LOCUS       sequence-472868-        5137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  over-express E. coli SSB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   O'Donnell Lab
  TITLE     O'Donnell Lab Batch 1
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Ec ssb-pET(3d)
FEATURES             Location/Qualifiers
     source          1..5137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             921..1112
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1106..1128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1214..1353
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1269..1288
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1368..1385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1539..2127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1619..1638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2298..3089,3090..3158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2921..2940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3159..3263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3331..3349
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3376..3381,3382..3398,3399..3404)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(3770..3817)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     3813..3831
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     RBS             4425..4447
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(4478..4497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4479..4497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4581..4600)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
       61 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
      121 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
      181 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
      241 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
      301 tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
      361 atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
      421 cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
      481 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
      541 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
      601 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
      661 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
      721 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
      781 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
      841 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
      901 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
      961 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     1021 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     1081 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     1141 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     1201 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     1261 taactatgcg gcatcagagc agattgtact gagagtgcac cattgcggtg tgaaataccg
     1321 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     1381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2581 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     2641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2881 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     2941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     3301 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     3361 caagaattct catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca
     3421 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
     3481 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
     3541 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
     3601 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
     3661 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
     3721 ggcgaccaca cccgtcctgt ggatatccgg atatagttcc tcctttcagc aaaaaacccc
     3781 tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag
     3841 ccaactcagc ttcctttcgg gctttgttag cagccggatc ctcagaacgg aatgtcatca
     3901 tcaaagtcca tcggcggctc gttagacggc gctgccggag cggactgctg cgggcgagac
     3961 tgcgcgccgc cgctgaactg attgccaccc tgcggctgct gaggctgacc ccaaccgccc
     4021 tgcggctgac caccaccgat attgccacct gccggagcgc caccaccctg acgaccaccc
     4081 agcatctgca tggtgccgcc aacgttcacc acgacttctg tggtgtagcg atcctgaccg
     4141 gattgatcgg tccatttacg ggtacgcagc tgaccttcga tataaacctg agaaccttta
     4201 cgcagatatt cgctcgccac ttctgccagt ttgccgaaca gcacaacgcg gtgccattca
     4261 gtctgttctt tcatctcgcc ggtcgcttta tcacgccagg attcggaagt agccagcgta
     4321 atgttggcaa ctgcgccacc atttggcatg tagcgtactt ccgggtcctg acccagatta
     4381 ccaacgagaa taaccttgtt tacgcctctg ctggccatgg tatatctcct tcttaaagtt
     4441 aaacaaaatt atttctagag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt
     4501 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     4561 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     4621 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     4681 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     4741 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
     4801 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     4861 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     4921 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     4981 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     5041 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     5101 cgtcttgctg gcgttcgcga cgcgaggctg gatggcc
//
