaaccaga tgcgttatgc
     7381 ccagacgcag ctggataagc tgaagaaaac caacgtcttt aatgcaacct tccacatctg
     7441 gcacagtgga cagtttggca caatcaataa cttcaggctg ggtcgcctgc ccagtgttcc
     7501 cgtggaatgg aatgagatta atgctgcttg gggccagact gtgttgctgc tccatgctct
     7561 ggccaataag atgggtctga aatttcagag ataccgactt gttccttacg gaaaccattc
     7621 atatctggag tctctgacag acaaatctaa ggagctgccg ttatactgtt ctggggggtt
     7681 gcggtttttc tgggacaaca agtttgacca tgcaatggtg gctttcctgg actgtgtgca
     7741 gcagttcaaa gaagaggttg agaaaggcga gacacgtttt tgtcttccct acaggatgga
     7801 tgtggagaaa ggcaagattg aagacacagg aggcagtggc ggctcctatt ccatcaaaac
     7861 ccagtttaac tctgaggagc agtggacaaa agctctcaag ttcatgctga cgaatctgaa
     7921 gtggggtctg gcttgggtgt cctctcaatt ttataacaaa tgagtcgagt ctagagggcc
     7981 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8041 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8101 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8161 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8221 gggctctatg gcttctgagg cggaaagaac cagctggggc tctaggcgcg ttgacattga
     8281 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     8341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     8581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     8641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     8701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     8761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     8821 aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc
     8881 cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga
     8941 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
     9001 ctagcgttta aacttaagct tggtaccatg ggggaagcag agaagtttca ctacatctat
     9061 agttgtgacc tggatatcaa cgtccagctt aagataggaa gcttggaagg gaagagagaa
     9121 caaaagagtt ataaagctgt cctggaagac ccaatgttga agttctcagg actatatcaa
     9181 gagacatgct ctgatcttta tgttacttgt caagtttttg cagaagggaa gcctttggcc
     9241 ttgccagtga gaacatccta caaagcattt agtacaagat ggaactggaa tgaatggctg
     9301 aaactaccag taaaataccc tgacctgccc aggaatgccc aagtggccct caccatctgg
     9361 gatgtgtatg gtcccggaaa agcagtgcct gtaggaggaa caacggtttc gctctttgga
     9421 aaatacggca tgtttcgcca agggatgcat gacttgaaag tctggcctaa tgtagaagca
     9481 gatggatcag aacccacaaa aactcctggc agaacaagta gcactctctc agaagatcag
     9541 atgagccgtc ttgccaagct caccaaagct catcgacaag gacacatggt gaaagtagat
     9601 tggctggata gattgacatt tagagaaata gaaatgataa atgagagtga aaaacgaagt
     9661 tctaatttca tgtacctgat ggttgaattt cgatgtgtca agtgtgatga taaggaatat
     9721 ggtattgttt attatgaaaa ggacggtgat gaatcatctc caattttaac aagttttgaa
     9781 ttagtgaaag ttcctgaccc ccagatgtct atggagaatt tagttgagag caaacaccac
     9841 aagcttgccc ggagtttaag aagtggacct tctgaccacg atctgaaacc caatgctgcc
     9901 acgagagatc agttaaatat tattgtgagt tatccaccaa ccaagcaact tacctatgaa
     9961 gaacaagatc ttgtttggaa gtttagatat tatcttacga atcaagaaaa agccttgaca
    10021 aaattcttga aatgtgttaa ttgggatcta cctcaagagg ccaaacaggc cttggaactt
    10081 ctgggaaaat ggaagccgat ggatgtagag gactccttgg agctgttatc ctctcattac
    10141 accaacccaa ctgtgaggcg ttatgctgtt gcccggttgc gacaggccga tgatgaggat
    10201 ttgttgatgt acctattaca attggtccag gctctcaaat atgaaaattt tgatgatata
    10261 aagaatggat tggaacctac caagaaggat agtcagagtt cagtgtcaga aaatgtgtca
    10321 aattctggaa taaattctgc agaaatagat agctcccaaa ttataaccag cccccttcct
    10381 tcagtctctt cacctcctcc tgcatcaaaa acaaaagaag ttccagatgg cgaaaatctg
    10441 gaacaagatc tctgtacctt cttgatatcg agagcctgca aaaactcaac actggctaat
    10501 tatttatact ggtatgtgat agtggaatgt gaagatcaag atactcagca gagagatcca
    10561 aagacccatg agatgtactt gaacgtaatg agaagattca gccaagcatt gttgaagggt
    10621 gataagtctg tcagagttat gcgttctttg ctggctgcac aacagacatt tgtagatcgg
    10681 ttggtgcatc taatgaaggc agtacaacgc gaaagtggaa atcgtaagaa aaagaatgag
    10741 agactacagg cattgcttgg agataatgaa aagatgaatt tgtcagatgt ggaacttatc
    10801 ccgttgcctt tagaacccca agtgaaaatt agaggaataa ttccggaaac agctacactg
    10861 tttaaaagtg cccttatgcc tgcacagttg ttttttaaga cggaagatgg aggcaaatat
    10921 ccagttatat ttaagcatgg agatgattta cgtcaagatc aacttattct tcaaatcatt
    10981 tcactcatgg acaagctgtt acggaaagaa aatctggact tgaaattgac accttataag
    11041 gtgttagcca ccagtacaaa acatggcttc atgcagttta tccagtcagt tcctgtggct
    11101 gaagttcttg atacagaggg aagcattcag aactttttta gaaaatatgc accaagtgag
    11161 aatgggccaa atgggattag tgctgaggtc atggacactt acgttaaaag ctgtgctgga
    11221 tattgcgtga tcacctatat acttggagtt ggagacaggc acctggataa ccttttgcta
    11281 acaaaaacag gcaaactctt ccacatagac tttggatata ttctgggtcg tgatccaaag
    11341 cctcttcctc caccaatgaa gctgaataaa gaaatggtag aaggaatggg gggcacacag
    11401 agtgagcagt accaagagtt ccgtaaacag tgttacacgg ctttcctcca cctgcgaagg
    11461 tattctaatc tgattttgaa cttgttttcc ttgatggttg atgcaaacat tccagatatt
    11521 gcacttgaac cagataaaac tgtgaaaaag gttcaggata aattccgctt agacctgtcg
    11581 gatgaagagg ctgtgcatta catgcagagt ctgattgatg agagtgtcca tgctcttttt
    11641 gctgcagtgg tggaacagat tcacaagttt gcccagtact ggcgcaaatg agtcgagtct
    11701 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
    11761 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    11821 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    11881 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
    11941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc taggcgcgtt
    12001 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    12061 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
    12121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
    12181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
    12241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
    12301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    12361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
    12421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
    12481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
    12541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
    12601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
    12661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
    12721 tccggactct agcgtttaaa cttaagctta tgggaaatca gcttgctggc attgctccct
    12781 cccagatcct ttctgtagag agttattttt cagatattca tgactttgaa tatgataaaa
    12841 gcctggggag tactcggttt tttaaagttg ctcgagccaa gcaccgagaa ggcctggtcg
    12901 ttgtgaaggt ttttgcaatt caggatccca cattgccttt aaccagctat aaacaagagc
    12961 tggaggaact gaaaatcagg cttaattctg cacagaattg tctacctttc cagaaagcat
    13021 cagaaaaagc atctgagaaa gcagctatgc tctttaggca gtatgtgcga gacaatctct
    13081 atgatcgcat cagtacccgt ccattcttga ataacattga gaagcgctgg attgctttcc
    13141 agatcctgac agctgtggac caagcacaca aatctggagt tcgtcatggg gacatcaaga
    13201 ctgagaatgt gatggtcacc agttggaatt gggttcttct aactgatttt gccagtttta
    13261 agcccactta tcttccagaa gacaacccgg cagatttcaa ttatttcttt gacacatcac
    13321 ggaggagaac ttgctatatt gctcctgaac gttttgttga tggtgggatg tttgccactg
    13381 agttagaata tatgagagat ccttcaactc cgcttgtaga cttaaatagc aatcagagaa
    13441 caagaggaga gttgaagaga gcaatggaca tcttttcagc aggttgtgtg atagctgagc
    13501 tttttacaga aggtgtacca ttatttgatc tctctcaact tttggcttat agaaatggac
    13561 attttttccc tgaacaagtg ctaaataaaa ttgaagatca cagtatcaga gaattggtaa
    13621 ctcagatgat tcaccgtgag ccagataaac gtttagaggc agaagattac ttaaaacagc
    13681 agcgtggcaa tgcctttcct gaaatatttt acacttttct tcagccctac atggcccagt
    13741 ttgccaagga aacgtttctt tctgcagatg agcgtattct ggttatacgg aaggatttgg
    13801 gcaacattat tcacaatctc tgtggacatg atctgccaga aaaagccgaa ggagagccta
    13861 aggaaaatgg gctggttatc ttggtatctg ttataacatc ctgcctacag acccttaaat
    13921 actgtgattc caaactagct gctttggaac tgattcttca tttggctcca agattaagtg
    13981 ttgaaatcct tttggatcgt attactccat atcttttgca tttcagcaat gactctgttc
    14041 ctagggtgag ggctgaagcc ttgaggacgt tgaccaaagt tcttgctctc gtcaaagagg
    14101 ttcctcgtaa tgatatcaat atttatccgg aatacattct gccaggcata gcccacttag
    14161 cccaagatga tgctactatc gttagactag cctatgctga aaacatagct ctgctggcag
    14221 aaacagctct gagattcctg gaattagtac agttaaaaaa tcttaatatg gaaaatgacc
    14281 ccaataatga agaaatagat gaggttacac atccaaatgg aaattatgac acagagctgc
    14341 aagccttaca tgaaatggtc cagcagaaag ttgttacttt gctaagtgac cctgaaaata
    14401 ttgtaaaaca aaccttgatg gaaaatggaa taacacggct gtgtgtattc tttggacgtc
    14461 agaaagccaa cgatgttttg ttgtcccaca tgattacttt cctaaatgat aagaatgatt
    14521 ggcatctacg tggagcattt tttgatagta tagttggtgt tgctgcctat gttggctggc
    14581 aaagctcctc aattctcaag cctctgctgc aacaaggtct tagtgatgct gaggaatttg
    14641 tcattgtgaa agctctttat gcccttactt gtatgtgcca gttaggactg ctacaaaaac
    14701 cccatgttta cgaatttgcc agtgatattg cccccttcct gtgtcatccc aatttatgga
    14761 tacgttatgg tgccgtggga tttatcacag tggtagctcg tcaaataagt acagctgatg
    14821 tctactgtaa actgatgcct tatcttgacc catatattac ccaaccaata atacagattg
    14881 aaagaaaact tgttctgctc agtgttttaa aggaaccagt aagtcgttct atatttgatt
    14941 atgctttgag gtctaaagat attactagct tgttcagaca tcttcacatg cgtcagaaga
    15001 aacgaaatgg ttctcttccc gactgccctc cgccagagga tcctgccata gcacagcttc
    15061 tgaagaagtt gctctcacag ggaatgacag aggaagagga agacaaactt ctggcactga
    15121 aagacttcat gatgaaatct aataaagcaa aggccaatat agtggaccag agccatcttc
    15181 atgatagtag tcagaaaggt gtaattgact tggcagcttt aggcataact gggagacaag
    15241 ttgatcttgt taaaaccaaa caagaaccag atgacaaacg ggccagaaaa catgtaaaac
    15301 aagactcaaa tgtaaatgaa gaatggaaaa gcatgtttgg gtcactggac ccaccaaaca
    15361 tgccacaggc cctacctaaa gggagtgatc aggaggtgat tcagactggg aaacctcctc
    15421 gttccgagtc ctctgctggc atttgtgtcc ctttgtcaac ttcttcacag gttccagaag
    15481 tgacaactgt ccaaaataaa aaaccagtaa taccggtttt aagtagtaca atcttaccat
    15541 ccacctatca gattcgaatt acaacttgta aaactgaact tcagcaactc atccagcaaa
    15601 agcgggagca gtgcaatgct gagagaatag ctaagcagat gatggaaaat gctgaatggg
    15661 agagtaaacc accaccacct ggatggcgtc ctaaagggct gttagttgcc catcttcatg
    15721 agcataaatc tgctgtgaat cgaattagag tctctgatga acactcactt tttgcaacat
    15781 gttcaaatga tggcacagtg aaaatctgga acagtcaaaa gatggagggg aagaccacca
    15841 ctaccagatc tattcttaca tacagccgaa ttggaggacg agtcaagacg ctcacattct
    15901 gccaaggctc ccactattta gccatagcat ctgataatgg tgctgtccag cttcttggaa
    15961 ttgaggcttc taagctgccc aagtctccta aaatccatcc tctacaaagc agaattctag
    16021 atcagaagga ggacggttgt gttgtggata tgcatcactt caactctgga gcacagtctg
    16081 ttcttgccta tgccactgtg aatggctctc tggttggctg ggaccttagg tcttcaagca
    16141 atgcgtggac tttaaagcat gatttaaagt cgggcctcat cacttccttt gctgtggaca
    16201 tccaccaatg ctggctctgc attggtacaa gcagtggcac catggcttgt tgggacatga
    16261 ggttccagtt gccaatttca agtcactgtc atccttccag ggctcgaatc agacgcctct
    16321 caatgcaccc tctgtatcag tcctgggtga ttgcagctgt tcagggcaac aacgaagtgt
    16381 ccatgtggga catggagact ggtgacagaa gatttactct ctgggccagc agtgcaccac
    16441 cactttctga attacagcct tctcctcata gcgtccatgg tatctactgt agtcctgcag
    16501 atggaaatcc tatcctacta acagctggct cagatatgaa aataaggttt tgggacttgg
    16561 cttacccaga aaggtcctat gttgttgcag gaagtactag ttccccatct gtgtcctact
    16621 acaggaaaat aattgaaggc actgaagttg tccaggaaat tcagaataag cagaaagtag
    16681 gaccaagtga tgacacccct cgaaggggcc cagagtccct gcccgtggga catcatgaca
    16741 tcatcactga tgtcgccaca ttccagacca cacagggctt catcgtaact gcttctagag
    16801 atgggattgt gaaggtgtgg aaataagtcg agtctagagg gcccgtttaa acccgctgat
    16861 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
    16921 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
    16981 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
    17041 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
    17101 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
    17161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    17221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    17281 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    17341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    17401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    17461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    17521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
    17581 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    17641 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    17701 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    17761 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    17821 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
    17881 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
    17941 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
    18001 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
    18061 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
    18121 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
    18181 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
    18241 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
    18301 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
    18361 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
    18421 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    18481 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    18541 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    18601 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    18661 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    18721 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    18781 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    18841 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    18901 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    18961 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    19021 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    19081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    19141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    19201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    19261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    19321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    19381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    19441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    19501 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    19561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    19621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    19681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    19741 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    19801 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    19861 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    19921 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    19981 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    20041 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    20101 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    20161 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    20221 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    20281 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    20341 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    20401 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    20461 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    20521 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    20581 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    20641 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    20701 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    20761 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    20821 ccgcgcacat ttccccgaaa agtgccacct ga
//
