LOCUS       sequence-47304-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan 
            EK
  TITLE     Divergent GW182 functional domains in the regulation of 
            translational silencing.
  JOURNAL   Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
  PUBMED    21131274
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-04"; volume: "39(7)"; pages: "2534-47. Epub
            2010 Dec 2"
COMMENT     Sequence Label: NHA-Delta 12am
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(518..542)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(594..620)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(600..626)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(663..728)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      725..734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(738..756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(799..818)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 agcattaccc tcactaaagg gaagcggccg cccatcaacc actttgtaca agaaagctgg
       61 gttcaaggcg ttagcagctg atgtacctgt gcttgctggt ctgaacggct gttgccattt
      121 gggacgtttt tgttccacat gttcacattt ttgtagttgt atttgcttgg atcagcagca
      181 gctgaagttc catcatcaat ctccattttg cgacgtatag attctgggga tggttcctca
      241 gcagctgtgg gttcttcttc ttttgctggg gctggtatag gtccccccag agcagctgta
      301 ccaggaggtt tgcctgtagc tggtgggata gcagcagatc caacaatgtc ttgttgcttg
      361 ttccagtctg gagagctgac tggctttgac gaatctccag cagctagaga ctgattagac
      421 tttggagggt ctccagcagc ctgggaagga gtaggtttag aagattcatc agcagctgat
      481 gaattatttg gatttatgtt gttagcagca gtgaagaagc ctgctttttt gtacaaactt
      541 gttgatgggt cgactctaga ggtaccacgc gtgaattcga cgggatccgc gtcgagtgaa
      601 gcgtaatctg gaacatcata tgggtaggcc atggtgtaag cttgggcagc ctcgagcggt
      661 gggtttgcag ctttccattg agcttgtttc tcagcgcgac gctcacgtcg tcgtgtttgt
      721 gcgtccatgg tggctagcct atagtgagtc gtattaagta ctctagcctt aagagctgta
      781 attgaactgg gagtggacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      841 aggtgcctat cagaaacgca agagtcttct ctgtctcgac angcccagtt ccaatgggct
      901 cctacgtgac
//
