LOCUS       sequence-473289-       13864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2425
FEATURES             Location/Qualifiers
     source          1..13864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9114..9123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9721..10309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9774..9794)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10192..10203)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10381..10614
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10692..10813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10729..10748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10783..10802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10853..10988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11008..11027)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11009..11027)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11037..11054)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11037..11053)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11046..11068)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11195..11650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11282..11301)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11492..11513
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11676..11780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11781..11849,11850..12641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11999..12018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12812..13400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13301..13320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13554..13571
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13688..13709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13724..13729,13730..13747,13748..13754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13762..13778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13767..13789
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13786..13802
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13786..13802
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13821..13841
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13823..13841
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gacacacccc cctcactcag
     4141 ctgctccgac tgtcagcgcc actttcccag cctcccagag ctctctcggc accgagaact
     4201 gctccatcca tctcccaacc aggacagtga ggaggctgac agcatccctc ggccctaccg
     4261 ttgtcagcag tgtgggcggg gctaccgtca ccccgggagc ctggttaacc atcgtcggac
     4321 ccacgagact ggccttttcc cctgtaccac ctgtggcaag gacttctcca atcccatggc
     4381 tctcaagagc catatgagga cacatgctcc tgagggccgc cgcaggcaca ggcccccacg
     4441 ccccaaggaa gccactccac acctccaggg tgagacggtg tccactgact cctggggcca
     4501 aaggcttggc tctagtgaag gctgggaaaa ccagacaaaa catacagaag agacacctga
     4561 ctgtgaatct gtacctgacc ccagggcagc ttcgggtacg tgggaagatc tgcccaccag
     4621 acaaagagaa ggcttggcaa gccacccagg tcctgaggat ggtgcagacg gctggggacc
     4681 ctccactaac tctgccagag cccctcctct ccccatccca gccagcagcc ttcttagcaa
     4741 cttggaacag tatctggctg aatcagtagt gaacttcaca gggggccagg agcccaccca
     4801 gtcccctcct gctgaggagg agcggcggta caaatgtagt cagtgtggca agacctacaa
     4861 gcacgccggg agcctcacca accaccgcca gagccacacg ctgggcatct acccctgtgc
     4921 catctgtttc aaggagttct ctaacctcat ggctctgaag aaccactctc gactgcatgc
     4981 ccagtatcgg ccttaccact gtccccactg cccccgtgtc ttccggctcc cccgggagct
     5041 gctggaacac cagcagtccc atgagggtga aaggcaggag ccacgctggg aggagaaagg
     5101 gatgcccacc accaatgggc acacagatga gagcagccag gaccagctcc ccagtgcaca
     5161 gatgctgaat ggctctgcgg agctcagcac ctctggggag ctggaggaca gtggcctgga
     5221 ggaataccgg cctttccgct gtggggactg tggccgtact taccgccatg ctgggagcct
     5281 catcaaccat cgaaagagcc accagacagg tgtctacccc tgctcactct gttctaagca
     5341 gctgttcaat gcggctgccc tcaaaaacca tgtgcgggct catcacaggc ccaggcaagg
     5401 agttggggaa aatgggcagc catcagtccc accagctccc ctgctgctgg ctgagaccac
     5461 ccacaaagag gaagaggacc ccaccaccac cctggaccat cggccctata agtgcagtga
     5521 gtgtggtcgt gcttaccgcc accgggggag cctggtgaac catcgccaca gccatcggac
     5581 tggagagtac cagtgctcac tctgtccccg caagtacccc aatctcatgg ccctgcgcaa
     5641 ccacgtgcgg gtacattgca aggctgctcg ccgaagtgca gacatcgggg ctgagggtgc
     5701 ccccagccac ctcaaggtag aactcccgcc tgacccagtg gaggcagagg cagccccgca
     5761 cacagatcag gaccatgtgt gcaaacatga agaagaggcc acggacatca ccccagcagc
     5821 agacaagaca gcagcacata tctgtagcat ctgtgggctg ctctttgaag acgctgagag
     5881 ccttgaacgt catggcctga ctcatggggc aggggaaaag gaaaatagca gaacagagac
     5941 cacaatgtca cctcctaggg cctttgcctg ccgagactgt ggaaagagct atcgccactc
     6001 aggcagcctt atcaaccaca ggcagaccca ccagacagga gacttcagtt gtggggcctg
     6061 tgccaagcac ttccacacca tggctgccat gaagaaccac ttgcgccggc acagtcggcg
     6121 gcggagcagg cggcatcgga agcgggctgg cggtgccagc ggtgggagag aagccaaact
     6181 cctggcagcg gagagctgga cccgggagct agaagacaat gaaggcctgg agtctcccca
     6241 agacccttca ggggaaagtc ctcatggggc tgaaggcaac ctggaaagtg atggggactg
     6301 tttgcaggct gaatctgaag gggacaaatg tgggcttgag agggatgaga cccatttcca
     6361 gggtgataaa gagagcggag gcactgggga aggactggaa aggaaggatg ccagtttact
     6421 tgacaacttg gacatcccag gtgaggaagg tggtggcact cacttctgcg atagcctcac
     6481 tggggtggat gaagaccaga agccagccac tggccaaccc aactcctctt cccactctgc
     6541 caatgctgtc actggctggc aggctggggc cgctcacaca tgctctgact gtgggcattc
     6601 tttcccccat gccactggcc tgctgagcca caggccctgc cacccaccag gcatctatca
     6661 gtgctccctc tgcccgaagg agtttgactc tctgcctgcc ctccgcagcc acttccagaa
     6721 ccataggcct ggggaggcga cctcagcaca gcctttcctc tgctgcctct gtggcatgat
     6781 cttccctggg cgggctggct acaggcttca ccggcgccag gcccacagct cctctggcat
     6841 gactgagggc tcagaggagg agggggaaga ggaaggagtg gcagaggcag cccctgcacg
     6901 cagtccacca ctgcagctct cggaagcaga gctgctgaat cagctgcagc gggaggtgga
     6961 agcgctggac agtgcagggt atgggcacat ctgtggctgc tgtggtcaga cctacgatga
     7021 cctggggagc ctggagcgtc accaccaaag tcagagttct gggactactg cagacaaggc
     7081 tcccagcccc ttgggagtgg caggtgatgc catggagatg gtcgtggaca gtgtcttgga
     7141 ggacatagtg aattctgtct ctggagaggg tggagatgcc aagtctcaag agggagcagg
     7201 cacccccttg ggagacagcc tctgcatcca gggtggggaa agtttgttgg aggctcagcc
     7261 ccgccccttc cgctgcaacc agtgtggcaa gacctatcgc catgggggca gcctggtgaa
     7321 ccaccgcaag atccaccaga ctggagactt tctctgccct gtctgctccc gctgctaccc
     7381 caacctggct gcctaccgta atcatctgcg gaaccaccct cgctgcaaag gctctgagcc
     7441 ccaggttggg cccatcccag aggcagcagg tagcagtgag ctgcaggttg ggcccatccc
     7501 agaaggaggc agcaacaagc cccagcacat ggcagaggag gggccggggc aagcagaagt
     7561 cgagaagctc caggaagaac ttaaagtgga gcccctggag gaagtggcca gggtgaaaga
     7621 agaggtgtgg gaggagacca ctgtgaaggg ggaggagata gagcccaggc tggagactgc
     7681 cgagaagggc tgccagactg aagccagctc tgagcggccc ttcagctgcg aggtgtgtgg
     7741 ccgatcctac aagcacgccg gcagcctcat caaccaccgg cagagccacc agaccggcca
     7801 ctttggctgt caggcctgct ccaagggctt ctcaaacctc atgtccctca agaaccaccg
     7861 gcgcatccat gcagatcccc gacgtttccg ctgcagcgag tgtgggaagg ccttccgcct
     7921 gcggaaacag ctggccagcc accagcgggt ccacatggaa cggcgtgggg gtgggggcac
     7981 ccgaaaggcg actcgggaag atcggccctt ccgctgtggg cagtgcgggc ggacctatcg
     8041 ccacgccggc agcctcctga accaccggcg cagccacgag acgggccagt acagctgccc
     8101 cacctgcccc aagacctact ccaaccgcat ggccctgaag gaccaccaga ggctgcactc
     8161 agagaatcgg cggcgacggg ctggacggtc caggcgcaca gctgtgcgtt gcgccctctg
     8221 tggccgcagc ttccctggcc ggggatcttt ggagcggcac ctgcgggagc atgaggagac
     8281 agaaagggag ccagccaatg gccagggagg cctggatggc acagcggcca gtgaggcgaa
     8341 cctgactggc agccagggac tagagaccca attgggtggt gctgagccag taccccactt
     8401 ggaggatgga gtcccaaggc caggggagcg cagtcagagc cccatcaggg cagcaagctc
     8461 agaagcccca gagccactgt cctggggtgc agggaaggca ggtgggtggc cggtaggtgg
     8521 gggactgggg aatcatagtg gaggctgggt tcctcagttc ctaactaggt cagaggagcc
     8581 agaggacagt gtccacagga gtccttgcca cgctggtgac tgccagctca atggacctac
     8641 tctgagtcac atggatagct gggacaacag agacaacagc tctcagctgc agccagggag
     8701 ccactcctct tgcagccagt gtggcaagac ttactgccag tcaggcagcc tcttgaacca
     8761 caacaccaac aagacagacc gacactattg cctgctctgc tccaaggagt tcttaaatcc
     8821 tgtggccaca aagagccaca gccacaacca catagacgcc cagacctttg cctgtcctga
     8881 ctgtggcaaa gcctttgagt cccaccagga actggccagc cacctgcagg ctcatgcccg
     8941 gggccacagc caggtgccag cccagatgga ggaggccaga gatcccaaag ccgggactgg
     9001 ggaggaccag gtggttctcc ctggtcaagg gaaagcccag gaggccccat cagaaacccc
     9061 cagaggccca ggagagagtg tggagagagc caggggagga caagcggtga cgtccatggc
     9121 ggctgaggac aaggagcggc ccttccgctg cacccagtgc gggcgctcct accgccatgc
     9181 tggcagcctg ctgaaccacc agaaggccca caccacaggg ttgtacccgt gctccctctg
     9241 tcccaaactt ctccctaacc tgctgtctct taagaaccac agcaggaccc acacggaccc
     9301 caagcgccac tgctgcagca tctgtggcaa ggcctttcgg acagctgccc ggctggaggg
     9361 ccacgggcgg gtccatgcac cccgggaggg gcctttcacc tgcccccatt gtccccgcca
     9421 cttccgccgc cgaatcagct tcgtgcagca ccagcagcag caccaggagg agtggacggt
     9481 ggccggctcc ggagccccag tggcaccagt gacgggcaga ggggacttgc cattgccccc
     9541 tccacccacc cccacgaccc cactcctgga tccttcaccc cagtggcctg cagacctcag
     9601 cttctccctc tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     9661 tatatcttgt ggaaaggacg atgcacgcaa cttggctata atgctacgcg ttaagtcgac
     9721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9841 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9901 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9961 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10021 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10081 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    10141 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10201 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10261 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    10321 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    10381 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    10441 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10501 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10561 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    10621 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    10681 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10801 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
    10861 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10921 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10981 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    11041 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    11101 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    11161 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    11221 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    11281 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    11341 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    11401 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    11461 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    11521 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    11581 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    11641 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    11701 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    11761 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    11821 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    11881 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    11941 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    12001 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    12061 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    12121 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    12181 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    12241 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    12301 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    12361 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    12421 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    12481 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    12541 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    12601 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    12661 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    12721 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    12781 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    12841 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    12901 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    12961 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    13021 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    13081 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    13141 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    13201 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    13261 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    13321 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    13381 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    13441 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    13501 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    13561 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    13621 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    13681 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    13741 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    13801 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    13861 aagc
//
