LOCUS       sequence-473021-         362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown expression of Gabbr1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Dong J, Wang L, Sullivan BT, Sun L, Martinez Smith VM, Chang L, Ding
            J, Le W, Gerfen CR, Cai H
  TITLE     Molecularly distinct striatonigral neuron subtypes differentially 
            regulate locomotion.
  JOURNAL   Nat Commun. 2025 Mar 19;16(1):2710. doi: 10.1038/s41467-025-58007-x.
  PUBMED    40108161
REFERENCE   2  (bases 1 to 362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-03-19"; volume: "16(1):2710. doi"; pages: " 
            10.1038/s41467-025-58007-x"
COMMENT     Sequence Label: pAAV-CMV-DIO-SaCas9-U6-sgGabbr1
FEATURES             Location/Qualifiers
     source          1..362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        225..300
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgcgccaca ctccacaatc ccacgtttta gtactctgga
      241 aacagaatct actaaaacaa ggcaaaatgc cgtgtttatc tcgtcaactt gttggcgaga
      301 tttttcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
      361 gc
//
