LOCUS       sequence-47317-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Manchanda PK, Jones GN, Lee AA, Pringle DR, Zhang M, Yu L, La Perle 
            KM, Kirschner LS
  TITLE     Rac1 is required for Prkar1a-mediated Nf2 suppression in Schwann 
            cell tumors.
  JOURNAL   Oncogene. 2013 Jul 25;32(30):3491-9. doi: 10.1038/onc.2012.374. Epub
            2012 Oct 8.
  PUBMED    23045281
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2012.374"; journalName: "Oncogene"; date: "2013-07-25- 
            25"; volume: "32"; issue: "30"; pages: "3491-9"
COMMENT     Sequence Label: pRK5myc Rac1 wt
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             60..89
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             99..674
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="QAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMV
                     DGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHC
                     PNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRG
                     LKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     regulatory      694..703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    707..841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(744..763)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccggtgtcac tcccaggtcc aactgcacct cggttctatc gattgaattg gccaccatgg
       61 agcagaagct gatctccgag gaggacctgg gatccccgca ggccatcaag tgtgtggtgg
      121 tgggagacgg agctgtaggt aaaacttgcc tactgatcag ttacacaacc aatgcatttc
      181 ctggagaata tatccctact gtctttgaca attattctgc caatgttatg gtagatggaa
      241 aaccggtgaa tctgggctta tgggatacag ctggacaaga agattatgac agattacgcc
      301 ccctatccta tccgcaaaca gatgtgttct taatttgctt ttcccttgtg agtcctgcat
      361 catttgaaaa tgtccgtgca aagtggtatc ctgaggtgcg gcaccactgt cccaacactc
      421 ccatcatcct agtgggaact aaacttgatc ttagggatga taaagacacg atcgagaaac
      481 tgaaggagaa gaagctgact cccatcacct atccgcaggg tctagccatg gctaaggaga
      541 ttggtgccgt aaaatacctg gagtgctcgg cgctcacaca gcgaggcctc aagacagtgt
      601 ttgacgaagc gatccgagca gtcctctgcc cgcctcccgt gaagaagagg aagagaaaat
      661 gcctgctgtt gtaagaattc ctgcagaagc ttggccgcca tggcccaact tgtttattgc
      721 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      781 ttcactgcat tctagttgtg gtttgtccna actcatcaat gtatcttatc atgtctggat
      841 cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaagagg aacttggtta
      901 ggtac
//
