LOCUS       sequence-473285-        9163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Perturb Seq lentiviral sgRNA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Sivakumar S, Wang Y, Goetsch SC, Pandit V, Wang L, Zhao H, 
            Sundarrajan A, Armendariz D, Takeuchi C, Nzima M, Chen WC, Dederich 
            AE, El Hayek L, Gao T, Ghazawi R, Gogate A, Kaur K, Kim HB, McCoy 
            MK, Niederstrasser H, Oura S, Pinzon-Arteaga CA, Sanghvi M, Schmitz 
            DA, Yu L, Zhang Y, Zhou Q, Kraus WL, Xu L, Wu J, Posner BA, Chahrour
            MH, Hon GC, Munshi NV
  TITLE     Benchmarking and optimizing Perturb-seq in differentiating human 
            pluripotent stem cells.
  JOURNAL   bioRxiv [Preprint]. 2025 Jan 23:2025.01.21.633969. doi: 
            10.1101/2025.01.21.633969.
  PUBMED    39896670
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jan 23:2025.01.21.633969. doi: 
            10.1101/2025.01.21.633969."
COMMENT     Sequence Label: Lenti-U6-sgRNA-BFP-Puro
FEATURES             Location/Qualifiers
     source          1..9163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1713..1961
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1713..1733
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1884..1903
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2149..2266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2405..3583
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2636..3574
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3531..3551
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4325..4381
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4382..4978
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4875..4895
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5016..5604
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5069..5089)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5487..5498)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5676..5909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5987..6108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6024..6043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6078..6097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6148..6283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6303..6322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6304..6322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6332..6349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6332..6348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6341..6363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6490..6945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6577..6596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6787..6808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6971..7075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7076..7144,7145..7936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7294..7313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8107..8695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8596..8615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8849..8866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8983..9004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9019..9024,9025..9042,9043..9049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9057..9073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9062..9084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9081..9097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9081..9097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9116..9136
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9118..9136
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     1741 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     1801 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1861 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     1921 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacggg ataccgtctc
     1981 tgtttaagag ctaagctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     2041 cttggccgct ttaaggccgg tcctagcaag gccaagtggc accgagtcgg tgctttttta
     2101 agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2161 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2221 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2281 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     2341 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     2401 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     2461 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     2521 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     2581 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     2641 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2701 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2761 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2821 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2881 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2941 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     3001 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     3061 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     3121 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     3181 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     3241 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     3301 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     3361 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     3421 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     3481 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     3541 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgagagctcg gtaccgcggg
     3601 cccgggatcc accatggtct ccaagggtga ggaactgatc aaagaaaata tgcatatgaa
     3661 gctgtacatg gaggggaccg tggataacca ccacttcaag tgtactagtg agggcgaagg
     3721 caaaccatat gaaggtacgc agacaatgag aatcaaggtt gttgaaggcg gaccactgcc
     3781 ttttgccttt gacattcttg ctaccagttt cctttacggt tcaaaaacct ttataaacca
     3841 cacacagggg atccctgatt tcttcaagca gagctttcct gaagggttta cttgggagag
     3901 agtgactacg tatgaggacg gcggtgtcct gacagccacc caggatacat cacttcagga
     3961 cggctgtctt atttataatg tgaagatcag aggcgtgaat ttcacttcca acggccccgt
     4021 gatgcagaaa aaaacactcg gctgggaggc ctttacggaa acactttatc cagctgatgg
     4081 cggtctggag ggaagaaacg acatggcttt gaagctggtg gggggaagtc atctgattgc
     4141 caacgccaag acaacttacc ggtctaaaaa accggccaag aatctgaaaa tgcctggcgt
     4201 ctactatgtc gactatcgcc tggaacggat caaggaggct aataacgaga cttacgtgga
     4261 gcagcatgag gtcgctgtcg caagatactg tgatcttcct agcaaacttg gccataagct
     4321 gaacgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     4381 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4441 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4501 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4561 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4621 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4681 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4741 cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag
     4801 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4861 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4921 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg
     4981 atccaatcaa cctctggatt acgcgttaag tcgacaatca acctctggat tacaaaattt
     5041 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5101 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5161 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5221 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5281 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5341 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5401 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5461 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5521 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5581 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     5641 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5701 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5881 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     5941 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6001 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6061 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6121 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6181 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6241 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6301 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6481 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6601 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6661 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6721 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6781 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6841 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6901 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6961 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7081 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7141 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7201 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7261 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7321 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7381 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7441 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7501 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7561 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7621 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7681 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7741 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7801 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7861 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7921 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7981 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8041 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8101 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8161 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8221 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8281 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8341 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8401 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8461 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8521 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8581 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8641 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8701 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8761 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8821 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8881 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8941 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9001 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9061 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9121 taaccctcac taaagggaac aaaagctgga gctgcaagct taa
//
