LOCUS       sequence-47296-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-Delta 12a
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    174..198
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(654..678)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    731..852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(742..761)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     796..815
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 accgacgacg tgagcgtcgc gctgagaaac aagctcaatg gaaagctgca aacccaccgc
       61 tcgaggctgc ccaagcttac accatggcct acccatatga tgttccagat tacgcttcac
      121 tcgacgcgga tcccgtcgaa ttcacgcgtg gtacctctag agtcgaccca tcaacaagtt
      181 tgtacaaaaa agcaggcttc ttcacttggg gaaacaacat aaatccaaat aattcatcag
      241 gatgggatga atcttctaaa cctactcctt cccagggatg gggagaccct ccaaagtcta
      301 atcagtctct aggttgggga gattcgtcaa agccagtcag ctctccagac tggaacaagc
      361 aacaagacat tgttggatct tggggaatcc caccagctac aggcaaacct cctggtacag
      421 gctggctggg gggacctata ccagccccag caaaagaaga agaacccaca ggctgggagg
      481 aaccatcccc agaatctata cgtcgcaaaa tggagattga tgatggaact tcagcttggg
      541 gagatccaag caaatacaac tacaaaaatg tgaacatgtg gaacaaaaac gtcccaaatg
      601 gcaacagccg ttcagaccag caagcacagg tacatcagct gctaacgcct tgaacccagc
      661 tttcttgtac aaagtggttg atgggcggcc gcttcccttt agtgagggtt aatgcttcga
      721 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      781 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      841 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca ggg
//
