LOCUS       sequence-472907-       16396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene,
            RT and Env impaired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   Xu W, Gonepudi NK, Lu M
  TITLE     Protocol for click labeling of HIV-1 envelope on amber-free virions 
            prepared using genetic code expansion.
  JOURNAL   STAR Protoc. 2025 Jul 26;6(3):103995. doi: 
            10.1016/j.xpro.2025.103995.
  PUBMED    40716060
REFERENCE   2  (bases 1 to 16396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2025-07-26"; volume: "6(3):103995. doi"; pages: " 
            10.1016/j.xpro.2025.103995"
COMMENT     Sequence Label: Amber-free pNL4-3 FLuc Env-RT-
FEATURES             Location/Qualifiers
     source          1..16396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(469..486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(640..1228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(720..739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1399..2190,2191..2259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2022..2041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2260..2364)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2432..2450
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2610..2629
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             4322..4579
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             4735..6237
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             6030..9041
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8726..8843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11710..11943
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12128..12172
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             12466..12492
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             12756..14402
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(12819..12837)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     LTR             14592..15225
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tttggtagag gcagcgtttt gccgtgtggc cctggctggt ctcgaactcc tgggctcaag
       61 tgatccagcc tcagcctccc aaagtgctgg gacaaccggg gccagtcact gcacctggcc
      121 ctaaaccata atttctaatc ttttggctaa tttgttagtc ctacaaaggc agtctagtcc
      181 ccaggcaaaa agggggtttg tttcgggaaa gggctgttac tgtctttgtt tcaaactata
      241 aactaagttc ctcctaaact tagttcggcc tacacccagg aatgaacaag gagagcttgg
      301 aggttagaag cacgatggaa ttggttaggt cagatctctt tcactgtctg agttataatt
      361 ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag tcgctgcatt aatgaatcgg
      421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1081 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1321 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1381 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1441 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1501 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1561 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1621 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1681 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1741 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1801 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1861 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1921 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1981 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2041 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2101 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2161 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2221 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2281 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2341 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2401 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2461 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2521 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2581 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2641 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2701 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2761 acgccagggg aggcagagat tgcagtaagc tgagatcgca gcactgcact ccagcctggg
     2821 cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat attccaatct
     2881 tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct tattccagaa
     2941 ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct tcacctgaaa
     3001 tgtgtgtata caaaatctag gccagtccag cagagcctaa aggtaaaaaa taaaataata
     3061 aaaaataaat aaaatctagc tcactccttc acatcaaaat ggagatacag ctgttagcat
     3121 taaataccaa ataacccatc ttgtcctcaa taattttaag cgcctctctc caccacatct
     3181 aactcctgtc aaaggcatgt gccccttccg ggcgctctgc tgtgctgcca accaactggc
     3241 atgtggactc tgcagggtcc ctaactgcca agccccacag tgtgccctga ggctgcccct
     3301 tccttctagc ggctgccccc actcggcttt gctttcccta gtttcagtta cttgcgttca
     3361 gccaaggtct gaaactaggt gcgcacagag cggtaagact gcgagagaaa gagaccagct
     3421 ttacaggggg tttatcacag tgcaccctga cagtcgtcag cctcacaggg ggtttatcac
     3481 attgcaccct gacagtcgtc agcctcacag ggggtttatc acagtgcacc cttacaatca
     3541 ttccatttga ttcacaattt ttttagtctc tactgtgcct aacttgtaag ttaaatttga
     3601 tcagaggtgt gttcccagag gggaaaacag tatatacagg gttcagtact atcgcatttc
     3661 aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt gatgactacc tcagttggat
     3721 ctccacaggt cacagtgaca caagataacc aagacacctc ccaaggctac cacaatgggc
     3781 cgccctccac gtgcacatgg ccggaggaac tgccatgtcg gaggtgcaag cacacctgcg
     3841 catcagagtc cttggtgtgg agggagggac cagcgcagct tccagccatc cacctgatga
     3901 acagaaccta gggaaagccc cagttctact tacaccagga aaggctggaa gggctaattt
     3961 ggtcccaaaa aagacaagag atccttgatc tgtggatcta ccacacacaa ggctacttcc
     4021 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     4081 gcttcaagtt agtaccagtt gaaccagagc aagtagaaga ggccaatgaa ggagagaaca
     4141 acagcttgtt acaccctatg agccagcatg ggatggagga cccggaggga gaagtattag
     4201 tgtggaagtt tgacagcctc ctagcatttc gtcacatggc ccgagagctg catccggagt
     4261 actacaaaga ctgctgacat cgagctttct acaagggact ttccgctggg gactttccag
     4321 ggaggtgtgg cctgggcggg actggggagt ggcgagccct cagatgctac atataagcag
     4381 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4441 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gctcaaagta
     4501 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4561 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gtaaagccag
     4621 aggagatctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4681 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4741 gcgagagcgt cggtattaag cgggggagaa ttagataaat gggaaaaaat tcggttaagg
     4801 ccagggggaa agaaacaata taaactaaaa catatagtat gggcaagcag ggagctagaa
     4861 cgattcgcag ttaatcctgg ccttttagag acatcagaag gctgtagaca aatactggga
     4921 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacaata
     4981 gcagtcctct attgtgtgca tcaaaggata gatgtaaaag acaccaagga agccttagat
     5041 aagatagagg aagagcaaaa caaaagtaag aaaaaggcac agcaagcagc agctgacaca
     5101 ggaaacaaca gccaggtcag ccaaaattac cctatagtgc agaacctcca ggggcaaatg
     5161 gtacatcagg ccatatcacc tagaacttta aatgcatggg taaaagtagt agaagagaag
     5221 gctttcagcc cagaagtaat acccatgttt tcagcattat cagaaggagc caccccacaa
     5281 gatttaaata ccatgctaaa cacagtgggg ggacatcaag cagccatgca aatgttaaaa
     5341 gagaccatca atgaggaagc tgcagaatgg gatagattgc atccagtgca tgcagggcct
     5401 attgcaccag gccagatgag agaaccaagg ggaagtgaca tagcaggaac tactagtacc
     5461 cttcaggaac aaataggatg gatgacacat aatccaccta tcccagtagg agaaatctat
     5521 aaaagatgga taatcctggg attaaataaa atagtaagaa tgtatagccc taccagcatt
     5581 ctggacataa gacaaggacc aaaggaaccc tttagagact atgtagaccg attctataaa
     5641 actctaagag ccgagcaagc ttcacaagag gtaaaaaatt ggatgacaga aaccttgttg
     5701 gtccaaaatg cgaacccaga ttgtaagact attttaaaag cattgggacc aggagcgaca
     5761 ctagaagaaa tgatgacagc atgtcaggga gtggggggac ccggccataa agcaagagtt
     5821 ttggctgaag caatgagcca agtaacaaat ccagctacca taatgataca gaaaggcaat
     5881 tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg gcaaagaagg gcacatagcc
     5941 aaaaattgca gggcccctag gaaaaagggc tgttggaaat gtggaaagga aggacaccaa
     6001 atgaaagatt gtactgagag acaggctaat tttttaggga agatctggcc ttcccacaag
     6061 ggaaggccag ggaattttct tcagagcaga ccagagccaa cagccccacc agaagagagc
     6121 ttcaggtttg gggaagagac aacaactccc tctcagaagc aggagccgat agacaaggaa
     6181 ctgtatcctt tagcttccct cagatcactc tttggcagcg acccctcgtc acaataaaga
     6241 taggggggca attaaaggaa gctctattag atacaggagc agatgataca gtattagaag
     6301 aaatgaattt gccaggaaga tggaaaccaa aaatgatagg gggaattgga ggttttatca
     6361 aagtaagaca gtatgatcag atactcatag aaatctgcgg acataaagct ataggtacag
     6421 tattagtagg acctacacct gtcaacataa ttggaagaaa tctgttgact cagattggct
     6481 gcactttaaa ttttcccatt agtcctattg agactgtacc agtaaaatta aagccaggaa
     6541 tggatggccc aaaagttaaa caatggccat tgacagaaga aaaaataaaa gcattagtag
     6601 aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa aattgggcct gaaaatccat
     6661 acaatactcc agtatttgcc ataaagaaaa aagacagtac taaatggaga aaattagtag
     6721 atttcagaga acttaataag agaactcaag atttctggga agttcaatta ggaataccac
     6781 atcctgcagg gttaaaacag aaaaaatcag taacagtact ggatgtgggc gatgcatatt
     6841 tttcagttcc cttagataaa gacttcagga agtatactgc atttaccata cctagtataa
     6901 acaatgagac accagggatt agatatcagt acaatgtgct tccacaggga tggaaaggat
     6961 caccagcaat attccagtgt agcatgacaa aaatcttaga gccttttaga aaacaaaatc
     7021 cagacatagt catctatcaa tacatggatg atttgtatgt aggatctgac ttagaaatag
     7081 ggcagcatag aacaaaaata gaggaactga gacaacatct gttgaggtgg ggatttacca
     7141 caccagacaa aaaacatcag aaagaacctc cattcctttg gatgggttat gaactccatc
     7201 ctgataaatg gacagtacag cctatagtgc tgccagaaaa ggacagctgg actgtcaatg
     7261 acatacagaa attagtggga aaattgaatt gggcaagtca gatttatgca gggattaaag
     7321 taaggcaatt atgtaaactt cttaggggaa ccaaagcact aacagaagta gtaccactaa
     7381 cagaagaagc agagctagaa ctggcagaaa acagggagat tctaaaagaa ccggtacatg
     7441 gagtgtatta tgacccatca aaagacttaa tagcagaaat acagaagcag gggcaaggcc
     7501 aatggacata tcaaatttat caagagccat ttaaaaatct gaaaacagga aagtatgcaa
     7561 gaatgaaggg tgcccacact aatgatgtga aacaattaac agaggcagta caaaaaatag
     7621 ccacagaaag catagtaata tggggaaaga ctcctaaatt taaattaccc atacaaaagg
     7681 aaacatggga agcatggtgg acagagtatt ggcaagccac ctggattcct gagtgggagt
     7741 ttgtcaatac ccctccctta gtgaagttat ggtaccagtt agagaaagaa cccataatag
     7801 gagcagaaac tttctatgta gatggggcag ccaataggga aactaaatta ggaaaagcag
     7861 gatatgtaac tgacagagga agacaaaaag ttgtccccct aacggacaca acaaatcaga
     7921 agactgagtt acaagcaatt catctagctt tgcaggattc gggattagaa gtaaacatag
     7981 tgacagactc acaatatgca ttgggaatca ttcaagcaca accagataag agtgaatcag
     8041 agttagtcag tcaaataata gagcagttaa taaaaaagga aaaagtctac ctggcatggg
     8101 taccagcaca caaaggaatt ggaggaaatg aacaagtaga taaattggtc agtgctggaa
     8161 tcaggaaagt actattttta gatggaatag ataaggccca agaagaacat gagaaatatc
     8221 acagtaattg gagagcaatg gctagtgatt ttaacctacc acctgtagta gcaaaagaaa
     8281 tagtagccag ctgtgataaa tgtcagctaa aaggggaagc catgcatgga caagtagact
     8341 gtagcccagg aatatggcag ctagattgta cacatttaga aggaaaagtt atcttggtag
     8401 cagttcatgt agccagtgga tatatagaag cagaagtaat tccagcagag acagggcaag
     8461 aaacagcata cttcctctta aaattagcag gaagatggcc agtaaaaaca gtacatacag
     8521 acaatggcag caatttcacc agtactacag ttaaggccgc ctgttggtgg gcggggatca
     8581 agcaggaatt tggcattccc tacaatcccc aaagtcaagg agtaatagaa tctatgaata
     8641 aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt aagacagcag
     8701 tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     8761 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     8821 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     8881 aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa gataatagtg
     8941 acataaaagt agtgccaaga agaaaagcaa agatcatcag ggattatgga aaacagatgg
     9001 caggtgatga ttgtgtggca agtagacagg atgaggatta acacatggaa aagattagta
     9061 aaacaccata tgtatatttc aaggaaagct aaggactggt tttatagaca tcactatgaa
     9121 agtactaatc caaaaataag ttcagaagta cacatcccac taggggatgc taaattagta
     9181 ataacaacat attggggtct gcatacagga gaaagagact ggcatttggg tcagggagtc
     9241 tccatagaat ggaggaaaaa gagatatagc acacaagtag accctgacct agcagaccaa
     9301 ctaattcatc tgcactattt tgattgtttt tcagaatctg ctataagaaa taccatatta
     9361 ggacgtatag ttagtcctag gtgtgaatat caagcaggac ataacaaggt aggatctcta
     9421 cagtacttgg cactagcagc attaataaaa ccaaaacaga taaagccacc tttgcctagt
     9481 gttaggaaac tgacagagga cagatggaac aagccccaga agaccaaggg ccacagaggg
     9541 agccatacaa tgaatggaca ctagagcttt tagaggaact taattaagag tgaagctgtt
     9601 agacattttc ctaggatatg gctccataac ttaggacaac atatctatga aacttacggg
     9661 gatacttggg caggagtgga agccataata agaattctgc aacaactgct gtttatccat
     9721 ttcagaattg ggtgtcgaca tagcagaata ggcgttactc gacagaggag agcaagaaat
     9781 ggagccagta gatcctagac tagagccctg gaagcatcca ggaagtcagc ctaaaactgc
     9841 ttgtaccaat tgctattgta aaaagtgttg ctttcattgc caagtttgtt tcatgacaaa
     9901 agccttaggc atctcctatg gcaggaagaa gcggagacag cgacgaagag ctcatcagaa
     9961 cagtcagact catcaagctt ctctatcaaa gcagtaagta gtacatgtaa tgcaacctat
    10021 aatagtagca atagtagcat tagtagtagc aataataata gcaatagttg tgtggtccat
    10081 agtaatcata gaatatagga aaatattaag acaaagaaaa atagacaggt taattgatag
    10141 actaatagaa agagcagaag acagtggcaa tgagagtgaa ggagaagtat cagcacttgt
    10201 ggagatgggg gtggaaatgg ggcaccatgc tccttgggat attgatgatc tgtagtgcta
    10261 cagaaaaatt gtgggtcaca gtctattatg gggtacctgt gtggaaggaa gcaaccacca
    10321 ctctattttg tgcatcagat gctaaagcat atatgataca gaggtacata atgtttgggc
    10381 cacacatgcc tgtgtaccca cagaccccaa cccacaagaa gtagtattgg taaatgtgac
    10441 agaaaatttt aacatgtgga aaaatgacat ggtagaacag atgcatgagg atataatcag
    10501 tttatgggat caaagcctaa agccatgtgt aaaattaacc ccactctgtg ttagtttaaa
    10561 gtgcactgat ttgaagaatg atactaatac caatagtagt agcgggagat aaatataaga
    10621 gaaaggagag ataaaaaact gctctttcaa tatcagcaca agcataagag ataaggtgca
    10681 gaaagaatat gcattctttt ataaacttga tatagtacca atagataata ccagctatag
    10741 gttgataagt tgtaacacct cagtcattac acaggcctgt ccaaaggtat cctttgagcc
    10801 aattcccata cattattgtg ccccggctgg ttttgcgatt ctaaaatgta ataataagac
    10861 gttcaatgga acaggaccat gtacaaatgt cagcacagta caatgtacac atggaatcag
    10921 gccagtagta tcaactcaac tgctgttaaa tggcagtcta gcagaagaag atgtagtaat
    10981 tagatctgcc aatttcacag acaatgctaa aaccataata gtacagctga acacatctgt
    11041 agaaattaat tgtacaagac ccaacaacaa tacaagaaaa agtatccgta tccagagggg
    11101 accagggaga gcatttgtta caataggaaa aataggaaat atgagacaag cacattgtaa
    11161 cattagtaga gcaaaatgga atgccacttt aaaacagata gctagcaaat taagagaaca
    11221 atttggaaat aataaaacaa taatctttaa gcaatcctca ggaggggacc cagaaattgt
    11281 aacgcacagt tttaattgtg gaggggaatt tttctactgt aattcaacac aactgtttaa
    11341 tagtacttgg tttaatagta cttggagtac tgaagggtca aataacactg aaggaagtga
    11401 cacaatcaca ctcccatgca gaataaaaca atttataaac atgtggcagg aagtaggaaa
    11461 agcaatgtat gcccctccca tcagtggaca aattagatgt tcatcaaata ttactgggct
    11521 gctattaaca agagatggtg gtaataacaa caatgggtcc gagatcttca gacctggagg
    11581 aggcgatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    11641 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    11701 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    11761 agcgtcaatg acgctgacgg tacaggccag acaattattg tctgatatag tgcagcagca
    11821 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    11881 catcaaacag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    11941 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    12001 tagttggagt aataaatctc tggaacagat ttggaataac atgacctgga tggagtggga
    12061 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    12121 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    12181 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    12241 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    12301 ttcaccatta tcgtttcaga cccacctccc aatcccgagg ggacccgaca ggcccgaagg
    12361 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    12421 cttagcactt atctgggacg atctgcggag cctgtgcctc ttcagctacc accgcttgag
    12481 agacttactc ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg ggtgggaagc
    12541 cctcaaatat tggtggaatc tcctacaata ttggagtcag gagctaaaga ataatgctgt
    12601 tagcttgctc aatgccacag ccatagcagt agctgagggg acagataggg ttatagaagt
    12661 agtacaagga gcttgtagag ctattcgcca catacctaga agaataagac agggcttgga
    12721 aaggattttg ctataagatg ggtggcgcgg ccgctgaaga cgccaaaaac ataaagaaag
    12781 gcccggcgcc attctatcct ctagaggatg gaaccgctgg agagcaactg cataaggcta
    12841 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga
    12901 acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat
    12961 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta
    13021 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata
    13081 atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca
    13141 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta
    13201 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat
    13261 ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca
    13321 aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc
    13381 ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc
    13441 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt
    13501 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag
    13561 aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa
    13621 ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac
    13681 acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac
    13741 gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc
    13801 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg
    13861 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat
    13921 tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg
    13981 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg
    14041 aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga tatcaggtgg
    14101 cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac gcgggcgtgg
    14161 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg
    14221 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga
    14281 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac
    14341 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat
    14401 tgctcgagac ctaaaaaaac atggagcaat cacaagtagc aatacagcag ctaacaatgc
    14461 tgcttgtgcc tggctaaaag cacaagagga ggaagaggtg ggttttccag tcacacctca
    14521 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
    14581 aaagggggga ctggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
    14641 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccaggggt
    14701 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
    14761 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatggaat
    14821 ggatgaccct gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
    14881 cgtggcccga gagctgcatc cggagtactt caagaactgc tgacatcgag cttgctacaa
    14941 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
    15001 agccctcaga tgctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
    15061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    15121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    15181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcacccag gaggtagagg
    15241 ttgcagtgag ccaagatcgc gccactgcat tccagcctgg gcaagaaaac aagactgtct
    15301 aaaataataa taataagtta agggtattaa atatatttat acatggaggt cataaaaata
    15361 tatatatttg ggctgggcgc agtggctcac acctgcgccc ggccctttgg gaggccgagg
    15421 caggtggatc acctgagttt gggagttcca gaccagcctg accaacatgg agaaacccct
    15481 tctctgtgta tttttagtag attttatttt atgtgtattt tattcacagg tatttctgga
    15541 aaactgaaac tgtttttcct ctactctgat accacaagaa tcatcagcac agaggaagac
    15601 ttctgtgatc aaatgtggtg ggagagggag gttttcacca gcacatgagc agtcagttct
    15661 gccgcagact cggcgggtgt ccttcggttc agttccaaca ccgcctgcct ggagagaggt
    15721 cagaccacag ggtgagggct cagtccccaa gacataaaca cccaagacat aaacacccaa
    15781 caggtccacc ccgcctgctg cccaggcaga gccgattcac caagacggga attaggatag
    15841 agaaagagta agtcacacag agccggctgt gcgggagaac ggagttctat tatgactcaa
    15901 atcagtctcc ccaagcattc ggggatcaga gtttttaagg ataacttagt gtgtaggggg
    15961 ccagtgagtt ggagatgaaa gcgtagggag tcgaaggtgt ccttttgcgc cgagtcagtt
    16021 cctgggtggg ggccacaaga tcggatgagc cagtttatca atccgggggt gccagctgat
    16081 ccatggagtg cagggtctgc aaaatatctc aagcactgat tgatcttagg ttttacaata
    16141 gtgatgttac cccaggaaca atttggggaa ggtcagaatc ttgtagcctg tagctgcatg
    16201 actcctaaac cataatttct tttttgtttt ttttttttta tttttgagac agggtctcac
    16261 tctgtcacct aggctggagt gcagtggtgc aatcacagct cactgcagcc tcaacgtcgt
    16321 aagctcaagc gatcctccca cctcagcctg cctggtagct gagactacaa gcgacgcccc
    16381 agttaatttt tgtatt
//
