LOCUS       sequence-47328-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pLSSmOrange-alpha-Tubulin
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..747
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     453..472
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggtttgtg aaccgtcaga tccgctagcg gtcgccacca tggtgagcaa gggcgaggag
       61 aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
      121 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggctttcag
      181 accgttaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcttgtcc
      241 cctcagttca cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      301 ctcaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      361 ggcgtggtga ccgtgactca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      421 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
      481 atggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgaggacaag
      541 ctcaggctga agctgaagga cggcggccac tacacctccg aggtcaagac cacctacaag
      601 gccaagaagc ccgtgcagtt gcccggcgcc tacatcgtcg acatcaagtt ggacatcacc
      661 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
      721 accggcggca tggacgagct gtacaagggt ggaggaggtt ccggactcag atctcgagtg
      781 cgtgagtgca tctccatcca cgttggccag gctggtgtcc agattggcaa tgcctgctgg
      841 gagctctact gcctggaaca cggcatccag cccgatggcc agatgccaag tgacaagacc
      901 attgggggag gagatgatct caactctata agg
//
