LOCUS       sequence-472958-       10251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector, expresses Nurr1 under control of 
            TetON promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Nolbrant S, Giacomoni J, Hoban DB, Bruzelius A, Birtele M, 
            Chandler-Militello D, Pereira M, Ottosson DR, Goldman SA, Parmar M
  TITLE     Direct Reprogramming of Human Fetal- and Stem Cell-Derived Glial 
            Progenitor Cells into Midbrain Dopaminergic Neurons.
  JOURNAL   Stem Cell Reports. 2020 Oct 13;15(4):869-882. doi: 
            10.1016/j.stemcr.2020.08.013. Epub 2020 Sep 24.
  PUBMED    32976765
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2020.08.013"; journalName: "Stem Cell Reports"; 
            date: "2020-10-13- 13"; volume: "15"; issue: "4"; pages: "869-882"
COMMENT     Sequence Label: pLV.tetO.Nurr1
FEATURES             Location/Qualifiers
     source          1..10251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(171..189,190..212,213..231,232..254,255..273,274..296,
                     297..315,316..338,339..357,358..380,381..399,400..422,
                     423..441)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        474..512
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     509..533
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2500..3088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2553..2573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2971..2982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3091..3107)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3092..3108)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3613..3793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3819..3836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3825..4049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4095..4523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4182..4201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4392..4413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4532..4552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4537..4866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4717..4852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4779..4798
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4914..4961
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4980..5354
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5484..5605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5521..5540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5575..5594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5654..5670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5654..5670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5667..5689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5678..5694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5702..5708,5709..5726,5727..5732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5747..5768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5885..5902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6056..6644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6136..6155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6815..7606,7607..7675))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7438..7457
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7676..7780)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7855..7874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8046..8425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8427..8625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8643..8823
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8870..8995
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9488..9721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9906..9950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10248..10251,1..114)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
       61 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      121 tattacaggg acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag
      181 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      241 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      301 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      361 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      421 actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc
      481 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      541 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg
      601 gccccgaatt cgctagatgc cttgtgttca ggcgcagtat gggtcctcgc ctcaaggagc
      661 cagccccgct tctcagagct acagttacca ctcttcggga gaatacagct ccgatttctt
      721 aactccagag tttgtcaagt ttagcatgga cctcaccaac actgaaatta ctgccaccac
      781 ttctctcccc agcttcagta cctttatgga caactacagc acaggctacg acgtcaagcc
      841 accttgcttg taccaaatgc ccctgtccgg acagcagtcc tccattaagg tagaagacat
      901 tcagatgcac aactaccagc aacacagcca cctgccccct cagtccgagg agatgatgcc
      961 acacagcggg tcggtttact acaagccctc ttcgcccccg acacccagca ccccgagctt
     1021 ccaggtgcag catagcccga tgtgggacga tccgggctcc cttcacaact tccaccagaa
     1081 ctacgtggcc actacgcata tgatcgagca gaggaagaca cctgtctccc gcctgtcact
     1141 cttctccttt aagcagtcgc ccccgggcac tcctgtgtct agctgccaga tgcgcttcga
     1201 cgggcctctg cacgtcccca tgaacccgga gcccgcgggc agccaccacg tagtggatgg
     1261 gcagaccttc gccgtgccca accccattcg caagccggca tccatgggct tcccgggcct
     1321 gcagatcggc cacgcatcgc agttgcttga cacgcaggtg ccctcgccgc cgtcccgggg
     1381 ctctccctcc aatgagggtc tgtgcgctgt ttgcggtgac aacgcggcct gtcagcacta
     1441 cggtgttcgc acttgtgagg gctgcaaagg tttctttaag cgcacggtgc aaaaaaacgc
     1501 gaaatatgtg tgtttagcaa ataaaaactg cccagtggac aagcgccgcc gaaatcgttg
     1561 tcagtactgt cggtttcaga agtgcctagc tgttgggatg gttaaagaag tggttcgcac
     1621 ggacagttta aaaggccgga gaggtcgttt accctcgaag ccgaagagcc cacaggatcc
     1681 ctctcccccc tcacctccgg tgagtctgat cagtgccctc gtcagagccc acgtcgattc
     1741 caatccggca atgaccagcc tggactattc caggttccag gcaaaccctg actatcagat
     1801 gagtggagat gatacccaac atatccagca gttctacgat ctcctgaccg gctctatgga
     1861 gatcatcaga gggtgggcag agaagatccc tggctttgct gacctgccca aagccgacca
     1921 ggacctgctt tttgaatcag ctttcttaga attatttgtt ctgcgcttag catacaggtc
     1981 caacccagtg gagggtaaac tcatcttttg caatggggtg gtcttgcaca ggttgcaatg
     2041 cgtgcgtggc tttggggaat ggattgattc cattgttgaa ttctcctcca acttgcagaa
     2101 tatgaacatc gacatttctg ccttctcctg cattgctgcc ctggctatgg tcacagagag
     2161 acacgggctc aaggaaccca agagagtgga agagctacaa aacaaaattg taaattgtct
     2221 taaagaccat gtgactttca ataatggggg tttgaaccga cccaactacc tgtctaaact
     2281 gttggggaag ctgccagaac tccgcaccct ttgcacacag ggcctccagc gcattttcta
     2341 cctgaaattg gaagacttgg taccaccacc agcaataatt gacaaacttt tcctggacac
     2401 cttacctttc taagatccta cgaccctctt agcccagagg aacaagagct gctggacttt
     2461 accaactggt tctgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     2521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2881 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     2941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3061 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     3121 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     3181 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     3241 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     3301 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     3361 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     3421 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     3481 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     3541 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     3601 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3661 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3721 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3781 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4081 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4501 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4621 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4801 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4861 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     4921 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     4981 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     5041 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     5101 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     5161 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     5221 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     5281 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     5341 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     5401 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5461 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5521 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5581 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     5641 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5821 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7801 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     7861 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     7921 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     7981 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     8041 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     8101 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     8161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     8221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     8281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     8341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     8401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     8461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     8521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     8581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     8641 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8701 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8761 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8821 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8881 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8941 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     9001 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9061 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9121 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9181 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9241 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9301 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     9361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    10021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    10081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
    10141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
    10201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt t
//
