LOCUS       sequence-473045-       10933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1692
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3871..4459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3924..3944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4342..4353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4531..4764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4842..4963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4879..4898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4933..4952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5003..5138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5158..5177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5159..5177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5187..5204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5187..5203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5196..5218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5345..5800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5432..5451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5642..5663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5826..5930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5931..5999,6000..6791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6149..6168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6962..7550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7451..7470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7704..7721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7838..7859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7874..7879,7880..7897,7898..7904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7912..7928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7917..7939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7936..7952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7936..7952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7971..7991
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7973..7991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8019..8245
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8246..8426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8473..8598
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9091..9324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9509..9553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9784..9994
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9806..10002
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9853..9988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10034..10636
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10034..10053)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10530..10550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10679..10796
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 tgccgcggct acgctgaggc tctccgctca gggcacagtg acttttgaag atgtggctgt
     1261 gaactttacc tgggaggaat ggaatctcct tagtgaggct cagagatgcc tgtaccgtga
     1321 tgtgacgctg gagaacctgg cacttatatc ctccctgggt tgttggtgtg gagtggaaga
     1381 tgaggcggca ccttctaagc agagtattta tatacaaaga gagactcagg tcaggactcc
     1441 tatggcaggt gtgtctccca agaaggccca cccctgtgag atgtgtggcc cgatcttggg
     1501 agacattttg catgtggcag atcatcaggg aacacatcac aagcagaaac tgcacaggtg
     1561 tgaggcctgg gggaataaat tgtatgacag tggaaacttt catcagcacc agaatgagca
     1621 cattggagag aaaccctaca gagggagtgt tgaggaggcg ttgtttgcaa agaggtgtaa
     1681 gttgcatgtg tcaggggagt catctgtctt cagtgagagt gggaaggact ttttgcccag
     1741 gtcaggatta ctccagcagg aggccagtca cactggggag aagtcaaaca gcaaaactga
     1801 gtgtgtgtct cccattcagt gtgggggagc tcactacagc tgtggagaat ccatgaaaca
     1861 ttttagcacc aaacatatac tcagtcagca ccagagactg ctcactagag aagagtgtta
     1921 tgtgtgctgt gaatgtggga agtcctttag caaatatgct agcttgagta atcatcagag
     1981 agttcacact gaaaaaaaac atgaatgtgg agaatgtggg aaatccttta gcaaatatgt
     2041 tagcttcagt aatcatcaga gagttcacac tgaaaaaaaa catgaatgtg gagaatgtgg
     2101 gaaatccttt agcaaatatg ttagcttcag taatcatcag agagttcaca ctgggaaaag
     2161 accttatgaa tgtggagaat gtgggaaatc gtttagcaaa tatgctagct tcagtaatca
     2221 tcagagagtt cacactgaaa aaaaacatta tgaatgtgga gaatgtggga aatcctttag
     2281 caaatatgtt agcttcagta atcatcagag agttcacact gggaaaagac cttatgaatg
     2341 tggagaatgt gggaaatcgt ttagcaaata tgctagcttc agtaatcatc agagagttca
     2401 cactgacaaa aaacattatg aatgtggaga atgtgggaaa tcctttagtc aaaagagcag
     2461 cctcattcaa catcagcgat ttcacactgg agaaaaacct tatgggtgtg aagaatgtgg
     2521 gaaatctttt agttcagaag gacatcttag gagccatcaa cgagttcacg ccggagaaag
     2581 acctttcaag tgtggagaat gtgtgaaatc tttcagtcat aagcgcagcc ttgttcacca
     2641 tcagcgagtt cacagtggag aaagacctta tcagtgtgga gaatgtggga aatctttcag
     2701 tcaaaagggc aacctcgttc tacaccagcg agttcacact ggagcaagac cttatgagtg
     2761 tggagaatgt gggaaatcat ttagttcaaa aggacatctt aggaaccatc agcaaattca
     2821 cactggggac agactttatg agtgtggaga gtgtgggaaa tcttttagtc ataaaggcac
     2881 cctcattcta catcagcgag ttcaccctag agaaagatct tatgggtgtg gagaatgtgg
     2941 gaaatctttt agttcaatcg ggcaccttag gagccatcag cgcgttcata ctggagagag
     3001 gccttatgag tgtggagaat gtgggaaatc ttttagtcat aagcgcagcc ttgttcacca
     3061 tcagcgcatg cacactggag aaagacctta caagtgtgga gactgtggga aatcttttaa
     3121 tgaaaaagga caccttagga atcatcagcg agttcacact acagaaagac cttttaagtg
     3181 tggggaatgt gggaaatgtt ttagtcacaa gggtaacctc attctacacc agcatggcca
     3241 tactggagaa agaccttatg tatgtaggga atgtggaaaa ttatttaaga agaagtctca
     3301 cctccttgta caccagagaa ttcacaatgg agaaaagcca tatgcttgtg aagcttgtca
     3361 gaaatttttt agaaacaagt accaactcat tgcacatcag agagttcaca ctggagaaag
     3421 gccttatgaa tgcaatgatt gtggaaaatc atttacccac agctctacat tctgtgttca
     3481 taagcgaatt cacactggag aaaagcctta tgagtgcagt gaatgtggaa aatctttcgc
     3541 tgaaagctcc agtttcacaa aacacaaaag agttcacact ggagaaaagc cttatgagtg
     3601 cagtgaatgt ggaaaatctt ttgctgaaag ctccagtctc actaaacaca agagagttca
     3661 cactggagaa aagccttata aatgtgagaa atgtgggaaa ttatttaaca agaagtctca
     3721 cctccttgta caccagagtt cacactggag aaaagccata tagtaatgaa ctagtccgta
     3781 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg agtccacgag
     3841 aactttggat acgaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4021 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4081 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4141 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4201 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4261 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4321 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4381 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4441 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     4501 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4561 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4741 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     4801 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     4861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4981 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5101 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     5161 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     5221 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5281 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5341 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5581 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5641 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5701 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5761 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5881 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5941 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6001 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6061 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6121 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6181 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6241 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6301 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6361 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6421 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6481 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6541 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6601 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6661 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6721 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6781 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6841 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6901 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6961 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7021 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7081 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7141 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7201 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7261 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7321 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7381 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7441 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7501 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7561 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7621 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7681 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7741 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7801 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7861 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7921 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7981 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     8041 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     8101 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     8161 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     8221 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     8281 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8341 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8401 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     8461 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     8521 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8581 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8641 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8701 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8761 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8821 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8881 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8941 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     9001 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     9061 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     9121 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     9181 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     9241 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     9301 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     9361 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     9421 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     9481 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     9541 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     9601 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     9661 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     9721 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9781 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     9841 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9901 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9961 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
    10021 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
    10081 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
    10141 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
    10201 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
    10261 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
    10321 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
    10381 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    10441 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    10501 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    10561 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    10621 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
    10681 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    10741 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    10801 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
    10861 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    10921 aggagtggga att
//
