LOCUS       sequence-47321-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL601
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     78..96
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      483..569
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gggttccgcg cacatttccc cgaaaannnn nncctgacgt ctaagaaacc attattatca
       61 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagtccc
      121 tatcagtgat agagattgac atccctatca gtgatagaga tactgtcgac atagagcgag
      181 gacgctaaca ggaacaatga ctcaggatga gggtcaggag cgccaggagg cgaagacaga
      241 ggattgtcag gaagacaaac gtccggagac gtaattaaac ggaaatggaa tcaacacgga
      301 ttgttccggc taaaggaaaa acagggtgtg ttggcggcct gcaaggattg taagacccgt
      361 taagggttat gagtcaggaa aaaaggcgac agagtaatct gtcgcctttt ttctttgctt
      421 gctttctgtt agattccgcc gcaaatctat actggggccc atggtacgcg tgctagaggc
      481 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      541 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctaggcg cggatccata
      601 aatgtgagcg gataacattg acattgtgag cggataacaa gatactcccg ggtaaggagg
      661 aaaaaaaatg ctgattctga ctcgtcgagt tggtgagacc ctcatgattg gggatgaggt
      721 caccgtgaca gttttagggg taaagggcaa ccaggtacgt attggcgtaa atgccccgaa
      781 ggaagtttct gttcaccgtg aagagatcta ccagcgtatc cnggctgaaa aatcccagca
      841 gtccagttac taangtacct gaaagaagct ttaatcgtac agggtagtac aaataaaaaa
      901 ggnannnnnn atgacgtgcc ttttttcttg tnnnnnnnna atnncng
//
