LOCUS       sequence-47303-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-Delta 12b
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(593..617)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(669..695)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(675..701)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      800..809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(813..831)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcattaccc tcactaaagg gaagcggccg cccatcaacc actttgtaca agaaagctgg
       61 gttcactctg gtgagtctct cgaaaaactg atgtttgaaa actgtttaac aaatggattt
      121 atccacgctt cttcttgttt gtttccacct ttcatcattc ccctttccct tctccttttt
      181 ttgccactca gacccttcga tgacattttc tttgtatatg gtggccaatt taaagatgag
      241 ttaagctctt gagatgaatt actattccac attccaatct ccacatcctc ttcctcttcc
      301 cagccagtgg ggcgatttcc aggcaatggc atatcatcac cacaccagcc atcttgcata
      361 gattttggcc cagatttgct taatgcttgt ggaccaacag agctggagcc ccacgtggat
      421 gtgctggatg ccgcagcaat gggttccccc cagctgggac cactgtctat gggcttaccc
      481 catgctgaag taccattatc cacagttgtg gctggagtag aaggctcccc ccagggctca
      541 ccccagccag agccactgct tgcctctttg tttgagatgg cacttgcagg gaagcctgct
      601 tttttgtaca aacttgttga tgggtcgact ctagaggtac cacgcgtgaa ttcgacggga
      661 tccgcgtcga gtgaagcgta atctggaaca tcatatgggt aggccatggt gtaagcttgg
      721 gcagcctcga gcggtgggtt tgcagctttc cattgagctt gtttctcagc gcgacgctca
      781 cgtcgtcgtg nttgtgcgtc catggtggct agcctatagt gagtcgtatt aagtactcta
      841 gccttaagag ctgtaattga actgggagtg gacacctgtg gagagaaggc aaagtggatg
      901 tcagtaacaa
//
