LOCUS       sequence-47307-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of hChR2(H134R) for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Kozorovitskiy Y, Saunders A, Johnson CA, Lowell BB, Sabatini BL
  TITLE     Recurrent network activity drives striatal synaptogenesis.
  JOURNAL   Nature. 2012 May 13;485(7400):646-50. doi: 10.1038/nature11052.
  PUBMED    22660328
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-13- 13"; volume: "485"; issue: "7400"; 
            pages: "646-50"
COMMENT     Sequence Label: pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..78)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(129..162)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(455..474)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     724..742
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attcataact tcgtatagca
       61 tacattatac gaagttattc tttgcaccat tctaaagaat aacagtgata atttctgggt
      121 taaggcaaat aacttcgtat aggatacttt atacgaagtt atgctagcgc ggccgcttac
      181 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc
      241 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg
      301 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg
      361 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg
      421 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
      481 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
      541 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
      601 aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag
      661 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca
      721 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
      781 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
      841 aactccttga tgatggccat
//
