LOCUS       sequence-473142-       11472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11472)
  TITLE     GMCL1 Controls 53BP1 Stability and Modulates Paclitaxel Sensitivity 
            in Cancer
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV_53BP1_FLAG
FEATURES             Location/Qualifiers
     source          1..11472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      375..384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(430..453)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(469..492)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(593..612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(657..860)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(686..706)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        861..1240
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1412..1431
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1506..1610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1611..1679,1680..2471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1829..1848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2642..3230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3131..3150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3384..3401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3518..3539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3554..3559,3560..3577,3578..3584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3592..3608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3597..3619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3616..3632
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3616..3632
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3681..3802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3692..3711)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3746..3765
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3976..4770)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4087..4106)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4697..4716
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4837..5166)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4851..4986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4905..4924)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5151..5171
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5180..5608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5290..5311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5502..5521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5654..5878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5867..5884
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        5904..5922
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5904..5921
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttccactgac attcccagaa cactgcttgt cctgtttggc tgaggtaact gctcaatgac
       61 ctgactgatg gaaccacatg tgtccaagtt agaagaatcc acagggtctg caaccttgtt
      121 ttctttcaaa tgttcattga acccactatt accgtctcct cgttcttctc cagctgtttg
      181 ttcaggattg gacacaacat ccaacacagg attttctttg tgcgtctgga gattaggaag
      241 gtgtcgagat agcatactga agtgagaacc agaatcatcc tctagaacct ggctttcagg
      301 ctgagaatct tcaattatca ggcaaggagt atcttgctga gagaaatctg aatccaactg
      361 acttccagta gggtccatgg tggcggaacc accaccaccg gaaccaccac caccggaacc
      421 accaccacct ttatcatcat catctttata atccccagat cctggtacct tgtcatcgtc
      481 gtccttgtag tccatggtct cgagcggccg ccagtgtgat ggatatctgc agaattccag
      541 cacactggcg gccgttacta gtggatccga gctcggtacc aagcttgggt ctccctatag
      601 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      661 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      721 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      781 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      841 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
      901 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
      961 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     1021 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     1081 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     1141 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     1201 gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt
     1261 acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc
     1321 aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca
     1381 caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     1441 caccataggg gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa
     1501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3421 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3481 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     3541 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     3601 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg
     3661 tcgacggtat acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3721 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3781 tataagctgc aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct
     3841 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     3901 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     3961 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     4021 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     4081 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     4141 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     4201 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     4261 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     4321 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     4381 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     4441 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     4501 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     4561 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     4621 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     4681 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     4741 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     4801 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
     4861 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     4921 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     4981 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     5041 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     5101 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     5161 acacacattc cacagaatta attcgcgtta aatttttgtt aaatcagctc attttttaac
     5221 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     5281 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     5341 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     5401 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     5461 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     5521 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     5581 gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
     5641 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     5701 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     5761 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     5821 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     5881 gaggctgatc agcgagctct agcatttagg tgacactata gaatagggcc cttagtgaga
     5941 aacataatcg tgtttatatt ttggatgctg cttgaatcca attctctccc caacaatgag
     6001 gcactggatc acccactctt gtgacaccac aggcagctgc aatgcttcag cacacttcag
     6061 caccgaggct gggcatgagg ggtccgtcac caccacatca aataccccta aagcaatatc
     6121 tttgttatgg gcacttgaat ggtgctgctt cacagaggct gcaccaccag tcatgaggat
     6181 ctcagaccag agctccagga agttctgctg ttggtctgat accaagagta ccttcagatt
     6241 ctggaaagga ttttcacggg gttgccagtc cagaattctt tgctcctcaa ggctgtaccc
     6301 agctggcaac agataattac ggtagttctg gagctggttg gcatggcaac tatcatggac
     6361 ccagacatga gacacacaag gaatcccact ggcaaggcac aggaagtact tccgggttcg
     6421 acaatgctga tccgcaatta gaagacactg gtaagctgtg ttacactggg cttcattgaa
     6481 atcttcaagg atatagccag ctcctgctcg aagctgggat tctgtatact gcttgttgaa
     6541 aggaggaatt tccaaaaatt cctcctcttc ttcactgctt cctgtaggac catctggcag
     6601 tttggagcgg ctggccaact tgtcactggt tgtggccatg gtaaggagaa atgcgtagcc
     6661 cagaaacaag gtcttgttga gaggcaaagg ccctctctgc tcttccaggg cagagggttc
     6721 accggtgttg tctccactct cacaggggct cacaaactct cctgccccta ctgcaccagg
     6781 ttttactgtg gcagacttgc gacctcgctt ggcaggggac cgttcctctt cagaagtgat
     6841 aagttttctt ttgcctgaga gaactcccat ggaggcacga ggactttctg tgatctttcg
     6901 ggtaggggtt gtgctgctgc tactggaggc agtaggggtg gctggggagc tgacgttact
     6961 gcgccgtttc cgcttccctt ccaccaaatt gtctaagctg atatctgctg cctttgtaag
     7021 aggtgttact gcttcatagg ggccaagccc atactgctct ctcagtctgt ttccttgctc
     7081 caaggacagg atgacagcca ttcgcttata ccactttctt tggccttctt tttcaatgct
     7141 gtagtacagt tccccagact ccttcctatg tcctttcacc actcctgcac tgaaatactc
     7201 atcctccgag agggccgtca cttcagtgtc cagcgggatg gggtcacata acagaatgtc
     7261 tttgcccaac acatcacatt cgtacccatc atcaaagagc aatttatact tcccagctcc
     7321 gacatctcgt gtgattttcc cagagtaaaa gtagccattg gatgaccact tggctacaac
     7381 acggagccct acaaagctat ttcctggaga ggaggcatct aagccatcag aagggccagc
     7441 agcagcctgg aaaggaattt ctggagagtc actacgacgc aaagcaccag cacccacatc
     7501 tgttcgtctg gtggagtctg gcactcgggg cacgacacgg ctgaaggatt tatcatctgg
     7561 tgacaagtta ggtgaaatgt cctctatgcc caaggggcca ggcacagctg tttctctggt
     7621 tccagtggtc cgagaaggtg ggcggcccct tcgcccacgc ccacgaggcg tgactggagc
     7681 cttccctccc tgtctgatgc caaggcctgc atcaccatcc tcctcacaca ctggcgtccc
     7741 tgtctgactg accccttttc taggactcag ttttcctggg cctcctcggg agctgggtaa
     7801 ggcaaaatct gcgggttctg tcccgctggt tttccctctg agtggtccgg ctcctttccc
     7861 tgagcttcca ctgctgtgca tagctgagag acttgtccca cttgatgtgc ggtgtaagct
     7921 ggatgccttg gaggagaagg agctgatatc ccccaggtca cctgaggagc ccccagtctg
     7981 tgaaggggaa acttcagttt cacactcctg acactctaca attggctctt cagtctcctc
     8041 agttactttt ctttctactt ctgttccatc cacataatac acatctgtaa tgacacgagt
     8101 gacaagtgtg cgtacttccc ggattgttct catgtgacga tgtaagacat ggccatgtgg
     8161 aggctgaggc atatcaaact cttcttctcc ctggctatgg agcgactctg tatcatcccc
     8221 aggagcactg actggtttct caccactccc cctctcagtc tgaactgtgg catcttgctt
     8281 tccaaagttc tggtccactg tactggtccc ctgctcacac acattcttta tagtctgggt
     8341 tgctgctgaa acagtttctg ggaccctcaa ggaacactcc atggtttgga ttcctatgtt
     8401 attttggctg ggcctttcaa tcaaggcctt actaggattt tccttattag tactccgtcc
     8461 ttctttctgg tcttctagca catctgtcac cattgcctct ccttgaggac tggatggccc
     8521 ttgtatgacc atcttctggg aagcaggaga aacagggtcc ttgacaggac taatgggctt
     8581 cataggctgt tgactctgcc tgattgtatg gtcaccctcc aatttttctt tctcctcttc
     8641 tccttgagaa cttggaaagt ggagcaagtt ccccctgatg ggtgtggtgg ggggatcctc
     8701 acttcgagcc tcattctctt gcctggcttc acagatacag ctcaacacag acatggtctt
     8761 ctggggactg gcaacagact cagcaacagc agtagatcca ttttttcttt caccagttgc
     8821 aggcttttcc aggttgaact gcaaagactc ttcactcgcc tcagtctcag gactaaccag
     8881 tttcattctt agacataatt tatcatccac gtctggggca gccccagaat ccccttttcc
     8941 actcccaagt atgggatcat gggtctccac catgctttca gacatgacat cactggttgc
     9001 tatggtgctt tctggatagt tgctaatacc aataggagta ctgtgtctct tgggctccaa
     9061 ttttaggtgc ccaataagag gtggggttgc accagtcaat ggtgggatga tatcaccttc
     9121 tttaggcaaa gtgaaatgaa atggggtttc actagaactt tcacagaagc tttgtgaggc
     9181 atgggcagag ggcttcccca gcttctgggc ctctgcatct gagcttagct gctttgcatt
     9241 agccattttt gagtcttctg ttaatgaatt acttgttttc tcctgagttt ggggctgctg
     9301 caactcctgg tcaagtctta aaggatcatc tgctctcact aaaggtaagg aaggctgtga
     9361 agaatcttgc tctacaggtt ctgtttctgc agtccctgat ttcagatctc cttcatattc
     9421 tacactcttt tcttccttgg tgtctaatct attctcagca catggttcta tttctggagc
     9481 aatatcctcc catgactggg aatctgagca cttctccact ccctccaaag gttcacaaga
     9541 aacatcaact ctgggagatg gctctttcac atctacaatg acaacactgg agtcctctga
     9601 agtagcttct tcccaagctt cctgttcctt atgttccaat tcttggtcaa gtattgccaa
     9661 cttttgaggg gaatcaatac taatcacact ggtttcaact tccatagctt ctgagcattc
     9721 tttttttggc atttcctttt gaagacacaa cccttgggac tgagtttcag tcagagaaag
     9781 gtgcaacgga acactctcca tattttcttc tttgagttcc tctccttgac tttcacaagg
     9841 tgtctcaggg atttcttcca cctcagaccc tgaagacccc tcctctggat ggtgttcttt
     9901 aatttccata gcttcctcct gatctaacac actagaaagt gcctcagatc gagtagctgg
     9961 tgacggaact gcctgactcc ccgaatcaca agtgagatca atacaaacat cttctgctac
    10021 cgtttcttct ctgcccttgc aaccagtggc taaaatacta atgtcatccc tggtgtctgt
    10081 atcatctccc tttgttttgt catcattctg actcagttgt acttcaccat cctgtgctgg
    10141 attcatcagg atactatcat tttcagcagg aacaaattta gaattgagaa ctggagacat
    10201 gggttccgta tcctcaatct gtgtgttttc tccatcttca tcaattctgt gagaactcaa
    10261 gctctccatc tttggggact gactatattc acttgtagaa agcatcaact tgcaagaatc
    10321 ccctgtcaaa gatagcccaa gatcctcagg ggaattcttt ggttcaatct ctgaagtttt
    10381 agaacactca actgtcaaag atgaactatg catatctcca tctttcttcc catcatttga
    10441 ttgttcttcc agacttgggg aaggaataaa aatgtcatga gaaaactgag gctgggatgg
    10501 gataggaagt gacccaggag ggaagactgg tgtgctctga gatattggtg ttgaggcttg
    10561 tggtgataca gtggactcag gcaggagagg ggggttttct aactccactg gttcaccact
    10621 ttgaagtttc ttctgaaaag gctctcctcc ttcttcagat aacactgacg tgtccattgg
    10681 cttatcttgt ctcccttctt gctctgtggg actgctagga acgataaaag gagtagatcg
    10741 gaaagcatca ggagaaggag caacaagatc tgaagaattc gtggaaagac tgtcctgaac
    10801 aagggacctc tgaccagaga gctgcaggag atgcagagtg gtggcaggag tggaagccaa
    10861 agaacaccca ccttcctccc ttgaaggagt agagcaacca tcagaagata ctgttttatt
    10921 gctctggtca aacaagtctt cctgagttga caaaacctca ggctctggtg acttctgaat
    10981 ctgcagtcca ctttccataa gttcttgtgc agacaactgt tcttttgctt ccatagcagc
    11041 aacagatgct ttggggctaa aaggcatgtc ctctgacctt gcaggtggtg gattttgctc
    11101 ttttacaaca tgtacatcct tactgggctg tgctgtcaca gggatgtcct tgctggactg
    11161 ttctgctatg gggatatctt cgttggactg ttcttcatgc ttaattgcag tattagcatc
    11221 cacatcagac agcctggtat aaccagagtt ggtggttact gattgtagct gctctttgtc
    11281 cacttcatat ggcacagtat tttcctcaac atcctggctc tgggagagtt ccagaacccc
    11341 aaaacccaac tgtgatgagg cagtatcttc agcaccaagg gaatgtgtag tattgcctga
    11401 agtatcttct tccttctctt tctccttctg ttccaactct tctcccttct cttcctccac
    11461 agcaggagca ga
//
