LOCUS       sequence-47289-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  TITLE     SGC Q3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L3MBTL3 (3UT1)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..174
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             187..207
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcaccatca ccatgaattc
       61 atggacgaaa aaaccaccgg ttggcgtggt ggtcacgttg ttgaaggtct ggctggtgaa
      121 ctggaacagc tgcgtgctcg tctggaacac cacccgcagg gtcagcgtga accgagcagc
      181 ggcagagaaa acttgtattt ccagggcaag aaagcgtggt gctgggcatc ctacctggaa
      241 gaggagaaag cggtggcagt gccggcgaag ctgttcaagg agcatcaatc ctttccatat
      301 aacaaaaatg gattcaaagt tggcatgaaa ttagaaggcg tggatcccga gcatcagtct
      361 gtgtactgtg tcctcaccgt cgcggaggtt tgtggatacc ggataaagct tcactttgat
      421 gggtattctg attgctatga cttctgggtg aatgcagacg ctctggatat ccacccagtt
      481 gggtggtgtg agaaaaccgg ccacaaactc catcctccaa aagggtataa agaagaagaa
      541 ttcaattggc agacctatct taagacatgt aaagctcaag ctgctcctaa gtcattattt
      601 gaaaatcaga atataacagt gatcccatcg ggctttcgag ttggtatgaa gcttgaggca
      661 gtagacaaaa agaatccctc attcatctgt gttgctacgg taacagatat ggtggacaat
      721 cgtttcctgg tacattttga caactgggat gagagctatg actattggtg tgaagcatca
      781 agtccacata ttcatccagt tggttggtgt aaggaacata gaagaaccct tattactcca
      841 ccaggttatc caaatgtgaa acatttttct tgg
//
