LOCUS       sequence-472957-        9605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector, expresses Lmx1a under control of 
            TetON promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9605)
  AUTHORS   Nolbrant S, Giacomoni J, Hoban DB, Bruzelius A, Birtele M, 
            Chandler-Militello D, Pereira M, Ottosson DR, Goldman SA, Parmar M
  TITLE     Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial 
            Progenitor Cells into Midbrain Dopaminergic Neurons.
  JOURNAL   Stem Cell Reports. 2020 Oct 13;15(4):869-882. doi: 
            10.1016/j.stemcr.2020.08.013. Epub 2020 Sep 24.
  PUBMED    32976765
REFERENCE   2  (bases 1 to 9605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2020.08.013"; journalName: "Stem Cell Reports"; 
            date: "2020-10-13- 13"; volume: "15"; issue: "4"; pages: "869-882"
COMMENT     Sequence Label: pLV.tetO.lmx1a
FEATURES             Location/Qualifiers
     source          1..9605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(171..189,190..212,213..231,232..254,255..273,274..296,
                     297..315,316..338,339..357,358..380,381..399,400..422,
                     423..441)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        474..512
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     509..533
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     RBS             677..688
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    1854..2442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1907..1927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2325..2336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2445..2461)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2446..2462)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2967..3147
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3173..3190)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3179..3403
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3449..3877
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3536..3555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3746..3767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3886..3906)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3891..4220
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4071..4206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4133..4152
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4268..4315
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4334..4708
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4838..4959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4875..4894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4929..4948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5008..5024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5008..5024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5021..5043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5032..5048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5056..5062,5063..5080,5081..5086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5101..5122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5239..5256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5410..5998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5490..5509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6169..6960,6961..7029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6792..6811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7030..7134)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7209..7228)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7400..7779
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7781..7979
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7997..8177
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8224..8349
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8842..9075
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9260..9304
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9602..9605,1..114)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
       61 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      121 tattacaggg acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag
      181 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      241 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      301 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      361 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      421 actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc
      481 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      541 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg
      601 gccccgaatt cgctagcatg ttggacggcc tgaagatgga ggagaacttt caaagtgcga
      661 ttgagacctc ggcatctttc tcctctttgc tgggcagagc ggtgagcccc aagtctgtct
      721 gcgagggctg tcagcgggtc atctcggaca ggtttctgct gcggctcaac gacagcttct
      781 ggcacgagca atgcgtgcag tgtgcctcct gcaaagagcc cctggagacc acctgcttct
      841 accgggacaa gaagctctac tgcaagtacc actacgagaa actgtttgct gtcaaatgtg
      901 ggggctgctt cgaggccatt gcgcccaatg agtttgtcat gcgtgcccag aagagcgtat
      961 accacctgag ctgcttctgc tgctgcgtct gtgagcgaca gctgcagaag ggtgacgagt
     1021 ttgtcctgaa ggagggccag ctgctctgca aaggggacta tgagaaagaa cgggagctgc
     1081 tgagcctggt gagccctgcg gcctcagact caggcaaaag cgatgatgag gagagccttt
     1141 gcaagtcagc ccatggggca ggaaaaggag catcagagga cggcaaggac cataagcgac
     1201 ccaaacgtcc cagaaccatc ctgaccactc agcagaggag agcattcaag gcctcgtttg
     1261 aagtatcctc caagccctgc agaaaggtga gggagactct ggctgcagag acagggctga
     1321 gtgtccgtgt ggttcaggta tggttccaga accagcgagc caagatgaag aagctggccc
     1381 ggcgacagca gcaacagcaa caggaccaac agaacaccca gaggctgact tctgctcaga
     1441 caaatggtag tgggaatgcg ggcatggaag ggatcatgaa cccctataca acattgccca
     1501 ccccacagca gctgctggcc attgaacaga gcgtctacaa ctctgatccc ttccgacagg
     1561 gtctcacccc accccagatg cctggagatc acatgcaccc ctatggtgct gaacctcttt
     1621 tccatgactt ggatagtgat gacacatctc tcagtaacct gggagactgc ttcctggcaa
     1681 cctcagaagc tgggcctctg cagtccagag tgggaaaccc cattgaccat ctgtactcca
     1741 tgcagaattc ctatttcacc tcttgaggat cctacgaccc tcttagccca gaggaacaag
     1801 agctgctgga ctttaccaac tggttctgag aattcgatat caagcttatc gataatcaac
     1861 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     1921 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     1981 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2041 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2101 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2161 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2221 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2281 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2341 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2401 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     2461 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     2521 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     2581 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     2641 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     2701 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     2761 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     2821 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     2881 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     2941 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3001 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3061 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3121 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     3181 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3241 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3301 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3361 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     3421 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     3481 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3541 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3601 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3661 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3721 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3781 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3841 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     3901 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3961 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4021 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4141 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4201 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4261 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     4321 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     4381 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     4441 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     4501 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     4561 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     4621 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     4681 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     4741 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4801 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     4861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     4981 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5041 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7141 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     7201 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     7261 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     7321 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     7381 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     7441 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7501 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7561 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7621 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     7681 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     7741 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     7801 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     7861 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     7921 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
     7981 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8161 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     8221 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     8281 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     8341 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     8401 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     8461 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8521 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8581 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8641 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8701 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8761 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8821 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8881 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8941 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9001 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9061 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9121 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9181 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9241 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9301 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9361 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9421 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     9481 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     9541 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     9601 atttt
//
