LOCUS       sequence-47299-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-Ago2
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..130
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             167..193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             173..199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gtcctccgtt cattcagctc ttaaggctag agtacttaat acgactcact ataggctagc
       61 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      121 agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc catatgatgt
      181 tccagattac gcttcactcg acgcggatcc cgtcgaattc accatgggag ggcacccatt
      241 ccagtggtgt aacgcacttg cacctcctgc gccgccgccc cccatccaag gatatgcctt
      301 caagcctcca cctagacccg actttgggac ctccgggaga acaatcaaat tacaggccaa
      361 tttcttcgaa atggacatcc ccaaaattga catctatcat tatgaattgg atatcaagcc
      421 agagaagtgc ccgaggagag ttaacaggga aatcgtggaa cacatggtcc agcactttaa
      481 aacacagatc tttggggatc ggaagcccgt gtttgacggc aggaagaatc tatacacagc
      541 catgcccctt ccgattggga gggacaaggt ggagctggag gtcacgctgc caggagaagg
      601 caaggatcgc atcttcaagg tgtccatcaa gtgggtgtcc tgcgtgagct tgcaggcgtt
      661 acacgatgca ctttcagggc ggctgcccag cgtccctttt gagacgatcc aggccctgga
      721 cgtggtcatg aggcacttgc catccatgag gtacaccccc gtgggccgct ccttcttcac
      781 cgcgtccgaa ggctgctcta accctcttgg cgggggccga gaagtgtggt ttggcttcca
      841 tcagtccgtc cggccttctc tctggaaaat gatgctgaat
//
