LOCUS       sequence-473010-         195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown expression of Daglb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 195)
  AUTHORS   Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, Guo J, Tan J, Dong A, 
            He K, Zhou J, Cinar R, Wu J, Salinas AG, Sun L, Kumar M, Sullivan 
            BT, Oldham BB, Pitz V, Makarious MB, Ding J, Kung J, Xie C, Hawes 
            SL, Wang L, Wang T, Chan P, Zhang Z, Le W, Chen S, Lovinger DM, 
            Blauwendraat C, Singleton AB, Cui G, Li Y, Cai H, Tang B
  TITLE     Deficiency in endocannabinoid synthase DAGLB contributes to early 
            onset Parkinsonism and murine nigral dopaminergic neuron 
            dysfunction.
  JOURNAL   Nat Commun. 2022 Jun 17;13(1):3490. doi: 10.1038/s41467-022-31168-9.
  PUBMED    35715418
REFERENCE   2  (bases 1 to 195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-17"; volume: "13(1):3490. doi"; pages: " 
            10.1038/s41467-022-31168-9"
COMMENT     Sequence Label: pAAV-CMV-DIO-SaCas9-U6-sgDaglb
FEATURES             Location/Qualifiers
     source          1..195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        56..131
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggctttatat atcttgtgga aggacgaaac accgcccatc attgaccagt ctgcggtttt
       61 agtactctgg aaacagaatc tactaaaaca aggcaaaatg ccgtgtttat ctcgtcaact
      121 tgttggcgag atttttcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
      181 tccctctctg cgcgc
//
