LOCUS       sequence-473115-       10019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10019)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV_53BP1DeltaMFF_FLAG
FEATURES             Location/Qualifiers
     source          1..10019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        135..514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     686..705
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        780..884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(885..953,954..1745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1103..1122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1916..2504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2405..2424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2658..2675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2792..2813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2828..2833,2834..2851,2852..2858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2866..2882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2871..2893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2890..2906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2890..2906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2955..3076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2966..2985)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3020..3039
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3250..4044)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3361..3380)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3971..3990
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4111..4440)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4125..4260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4179..4198)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4425..4445
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4454..4882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4564..4585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4776..4795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4928..5152
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5141..5158
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(9684..9707)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(9744..9767)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(9783..9806)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9822..9845)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9886..9905)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9887..9905)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(9950..10019,1..134))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9979..9999)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
       61 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      121 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      181 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      241 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      301 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
      361 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      421 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      481 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
      541 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
      601 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
      661 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
      721 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
      781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2701 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2761 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2821 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2881 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg
     2941 gtatacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3001 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3061 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     3121 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     3181 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     3241 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     3301 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     3361 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     3421 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     3481 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     3541 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     3601 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     3661 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     3721 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     3781 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     3841 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     3901 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     3961 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     4021 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatcc
     4081 gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc
     4141 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     4201 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     4261 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     4321 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     4381 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     4441 attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4861 aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt tctttccgcc
     4921 tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc
     4981 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
     5041 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
     5101 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
     5161 gatcagcggg tttaaacggg ccctctagac tcgagttagt gagaaacata atcgtgttta
     5221 tattttggat gctgcttgaa tccaattctc tccccaacaa tgaggcactg gatcacccac
     5281 tcttgtgaca ccacaggcag ctgcaatgct tcagcacact tcagcaccga ggctgggcat
     5341 gaggggtccg tcaccaccac atcaaatacc cctaaagcaa tatctttgtt atgggcactt
     5401 gaatggtgct gcttcacaga ggctgcacca ccagtcatga ggatctcaga ccagagctcc
     5461 aggaagttct gctgttggtc tgataccaag agtaccttca gattctggaa aggattttca
     5521 cggggttgcc agtccagaat tctttgctcc tcaaggctgt acccagctgg caacagataa
     5581 ttacggtagt tctggagctg gttggcatgg caactatcat ggacccagac atgagacaca
     5641 caaggaatcc cactggcaag gcacaggaag tacttccggg ttcgacaatg ctgatccgca
     5701 attagaagac actggtaagc tgtgttacac tgggcttcat tgaaatcttc aaggatatag
     5761 ccagctcctg ctcgaagctg ggattctgta tactgcttgt tgaaaggagg aatttccaaa
     5821 aattcctcct cttcttcact gcttcctgta ggaccatctg gcagtttgga gcggctggcc
     5881 aacttgtcac tggttgtggc catggtaagg agaaatgcgt agcccagaaa caaggtcttg
     5941 ttgagaggca aaggccctct ctgctcttcc agggcgctat ggagcgactc tgtatcatcc
     6001 ccaggagcac tgactggttt ctcaccactc cccctctcag tctgaactgt ggcatcttgc
     6061 tttccaaagt tctggtccac tgtactggtc ccctgctcac acacattctt tatagtctgg
     6121 gttgctgctg aaacagtttc tgggaccctc aaggaacact ccatggtttg gattcctatg
     6181 ttattttggc tgggcctttc aatcaaggcc ttactaggat tttccttatt agtactccgt
     6241 ccttctttct ggtcttctag cacatctgtc accattgcct ctccttgagg actggatggc
     6301 ccttgtatga ccatcttctg ggaagcagga gaaacagggt ccttgacagg actaatgggc
     6361 ttcataggct gttgactctg cctgattgta tggtcaccct ccaatttttc tttctcctct
     6421 tctccttgag aacttggaaa gtggagcaag ttccccctga tgggtgtggt ggggggatcc
     6481 tcacttcgag cctcattctc ttgcctggct tcacagatac agctcaacac agacatggtc
     6541 ttctggggac tggcaacaga ctcagcaaca gcagtagatc cattttttct ttcaccagtt
     6601 gcaggctttt ccaggttgaa ctgcaaagac tcttcactcg cctcagtctc aggactaacc
     6661 agtttcattc ttagacataa tttatcatcc acgtctgggg cagccccaga atcccctttt
     6721 ccactcccaa gtatgggatc atgggtctcc accatgcttt cagacatgac atcactggtt
     6781 gctatggtgc tttctggata gttgctaata ccaataggag tactgtgtct cttgggctcc
     6841 aattttaggt gcccaataag aggtggggtt gcaccagtca atggtgggat gatatcacct
     6901 tctttaggca aagtgaaatg aaatggggtt tcactagaac tttcacagaa gctttgtgag
     6961 gcatgggcag agggcttccc cagcttctgg gcctctgcat ctgagcttag ctgctttgca
     7021 ttagccattt ttgagtcttc tgttaatgaa ttacttgttt tctcctgagt ttggggctgc
     7081 tgcaactcct ggtcaagtct taaaggatca tctgctctca ctaaaggtaa ggaaggctgt
     7141 gaagaatctt gctctacagg ttctgtttct gcagtccctg atttcagatc tccttcatat
     7201 tctacactct tttcttcctt ggtgtctaat ctattctcag cacatggttc tatttctgga
     7261 gcaatatcct cccatgactg ggaatctgag cacttctcca ctccctccaa aggttcacaa
     7321 gaaacatcaa ctctgggaga tggctctttc acatctacaa tgacaacact ggagtcctct
     7381 gaagtagctt cttcccaagc ttcctgttcc ttatgttcca attcttggtc aagtattgcc
     7441 aacttttgag gggaatcaat actaatcaca ctggtttcaa cttccatagc ttctgagcat
     7501 tctttttttg gcatttcctt ttgaagacac aacccttggg actgagtttc agtcagagaa
     7561 aggtgcaacg gaacactctc catattttct tctttgagtt cctctccttg actttcacaa
     7621 ggtgtctcag ggatttcttc cacctcagac cctgaagacc cctcctctgg atggtgttct
     7681 ttaatttcca tagcttcctc ctgatctaac acactagaaa gtgcctcaga tcgagtagct
     7741 ggtgacggaa ctgcctgact ccccgaatca caagtgagat caatacaaac atcttctgct
     7801 accgtttctt ctctgccctt gcaaccagtg gctaaaatac taatgtcatc cctggtgtct
     7861 gtatcatctc cctttgtttt gtcatcattc tgactcagtt gtacttcacc atcctgtgct
     7921 ggattcatca ggatactatc attttcagca ggaacaaatt tagaattgag aactggagac
     7981 atgggttccg tatcctcaat ctgtgtgttt tctccatctt catcaattct gtgagaactc
     8041 aagctctcca tctttgggga ctgactatat tcacttgtag aaagcatcaa cttgcaagaa
     8101 tcccctgtca aagatagccc aagatcctca ggggaattct ttggttcaat ctctgaagtt
     8161 ttagaacact caactgtcaa agatgaacta tgcatatctc catctttctt cccatcattt
     8221 gattgttctt ccagacttgg ggaaggaata aaaatgtcat gagaaaactg aggctgggat
     8281 gggataggaa gtgacccagg agggaagact ggtgtgctct gagatattgg tgttgaggct
     8341 tgtggtgata cagtggactc aggcaggaga ggggggtttt ctaactccac tggttcacca
     8401 ctttgaagtt tcttctgaaa aggctctcct ccttcttcag ataacactga cgtgtccatt
     8461 ggcttatctt gtctcccttc ttgctctgtg ggactgctag gaacgataaa aggagtagat
     8521 cggaaagcat caggagaagg agcaacaaga tctgaagaat tcgtggaaag actgtcctga
     8581 acaagggacc tctgaccaga gagctgcagg agatgcagag tggtggcagg agtggaagcc
     8641 aaagaacacc caccttcctc ccttgaagga gtagagcaac catcagaaga tactgtttta
     8701 ttgctctggt caaacaagtc ttcctgagtt gacaaaacct caggctctgg tgacttctga
     8761 atctgcagtc cactttccat aagttcttgt gcagacaact gttcttttgc ttccatagca
     8821 gcaacagatg ctttggggct aaaaggcatg tcctctgacc ttgcaggtgg tggattttgc
     8881 tcttttacaa catgtacatc cttactgggc tgtgctgtca cagggatgtc cttgctggac
     8941 tgttctgcta tggggatatc ttcgttggac tgttcttcat gcttaattgc agtattagca
     9001 tccacatcag acagcctggt ataaccagag ttggtggtta ctgattgtag ctgctctttg
     9061 tccacttcat atggcacagt attttcctca acatcctggc tctgggagag ttccagaacc
     9121 ccaaaaccca actgtgatga ggcagtatct tcagcaccaa gggaatgtgt agtattgcct
     9181 gaagtatctt cttccttctc tttctccttc tgttccaact cttctccctt ctcttcctcc
     9241 acagcaggag cagattccac tgacattccc agaacactgc ttgtcctgtt tggctgaggt
     9301 aactgctcaa tgacctgact gatggaacca catgtgtcca agttagaaga atccacaggg
     9361 tctgcaacct tgttttcttt caaatgttca ttgaacccac tattaccgtc tcctcgttct
     9421 tctccagctg tttgttcagg attggacaca acatccaaca caggattttc tttgtgcgtc
     9481 tggagattag gaaggtgtcg agatagcata ctgaagtgag aaccagaatc atcctctaga
     9541 acctggcttt caggctgaga atcttcaatt atcaggcaag gagtatcttg ctgagagaaa
     9601 tctgaatcca actgacttcc agtagggtcc attgtcgacc gaattcgggc ctccatggcc
     9661 ataagcatgg tggatccagc tcctttctcg aactgcgggt ggctccagct tcctcctccg
     9721 ctgcctccgc cgcttcctcc tcccttctcg aactgagggt ggctccagct ggcggccgac
     9781 cctttatcat catcatcttt ataatccata gatcctggta ccttgtcatc gtcgtccttg
     9841 tagtccatgg taagcttaag tttaaacgct agccagcttg ggtctcccta tagtgagtcg
     9901 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
     9961 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgtta
//
