LOCUS       sequence-472956-        9802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector, expresses Ascl1 under control of 
            TetON promoter and two shREST sequences under control of U6 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Nolbrant S, Giacomoni J, Hoban DB, Bruzelius A, Birtele M, 
            Chandler-Militello D, Pereira M, Ottosson DR, Goldman SA, Parmar M
  TITLE     Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial 
            Progenitor Cells into Midbrain Dopaminergic Neurons.
  JOURNAL   Stem Cell Reports. 2020 Oct 13;15(4):869-882. doi: 
            10.1016/j.stemcr.2020.08.013. Epub 2020 Sep 24.
  PUBMED    32976765
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2020.08.013"; journalName: "Stem Cell Reports"; 
            date: "2020-10-13- 13"; volume: "15"; issue: "4"; pages: "869-882"
COMMENT     Sequence Label: pLV.tetO.Ascl1.U6.shRest1.U6.shRest2
FEATURES             Location/Qualifiers
     source          1..9802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(171..189,190..212,213..231,232..254,255..273,274..296,
                     297..315,316..338,339..357,358..380,381..399,400..422,
                     423..441)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        474..512
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     509..533
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      616..625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1376..1624
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        1718..1966
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_feature    2051..2639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2104..2124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2522..2533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2642..2658)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2643..2659)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3164..3344
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3370..3387)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3376..3600
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3646..4074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3733..3752)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3943..3964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4083..4103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4088..4417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4268..4403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4330..4349
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4465..4512
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4531..4905
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5035..5156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5072..5091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5126..5145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5205..5221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5205..5221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5218..5240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5229..5245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5253..5259,5260..5277,5278..5283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5298..5319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5436..5453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5607..6195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5687..5706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6366..7157,7158..7226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6989..7008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7227..7331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7406..7425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7597..7976
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7978..8176
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8194..8374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8421..8546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9039..9272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9457..9501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9799..9802,1..114)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
       61 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      121 tattacaggg acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag
      181 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      241 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      301 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      361 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      421 actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc
      481 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      541 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg
      601 gccccgaatt cgctagccac catggagagc tctggcaaga tggagagtgg agccggccag
      661 cagccgcagc ccccgcagcc cttcctgcct cccgcagcct gcttctttgc gaccgcggcg
      721 gcggcggcag cggcggcggc cgcggcagct cagagcgcgc agcagcaaca gccgcaggcg
      781 ccgccgcagc aggcgccgca gctgagcccg gtggccgaca gccagccctc agggggcggt
      841 cacaagtcag cggccaagca ggtcaagcgc cagcgctcgt cctctccgga actgatgcgc
      901 tgcaaacgcc ggctcaactt cagcggcttc ggctacagcc tgccacagca gcagccggcc
      961 gccgtggcgc gccgcaacga gcgcgagcgc aaccgggtca agttggtcaa cctgggcttt
     1021 gccaccctcc gggagcatgt ccccaacggc gcggccaaca agaagatgag caaggtggag
     1081 acgctgcgct cggcggtcga gtacatccgc gcgctgcagc agctgctgga cgagcacgac
     1141 gcggtgagcg ctgcctttca ggcgggcgtc ctgtcgccca ccatctcccc caactactcc
     1201 aacgacttga actctatggc gggttctccg gtctcgtcct actcctccga cgagggatcc
     1261 tacgaccctc ttagcccaga ggaacaagag ctgctggact ttaccaactg gttctgagaa
     1321 ttcgatatca agcttatcga tacgagacta gcctcgagcg gccgccccct tcaccgaggg
     1381 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     1441 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     1501 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     1561 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     1621 caccggtact tcaataagga cttgctccac acgtgaggag caagtcctta ttgaagtttt
     1681 ttgaattctc gaccggatcc cggccgcccc cttcaccgag ggcctatttc ccatgattcc
     1741 ttcatatttg catatacgat acaaggctgt tagagagata attggaatta atttgactgt
     1801 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
     1861 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
     1921 tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccggta gtacacatta
     1981 accaaatggc acacgtgagc catttggtta atgtgtactt ttttgaattc tcgacctcga
     2041 gaaggtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2101 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2161 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2221 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2281 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2341 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2401 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2461 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2521 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2581 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     2641 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     2701 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     2761 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     2821 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     2881 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     2941 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3001 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     3061 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     3121 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     3181 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3241 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3301 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     3361 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3421 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3481 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3541 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3601 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3661 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3721 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3781 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3841 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3901 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3961 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4021 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4081 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4141 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4201 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4261 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4321 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4381 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4441 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4501 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     4561 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     4621 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     4681 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     4741 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     4801 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     4861 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     4921 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4981 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     5041 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5101 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5161 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7321 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7381 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7441 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7501 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7561 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     7621 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7681 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7741 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7801 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7861 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7921 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7981 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8041 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8101 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8161 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8221 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8281 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8341 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8401 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8461 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8521 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8581 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8641 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8701 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8761 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8821 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8881 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     8941 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9001 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9061 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9121 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9181 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9241 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9301 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9361 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9421 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9481 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9541 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9601 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9661 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     9721 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     9781 ggcagtattc atccacaatt tt
//
