LOCUS       sequence-47320-e        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL600
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..139
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    185..203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      602..688
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    743..759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggggnnnnca ttttgaatgt atttannaaa antaancaaa tagggnttcc gcgcacattt
       61 ccccgaaaag tgccncctga cgtntaagaa accattatta tcatgacatt aacctataaa
      121 aataggcgta tcacgaggcc ctttcgtctt cacctcgagt ccctatcagt gatagagatt
      181 gacatcccta tcagtgatag agatactgtc gacgagtcag acaacgaagt gaacatcagg
      241 atgatgacac ttctgcagga cacaccagga tggtgtttca gggaaaggct tctggatgaa
      301 gcgaagagga tgacgcagga cgcgttaaag gacacctcca ggatggagaa tgagaaccgg
      361 tcaggatgat tcggtgggtc aggaaggcca gggacacttc aggatgaagt atcacatcgg
      421 ggtggtgtga gcaggaagca atagttcagg atgaacgatt ggccgcaagg ccagaggaaa
      481 agttgtcaag gatgagcagg gagcaacaaa agtagctgga atgctgcgaa acgaaccggg
      541 agcgctgtga atacagtgct cccttttttt attgggccca tggtacgcgt gctagaggca
      601 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      661 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acctaggcgc ggatccataa
      721 atgtgagcgg ataacattga cattgtgagc ggataacaag atactcccgg gtaaggagga
      781 aaaaaaatgc tgattctgac tcgtcgagtt ggtgagaccc tcatgattgg ggatgaggtc
      841 accgtgacag ttttaggggt aaagggcaac caggtacgta ttggcgtaaa tgccccgaag
      901 gaagtttctg ttcaccgtga agagatctac cagcgtatcc aggctgaaaa atcccagcng
      961 tccagttact aaggtacctg aaagaagctt taatcgtaca gggtagtaca aataaaaagc
     1021 actcagta
//
