LOCUS       sequence-473107-        5817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing a truncated, constitutively active human insulin receptor
            (IR-beta) in astrocyte.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Frazier HN, Anderson KL, Maimaiti S, Ghoweri AO, Kraner SD, Popa GJ,
            Hampton KK, Mendenhall MD, Norris CM, Craven RJ, Thibault O
  TITLE     Expression of a Constitutively Active Human Insulin Receptor in 
            Hippocampal Neurons Does Not Alter VGCC Currents.
  JOURNAL   Neurochem Res. 2019 Jan;44(1):269-280. doi: 
            10.1007/s11064-018-2510-2. Epub 2018 Mar 23.
  PUBMED    29572644
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s11064-018-2510-2"; journalName: "Neurochem Res"; date: 
            "2019-01"; volume: "44"; issue: "1"; pages: "269-280"
COMMENT     Sequence Label: Gfa104-hIR-beta
FEATURES             Location/Qualifiers
     source          1..5817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          926..1058
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1041..1060
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1103..1121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2542..2568
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2595..2716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2606..2625)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2660..2679
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2878..3007
                     /label=AAV2 ITR
     primer_bind     complement(3026..3043)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3026..3042)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3035..3057)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3184..3639
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3271..3290)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3481..3502
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3665..3769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3770..3838,3839..4630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4801..5389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5290..5309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5677..5698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5713..5718,5719..5736,5737..5743)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5751..5767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5756..5778
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5775..5791
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5775..5791
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccttaattag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt
      121 gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta ggaagatcta
      181 acatatcctg gtgtggagta ggggacgctg ctctgacaga ggctcggggg cctgagctgg
      241 ctctgtgagc tggggaggag gcagacagcc aggccttgtc tgcaagcaga cctggcagca
      301 ttgggctggc cgccccccag ggcctcctct tcatgcccag tgaatgactc accttggcac
      361 agacacaatg ttcggggtgg gcacagtgcc tgcttcccgc cgcaccccag cccccctcaa
      421 atgccttccg agaagcccat tgagcagggg gcttgcattg caccccagcc tgacagcctg
      481 gcatcttggg ataaaagcag cacagccccc taggggctgc ccttgctgtg tggcgccacc
      541 ggcggtggag aacaaggctc tattcagcct gtgcccagga aaggggatca ggggatgccc
      601 aggcatggac agtgggtggc agggggggag aggagggctg tctgcttccc agaagtccaa
      661 ggacacaaat gggtgagggg agagctctcc ccatagctgg gctgcggccc aaccccaccc
      721 cctcaggcta tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact
      781 ccttcataaa gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac
      841 gcatcacctc cgctgctcgc ggggatcctc tagagtcgac ggatccgggg aatcctgcag
      901 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
      961 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     1021 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     1081 cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg agaattcatg
     1141 ggcaccgggg gccggcgggg ggcggcggcg gcgccgctgc tggtggcggt ggccgcgctg
     1201 ctactgggcg ccgcgggcca cctgtacccc ggagaggtgt gtcccggcat ggatattgca
     1261 aaaattatca tcggccccct catctttgtc tttctcttca gtgttgtgat tggaagtatt
     1321 tatctattcc tgagaaagag gcagccagat gggccgctgg gaccgcttta cgcttcttca
     1381 aaccctgagt atctcagtgc cagtgatgtg tttccatgct ctgtgtacgt gccggacgag
     1441 tgggaggtgt ctcgagagaa gatcaccctc cttcgagagc tggggcaggg ctccttcggc
     1501 atggtgtatg agggcaatgc cagggacatc atcaagggtg aggcagagac ccgcgtggcg
     1561 gtgaagacgg tcaacgagtc agccagtctc cgagagcgga ttgagttcct caatgaggcc
     1621 tcggtcatga agggcttcac ctgccatcat gtggtgcgcc tcctgggagt ggtgtccaag
     1681 ggccagccca cgctggtggt gatggagctg atggctcacg gagacctgaa gagctacctc
     1741 cgttctctgc ggccagaggc tgagaataat cctggccgcc ctccccctac ccttcaagag
     1801 atgattcaga tggcggcaga gattgctgac gggatggcct acctgaacgc caagaagttt
     1861 gtgcatcggg acctggcagc gagaaactgc atggtcgccc atgattttac tgtcaaaatt
     1921 ggagactttg gaatgaccag agacatctat gaaacggatt actaccggaa agggggcaag
     1981 ggtctgctcc ctgtacggtg gatggcaccg gagtccctga aggatggggt cttcaccact
     2041 tcttctgaca tgtggtcctt tggcgtggtc ctttgggaaa tcaccagctt ggcagaacag
     2101 ccttaccaag gcctgtctaa tgaacaggtg ttgaaatttg tcatggatgg agggtatctg
     2161 gatcaacccg acaactgtcc agagagagtc actgacctca tgcgcatgtg ctggcaattc
     2221 aaccccaaga tgaggccaac cttcctggag attgtcaacc tgctcaagga cgacctgcac
     2281 cccagctttc cagaggtgtc gttcttccac agcgaggaga acaaggctcc cgagagtgag
     2341 gagctggaga tggagtttga ggacatggag aatgtgcccc tggaccgttc ctcgcactgt
     2401 cagagggagg aggcgggggg ccgggatgga gggtcctcgc tgggtttcaa gcggagctac
     2461 gaggaacaca tcccttacac acacatgaac ggaggcaaga aaaacgggcg gattctgacc
     2521 ttgcctcggt ccaatccttc ctacccatac gatgttccag attacgctta ggcggccgct
     2581 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2641 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2701 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2761 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     2821 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     2881 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2941 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3001 cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3061 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3121 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3181 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3241 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3301 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3361 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3421 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3481 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3541 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3601 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     3661 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3721 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3781 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3841 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3901 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3961 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4021 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4081 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4141 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4201 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4261 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4321 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4381 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4441 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4501 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4561 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4621 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4681 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4741 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4801 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4861 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4921 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4981 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5041 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5101 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5161 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5221 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5281 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5341 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5401 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     5461 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5521 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5581 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     5641 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     5701 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     5761 ataacaattt cacacaggaa acagctatga ccatgattac gccagattta attaagg
//
