LOCUS       sequence-473096-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-Spike
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(919..946)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1038..1124)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1173..1195
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1187..1204
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1188..1204
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1220..1319
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4910..4921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5435..5534)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5551..5570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5552..5570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5575..5591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5575..5591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6022..6813
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga
      901 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc
      961 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc
     1021 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1081 aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     1141 tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc
     1201 cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt
     1261 gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcagaagg
     1321 gccgtcaagg ccaggatcca tgggatccat acactcagtg tttctactga tgttcttgtt
     1381 aacacctaca gaaagttacg ttgatgtagg gccagattct gttaagtctg cttgtattga
     1441 ggttgatata caacagactt tctttgataa aacttggcct aggccaattg atgtttctaa
     1501 ggctgacggt attatatacc ctcaaggccg tacatattct aacataacta tcacttatca
     1561 aggtcttttt ccctatcagg gagaccatgg tgatatgtat gtttactctg caggacatgc
     1621 tacaggcaca actccacaaa agttgtttgt agctaactat tctcaggacg tcaaacagtt
     1681 tgctaatggg tttgtcgtcc gtataggagc agctgccaat tccactggca ctgttattat
     1741 tagcccatct accagcgcta ctatacgaaa aatttaccct gcttttatgc tgggttcttc
     1801 agttggtaat ttctcagatg gtaaaatggg ccgcttcttc aatcatactc tagttctttt
     1861 gcccgatgga tgtggcactt tacttagagc tttttattgt attctagagc ctcgctctgg
     1921 aaatcattgt cctgctggca attcctatac ttcttttgcc acttatcaca ctcctgcaac
     1981 agattgttct gatggcaatt acaatcgtaa tgccagtctg aactctttta aggagtattt
     2041 taatttacgt aactgcacct ttatgtacac ttataacatt accgaagatg agattttaga
     2101 gtggtttggc attacacaaa ctgctcaagg tgttcacctc ttctcatctc ggtatgttga
     2161 tttgtacggc ggcaatatgt ttcaatttgc caccttgcct gtttatgata ctattaagta
     2221 ttattctatc attcctcaca gtattcgttc tatccaaagt gatagaaaag cttgggctgc
     2281 cttctacgta tataaacttc aaccgttaac tttcctgttg gatttttctg ttgatggtta
     2341 tatacgcaga gctatagact gtggttttaa tgatttgtca caactccact gctcatatga
     2401 atccttcgat gttgaatctg gagtttattc agtttcgtct ttcgaagcaa aaccttctgg
     2461 ctcagttgtg gaacaggctg aaggtgttga atgtgatttt tcacctcttc tgtctggcac
     2521 acctcctcag gtttataatt tcaagcgttt ggtttttacc aattgcaatt ataatcttac
     2581 caaattgctt tcactttttt ctgtgaatga ttttacttgt agtcaaatat ctccagcagc
     2641 aattgctagc aactgttatt cttcactgat tttggattac ttttcatacc cacttagtat
     2701 gaaatccgat ctcagtgtta gttctgctgg tccaatatcc cagtttaatt ataaacagtc
     2761 cttttctaat cccacatgtt tgattttagc gactgttcct cataacctta ctactattac
     2821 taagcctctt aagtacagct atattaacaa gtgctctcgt cttctttctg atgatcgtac
     2881 tgaagtacct cagttagtga acgctaatca atactcaccc tgtgtatcca ttgtcccatc
     2941 cactgtgtgg gaagacggtg attattatag gaaacaacta tctccacttg aaggtggtgg
     3001 ctggcttgtt gctagtggct caactgttgc catgactgag caattacaga tgggctttgg
     3061 tattacagtt caatatggta cagacaccaa tagtgtttgc cccaagcttg aatttgctaa
     3121 tgacacaaaa attgcctctc aattaggcaa ttgcgtggaa tattccctct atggtgtttc
     3181 gggccgtggt gtttttcaga attgcacagc tgtaggtgtt cgacagcagc gctttgttta
     3241 tgatgcgtac cagaatttag ttggctatta ttctgatgat ggcaactact actgtttgcg
     3301 tgcttgtgtt agtgttcctg tttctgtcat ctatgataaa gaaactaaaa cccacgctac
     3361 tctatttggt agtgttgcat gtgaacacat ttcttctacc atgtctcaat actcccgttc
     3421 tacgcgatca atgcttaaac ggcgagattc tacatatggc ccccttcaga cacctgttgg
     3481 ttgtgtccta ggacttgtta attcctcttt gttcgtagag gactgcaagt tgcctcttgg
     3541 tcaatctctc tgtgctcttc ctgacacacc tagtactctc acacctcgca gtgtgcgctc
     3601 tgttccaggt gaaatgcgct tggcatccat tgcttttaat catcctattc aggttgatca
     3661 acttaatagt agttatttta aattaagtat acccactaat ttttcctttg gtgtgactca
     3721 ggagtacatt cagacaacca ttcagaaagt tactgttgat tgtaaacagt acgtttgcaa
     3781 tggtttccag aagtgtgagc aattactgcg cgagtatggc cagttttgtt ccaaaataaa
     3841 ccaggctctc catggtgcca atttacgcca ggatgattct gtacgtaatt tgtttgcgag
     3901 cgtgaaaagc tctcaatcat ctcctatcat accaggtttt ggaggtgact ttaatttgac
     3961 acttctagaa cctgtttcta tatctactgg cagtcgtagt gcacgtagtg ctattgagga
     4021 tttgctattt gacaaagtca ctatagctga tcctggttat atgcaaggtt acgatgattg
     4081 catgcagcaa ggtccagcat cagctcgtga tcttatttgt gctcaatatg tggctggtta
     4141 caaagtatta cctcctctta tggatgttaa tatggaagcc gcgtatactt catctttgct
     4201 tggcagcata gcaggtgttg gctggactgc tggcttatcc tcctttgctg ctattccatt
     4261 tgcacagagt atcttttata ggttaaacgg tgttggcatt actcaacagg ttctttcaga
     4321 gaaccaaaag cttattgcca ataagtttaa tcaggctctg ggagctatgc aaacaggctt
     4381 cactacaact aatgaagctt ttcagaaggt tcaggatgct gtgaacaaca atgcacaggc
     4441 tctatccaaa ttagctagcg agctatctaa tacttttggt gctatttccg cctctattgg
     4501 agacatcata caacgtcttg atgttctcga acaggacgcc caaatagaca gacttattaa
     4561 tggccgtttg acaacactaa atgcttttgt tgcacagcag cttgttcgtt ccgaatcagc
     4621 tgctctttcc gctcaattgg ctaaagataa agtcaatgag tgtgtcaagg cacaatccaa
     4681 gcgttctgga ttttgcggtc aaggcacaca tatagtgtcc tttgttgtaa atgcccctaa
     4741 tggcctttac ttcatgcatg ttggttatta ccctagcaac cacattgagg ttgtttctgc
     4801 ttatggtctt tgcgatgcag ctaaccctac taattgtata gcccctgtta atggctactt
     4861 tattaaaact aataacacta ggattgttga tgagtggtca tatactggct cgtccttcta
     4921 tgcacctgag cccattacct cccttaatac taagtatgtt gcaccacagg tgacatacca
     4981 aaacatttct actaacctcc ctcctcctct tctcggcaat tccaccggga ttgacttcca
     5041 agatgagttg gatgagtttt tcaaaaatgt tagcaccagt atacctaatt ttggttccct
     5101 aacacagatt aatactacat tactcgatct tacctacgag atgttgtctc ttcaacaagt
     5161 tgttaaagcc cttaatgagt cttacataga ccttaaagag cttggcaatt atacttatta
     5221 caacaaatgg ccgtggtaca tttggcttgg tttcattgct gggcttgttg ccttagctct
     5281 atgcgtcttc ttcatactgt gctgcactgg ttgtggcaca aactgtatgg gaaaacttaa
     5341 gtgtaatcgt tgttgtgata gatacgagga atacgacctc gagccgcata aggttcatgt
     5401 tcaccttaat taagacgcga tcgcggcctc atgggcccag ctttcttgta caaagttggc
     5461 attataaaaa ataattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     5521 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     5581 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     5641 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
     5701 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     5761 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     5821 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     5881 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     5941 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     6001 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     6061 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     6121 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     6181 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     6241 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     6301 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     6361 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     6421 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     6481 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     6541 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     6601 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     6661 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     6721 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     6781 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     6841 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6901 tcttc
//
