LOCUS       sequence-47306-e         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of hChR2(H134R) for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Kozorovitskiy Y, Saunders A, Johnson CA, Lowell BB, Sabatini BL
  TITLE     Recurrent network activity drives striatal synaptogenesis.
  JOURNAL   Nature. 2012 May 13;485(7400):646-50. doi: 10.1038/nature11052.
  PUBMED    22660328
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-13- 13"; volume: "485"; issue: "7400"; 
            pages: "646-50"
COMMENT     Sequence Label: pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
       61 acttcgtata gcatacatta tacgaagtta tggcgcgcct cgccaccatg gactatggcg
      121 gcgctttgtc tgccgtcgga cgcgaacttt tgttcgttac taatcctgtg gtggtgaacg
      181 ggtccgtcct ggtccctgag gatcaatgtt actgtgccgg atggattgaa tctcgcggca
      241 cgaacggcgc tcagaccgcg tcaaatgtcc tgcagtggct tgcagcagga ttcagcattt
      301 tgctgctgat gttctatgcc taccaaacct ggaaatctac atgcggctgg gaggagatct
      361 atgtgtgcgc cattgaaatg gttaaggtga ttctcgagtt cttttttgag tttaagaatc
      421 cctctatgct ctaccttgcc acaggacacc gggtgcagtg gctgcgctat gcagagtggc
      481 tgctcacttg tcctgtcatc cttatccgcc tgagcaacct caccggcctg agcaacgact
      541 aca
//
