LOCUS       sequence-47325-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pActinin-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..836)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(404..423)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      833..842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgcttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
      181 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgtcgacg
      241 atgtaggcgc cgggcaactg cacgggcttc ttggccttgt aggtggtctt gacctcggag
      301 gtgtagtggc cgccgtcctt cagcttcagc ctgagcttgt cctcgccctt cagggcgccg
      361 tcctcggggt acatccgctc ggaggaggcc tccatgccca tggtcttctt ctgcattacg
      421 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
      481 tgcagggagg agtcctgagt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      541 tcccacttga agccctcggg gaaggacagc ttgaggtagt cggggatgtc ggcggggtgc
      601 ttcacgtagg ccttggagcc gtaggtgaac tgaggggaca agatgtccca ggcgaagggc
      661 agggggccac ccttggtcac cttcagctta acggtctgaa agccctcgta ggggcggccc
      721 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg
      781 aagcgcatga actccttgat gatggccatg ttattctcct cgcccttgct caccatggtg
      841 gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
//
