LOCUS       sequence-473225-       12205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NUP98::NSD1 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Mohanty S, Charles Cano F, Gabdoulline R, Lai CK, Othman B, 
            Sudarsanam H, Eder T, Grebien F, Lipka DB, Henschler R, Heuser M
  TITLE     Clec12a is required for the pathogenesis of NUP98::NSD1 AML.
  JOURNAL   Blood Adv. 2025 May 7:bloodadvances.2024015739. doi: 
            10.1182/bloodadvances.2024015739.
  PUBMED    40334081
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood Adv. 
            2025 May 7:bloodadvances.2024015739. doi: 
            10.1182/bloodadvances.2024015739."
COMMENT     Sequence Label: MSCV-IRES-NUP98::NSD1-GFP
FEATURES             Location/Qualifiers
     source          1..12205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..921
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             988..1404
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1334..1356
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1372..1388
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    7197..7764
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7214..7766
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7358..7375)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7585..7604
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7778..7780,7781..7783,7784..8497)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7823..7844)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8084..8103)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8431..8452
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8599..9113
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    9275..9291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9299..9305,9306..9323,9324..9329))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9344..9365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9482..9499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9653..10241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9733..9752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10412..11203,11204..11272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11035..11054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11273..11377)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11445..11463
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11501..11523)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11623..11642
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11836..11858
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11986..12005
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(12205,1..516)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
       61 tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc
      121 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
      181 aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt
      241 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
      301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
      361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat
      421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc
      481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag
      541 acccctgccc agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc
      601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg
      661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
      721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
      781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
      841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
      901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
      961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1381 gccctcactc cttctctagg cgccggaatt agatctctcg aggttaacga attccggtgc
     1441 caccatgttt aacaaatcat ttggaacacc ctttgggggt ggcacaggtg gctttggcac
     1501 aacttcaaca tttggacaga atactggctt tggcactact agtggagggg catttggaac
     1561 atctgcattt ggttctagca acaatactgg aggcctcttt ggaaattcac agactaaacc
     1621 aggaggattg tttggaacca gttcatttag ccagccagct acctccacaa gcaccggctt
     1681 tgggtttggt acgtcaacag gaacagcaaa taccttgttt ggaactgcaa gcacagggac
     1741 cagtctcttc tcatcccaaa acaatgcctt tgcacaaaat aaaccaactg gctttggcaa
     1801 ttttggaacc agtactagca gtggaggact ctttggaacc acaaatacca cctctaatcc
     1861 ttttggcagc acatctggct ccctctttgg gccaagtagt tttacagctg ctcctactgg
     1921 gactactatt aaatttaacc ctccaactgg tacagatact atggtcaaag ctggagttag
     1981 cactaacata agtaccaagc accagtgtat tactgctatg aaagaatatg aaagcaagtc
     2041 actagaggaa cttcgtttag aggattatca ggctaacagg aagggcccac agaaccaggt
     2101 gggagcaggt accacaactg gcttgtttgg gtcttctcca gccacttcca gcgcaacagg
     2161 actcttcagc tcctccacca ctaattcagg ctttgcatat ggtcagaaca aaactgcctt
     2221 tggaactagt acaactggat ttggaacaaa tccaggtggt ctctttggcc aacagaatca
     2281 gcagactacc agcctcttca gcaaaccatt tggccaggct acaaccaccc agaacactgg
     2341 cttttccttt ggtaatacca gcaccatagg acagccaagc accaacacca tgggattatt
     2401 tggagtaacc caagcctcac agcctggagg tctttttggg acagctacaa acaccagcac
     2461 tgggacagca tttggaacag gaacaggtct ctttgggcag accaatactg gatttggtgc
     2521 tgttggttcg accctgtttg gcaataacaa gcttactaca tttggaagca gcacaaccag
     2581 tgcaccttca tttggtacaa ccagtggcgg gctctttggt tttggcacaa ataccagtgg
     2641 gaatagtatt tttggaagta aaccagcacc tgggactctt ggaactgggc ttggtgcagg
     2701 atttggaaca gctcttggtg ctggacaggc atctttgttt gggaacaacc aacctaagat
     2761 tggagggcct cttggtacag gagcctttgg ggcccctgga tttaatacta cgacagccac
     2821 tttgggcttt ggagcccccc aggccccagt agctgctgtg cggtcagaga agaaacgcct
     2881 taggaagcca agcaagtggc ttttggaata tacagaagaa tatgatcaga tatttgctcc
     2941 taagaaaaaa caaaagaagg tacaggagca ggtgcacaag gtaagttccc gctgtgaaga
     3001 ggaaagcctt ctagcccgag gtcgatctag tgctcagaac aagcaggtgg acgagaattc
     3061 tttgatttca accaaagaag agcctccagt tcttgaaagg gaggctccgt ttttggaggg
     3121 ccccttggct cagtcagaac ttggaggtgg acatgctgag ttgccgcagc tgaccttgtc
     3181 tgtgcctgtg gctccggaag tctctccacg gcctgccctt gagtctgagg aattgctagt
     3241 taaaacgcca ggaaattatg aaagtaaacg tcaaagaaaa ccaactaaga aacttcttga
     3301 atccaatgat ttagaccctg gatttatgcc caagaagggg gaccttggcc tttctaaaaa
     3361 gtgctatgaa gctggtcacc tggagaatgg cataactgaa tcttgtgcca catcttattc
     3421 aaaagatttt ggtggaggca ctaccaagat atttgacaag ccaaggaagc gaaaacgaca
     3481 gaggcatgct gcagccaaga tgcagtgtaa aaaagtgaaa aatgatgact cgtcaaaaga
     3541 gattccaggc tcagagggag aactaatgcc tcacaggacg gccacaagcc ccaaggagac
     3601 tgttgaggaa ggtgtagaac acgatcccgg gatgcctgcc tctaaaaaaa tgcagggtga
     3661 acgcggtgga ggagctgcac tcaaggagaa tgtctgtcag aattgtgaaa aattgggtga
     3721 gctgctgtta tgtgaggctc agtgctgtgg ggctttccac ctggagtgcc ttggattgac
     3781 tgagatgcca agaggaaaat ttatctgcaa tgaatgtcgc acaggaatcc atacctgttt
     3841 tgtatgtaag cagagtgggg aagatgttaa aaggtgcctt ctacccttgt gtggaaagtt
     3901 ttaccatgaa gagtgtgtcc agaagtaccc acccactgtt atgcagaaca agggcttccg
     3961 gtgctccctc cacatctgta taacctgtca tgctgctaat ccagccaatg tttctgcatc
     4021 taaaggtcgg ttgatgcgct gtgtccgctg tcctgtggca taccacgcca atgacttttg
     4081 cctggctgct gggtcaaaga tccttgcatc taatagtatc atctgcccta atcactttac
     4141 ccctaggcgg ggctgccgaa atcatgagca tgttaatgtt agctggtgct ttgtgtgctc
     4201 agaaggaggc agccttctgt gctgtgattc ttgccctgct gcttttcatc gtgaatgcct
     4261 gaacattgat atccctgaag gaaactggta ttgcaatgac tgtaaagcag gcaaaaagcc
     4321 acactacagg gagattgtct gggtaaaagt tggacgatac aggtggtggc cagctgagat
     4381 ctgccatcct cgagctgttc cttccaacat tgataagatg agacatgatg tgggagagtt
     4441 cccagtcctc ttttttggat ctaatgacta tttgtggact caccaggccc gagtcttccc
     4501 ttacatggag ggtgacgtga gcagcaagga taagatgggc aaaggagtgg atgggacata
     4561 taaaaaagct cttcaggaag ctgcagcaag gtttgaggaa ttaaaggccc aaaaagagct
     4621 aagacagctg caggaagacc gaaagaatga caagaagcca ccaccttata aacatataaa
     4681 ggtaaaccgt cctattggca gggtacagat cttcactgca gacttatctg aaataccccg
     4741 ttgcaactgt aaagctactg atgagaaccc ctgtgggata gactctgaat gcatcaaccg
     4801 catgctgctc tatgagtgcc accccacagt gtgtcctgcc ggagggcgct gtcaaaacca
     4861 gtgcttttcc aagcgccaat atccagaggt tgaaattttc cgcacattac agcggggttg
     4921 gggtctacgg acaaaaacag atattaaaaa gggtgaattt gtgaatgagt atgtgggtga
     4981 gcttatagat gaagaagaat gcagagctcg aattcgctat gctcaagaac atgatatcac
     5041 taatttctat atgctcaccc tagacaaaga ccgaatcatt gatgctggtc ccaaaggaaa
     5101 ctatgctcgg ttcatgaatc attgctgcca gcccaactgt gaaacacaga agtggtctgt
     5161 gaatggagat acccgtgtag gcctttttgc actaagtgac attaaagcag gcactgaact
     5221 taccttcaac tacaacctag aatgtcttgg gaatggaaag actgtttgca aatgtggagc
     5281 cccgaactgc agtggcttct tgggtgtaag gccaaagaat caacccattg ccacggaaga
     5341 aaagtcaaag aaattcaaga agaagcaaca gggaaagcgc aggacccagg gtgaaatcac
     5401 aaaggagcga gaagatgagt gttttagttg tggggatgct ggccagctcg tctcctgcaa
     5461 gaaaccaggc tgcccaaaag tttaccacgc agactgtctc aatctgacca agcgaccagc
     5521 agggaaatgg gaatgtccgt ggcatcagtg tgacatctgc gggaaggaag cagcctcctt
     5581 ctgtgagatg tgccccagct ccttttgtaa gcagcatcga gaagggatgc ttttcatttc
     5641 caaactggat gggcgtctgt cttgtactga gcatgacccc tgtgggccca atcctctgga
     5701 acctggggag atccgtgagt atgtgcctcc cccagtaccg ctgcctccag ggccaagcac
     5761 tcacctggca gagcaatcaa caggaatggc tgctcaggca cccaaaatgt cagataaacc
     5821 tcctgctgac accaaccaga tgctgtcgct ctccaaaaaa gctctggcag ggacttgtca
     5881 gaggccattg ctacctgaaa gacctcttga gagaactgac tccaggcccc agcctttaga
     5941 taaggtcaga gacctcgctg ggtcagggac caaatcccaa tccttggttt ccagccagag
     6001 gccactggac aggccaccag cagtggcagg accaagaccc cagctaagcg acaaaccctc
     6061 tccagtgacc agcccaagct cctcaccctc agtcaggtcc caaccactgg aaagacctct
     6121 ggggacggct gacccaaggc tggataaatc cataggtgct gccagcccaa ggccccagtc
     6181 actggagaaa acctcagttc ccactggcct gagacttccg ccgccagaca gactgctcat
     6241 tactagcagt cccaaacccc agacttcaga caggcctact gacaaacccc atgcctcttt
     6301 gtcccagaga ctcccacctc ctgagaaagt actatcagct gtggtccaga cccttgtagc
     6361 taaagaaaaa gcactgaggc ctgtggacca gaatactcag tcaaaaaata gagctgcttt
     6421 ggtgatggat ctcatagacc taactcctcg ccagaaggag cgggcagctt cacctcatca
     6481 ggtcacacca caggctgatg agaagatgcc agtgttggag tcaagttcat ggcctgccag
     6541 caaaggtctg gggcatatgc cgagagctgt tgagaaaggc tgtgtgtcag atcctcttca
     6601 gacatctggg aaagcagcag ccccttcaga ggacccctgg caagctgtta aatcactcac
     6661 ccaggccaga cttctttctc agcctcctgc caaggccttt ttatatgagc caacaactca
     6721 ggcctcagga agagcttctg caggggctga gcagacccca gggcctctta gccaatcccc
     6781 gggcctggtg aagcaggcga agcagatggt cggaggccag caactacctg cacttgccgc
     6841 caagagtggg caatctttta ggtctctcgg gaaggcccca gcctccctcc ccactgaaga
     6901 aaagaagttg gtaaccacag agcaaagtcc ctgggccctg ggaaaagcct catcacgggc
     6961 agggctctgg cccatagtgg ctggacagac actggcacag tcttgctggt ctgctgggag
     7021 cacacagaca ttggcacaga cttgctggtc tcttggaaga gggcaagacc ccaaaccaga
     7081 gcaaaataca cttccagctc ttaaccaggc tccttccagt cacaagtgtg cagaatcaga
     7141 acagaagtag gtcgacctcg agcacggaca tcgacctgca gccaagctta tcgaattccg
     7201 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     7261 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     7321 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7381 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7441 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7501 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7561 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     7621 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     7681 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     7741 ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct
     7801 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     7861 cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     7921 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     7981 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8041 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8101 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8161 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     8221 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat
     8281 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8341 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     8401 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     8461 cgggatcact ctcggcatgg acgagctgta caagtaatga attaattaag aattatcaag
     8521 cttatcgata ccgtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt
     8581 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     8641 acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt
     8701 taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     8761 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct
     8821 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     8881 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     8941 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg
     9001 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     9061 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac
     9121 agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg
     9181 actcaattag ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg
     9241 gacagcgcag aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca
     9301 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9361 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9421 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9481 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9541 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9601 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9661 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9721 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9781 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9841 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9901 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9961 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10021 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10081 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10141 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10201 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10261 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10321 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10381 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10441 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10501 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10561 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10621 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10681 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10741 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10801 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10861 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10921 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10981 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11041 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11101 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11161 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11221 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11281 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11341 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11401 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    11461 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    11521 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    11581 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    11641 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    11701 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    11761 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    11821 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg
    11881 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
    11941 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
    12001 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
    12061 gaggcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
    12121 tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt
    12181 atttagtctc cagaaaaagg gggga
//
