LOCUS       sequence-473307-       10571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Backbone Plasmid, contains 
            Backbone_repABC-pMlb-KanR-TetR_PartTypeBB and an sfGFP dropout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK-BB4 [Backbone_repABC-pMlb-KanR-TetR_PartT...
FEATURES             Location/Qualifiers
     source          1..10571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             964..1758
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1018..1037)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1628..1647
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(6632..7282)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             7388..8587
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     RBS             8906..8917
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8924..9637
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      9649..9720
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9736..9763
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            9916..10025
                     /note="incP origin of transfer"
     CDS             10058..10429
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgcaatag cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg
      661 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
      721 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
      781 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
      841 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
      901 ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt
      961 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     1021 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     1081 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     1141 ctccaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     1201 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     1261 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     1321 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     1381 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     1441 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc
     1501 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     1561 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     1621 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     1681 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     1741 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     1801 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     1861 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1921 tcgcccaccc cccctggcgt tcgtcactcg tttggccatg cggaatctcc agccggaaca
     1981 ccccctcgtc gcccggctgg cggtccgcgc gggattggct tgcgatttca ctttgtcagc
     2041 aagcggtgct gtttgcgcat cgtgacgggt tggtaccgct ggtctattgc cggcccctac
     2101 cccgatagat ttcatccgca ttgacacgac gatgtgttga agccgggaaa tgtgacgagc
     2161 cgtcttcagg agtctttggc ctcggtctgg cgatgctggc cgtcagcatg cgtctcaaca
     2221 tagcggtctt ggcaacacca ccgcgtacct acgatctgaa actttgctcc gccgaatggt
     2281 cgcgtctggt gcgcgcaaat ccatgttttc aatatcgttc tgcgattttt ttactgcggt
     2341 aaatttctgg atgcggtcag tgcgattggc cgcgctctgg ccagcgttaa ccttttgtta
     2401 acccaaaact tcttgccggt aagtccgaat cggagcttaa tggcgcttgt tgtgggcatt
     2461 gcttacaaaa agcgccaatt caggtttcga cgagaatgaa tgtctccatc cggctgccac
     2521 gtttcgagtt cgatgacaaa atcctcgaac agggcgccga gatttcccgg cggttgaacc
     2581 agcttcggat ggagaaattt ccgcccaatg cgacgaagac gctacgcaca ttctcgatgg
     2641 cggaggtcgc tcactacctc ggggtttcgc agaacaattt gaagcgtctc catctcgaag
     2701 gcaaggggcc gttgcctgac caaagtcctt ccggccgtcg ctcctacacg gctacacaga
     2761 tgctcgagct caggcagttc ctggaccagc atggcaggac tgaagtcaag aagtacgttc
     2821 cgcaccgcaa gcttggcgat aagctccagg tggttgcggt ggtcaatttc aaaggcggca
     2881 gcggcaagac gaccacggca gcccacctcg cccagcacct ggcgctgact ggtcatcggg
     2941 ttcttgcgat cgatcttgat ccgcaagctt cgctgtctgc tctgcacggc ttccagcccg
     3001 agctcgatcg caacctctcg ctctatgaag cgatcaggta tgacgacgaa cgcagatcga
     3061 ttgcggatgt gatcataccg acaaatttcc ccggcctcga catcattccc gccaatctcg
     3121 agctgcagga atacgagtat gacacgccgc tcgccatgca agacaaaacc tccagtgccg
     3181 gccgccagtt cttcacgcgc atggccaaag cgttgtcgga ggttgacgat cggtacgatg
     3241 tcgtcgttgt cgattgcccg ccccagcttg gctatctgac tttgacggca atgtcggcgg
     3301 cgacatcggt tctcattacg gtccatccgc agatgctcga cctgatgtcg atgagccagt
     3361 ttcttctgat gcttggcggc atattgaaga ccatcaaggc cgccggcgcc gcgatcgaac
     3421 ttgactggtt tcgctacctg atcacgcgct acgaaccgac cgatattccg caggcgcaga
     3481 tggtgggctt catgcagtcc atgcttgccg gccagatcct ggaaaacccg atgttgaagt
     3541 cgacggcgat ttccgatgca ggcctcacca agcaaacact ttacgaggtg gagaaatcgg
     3601 ccttcacccg ctcgacatat gaccgcgctt tggaatcgct cgacgcggtg aacgccgaga
     3661 tcgtcacact catccatcgt gcgtggggtc ggtcatgacg gcgaacttta cggcagtaaa
     3721 aaccggctat tttgctcgtg gaatcagatc gctagccgct cacggaggcg gttgaatggc
     3781 ccgtaaggat attttcggca gtgtgatggg acccgtcgct gcggtggccg ataacaagga
     3841 tgtatcgggg tatgccgttc gtggtgcctc ccgctcgatc atgcaatcct tcgaggagct
     3901 ctccaagagt tccgtcgtcg atctcgatcc tgccctggtt gacacctcgt tcgtctcgga
     3961 ccgcctggac gaagacgatc aggcgtatca ggagttgctg tcggcaattc gcgagagagg
     4021 tcaagattct ccgattctgg tcagaccgca tccggaggtt tcaggacgct tccagacagt
     4081 attcggccat cgccgcgtca aggttgcccg ccagctggga aggccggtcc gcgccgtcgt
     4141 caagaagatg ggcgacgagg accatgtcat cgcccagggc caggagaatg ccgccagggc
     4201 caatctgtcc ttcatcgaga ggaccctgtt tgccgatcgc attctcaagc gcggccacac
     4261 ctcggacacg gtgaagtccg ctttggcgct cgatgacacg accttttcga agatgcgctc
     4321 cgtgaccggc aatatccccg agccagtcat tgtggctgtt ggctcggcca aggccgtcgg
     4381 acgggacagg tggtggcaac tggccaagct catggagcat cctgggaacg cgggcagggc
     4441 ggccgactat gtgaagagcg ctgatttcgc ggagccaagc agtgacgcac gatttgcgct
     4501 tcttttcgac tacctgaacg ctccggcgaa acgttcgccg gcagcgccga agacacgaga
     4561 aaaggcctgg gcgccgcaag acaggtcggt gcgggccaag atcaccgaca ccggaaaagt
     4621 gttcacgctt gccctgaaag caagggaagc ctcgccgttc ggtgcgttca tcaccgaacg
     4681 gctggacgag cttttcgaag cgttccggca atcggaaatg tctaagagaa caggagactg
     4741 aaaccgcaaa agaaaaaggc ccccgaacgt cgccgtcgcg gaagcccttc tcgtctgtag
     4801 caagctgaga atcgcacttc cccgaatcac tgtcaagagt cgtggcgcca tttcggcgag
     4861 caggtttctt ttgccttcgt gaaaggtgag ggaaatggag agtgggtttg cgacgacgcc
     4921 ttttgggcgg cggccgatga cgcttgcctt ggtgaaggcc aatgccgcgt cgcggcagat
     4981 tccgaaaggg gccgccgttg agaaatggca ggtctaccgg tggctatgcg agggcaagtc
     5041 gatcgtcggc gttggcgatc gtgccctggc ggtgctgagc gggctgttga cgttttatcc
     5101 tgacgaccag atcagcgagg aaaacggctt gatggtcttc ccgtcgaacg cacagctgtc
     5161 tttgcgggcg cacggcatgg ccgattcgac gctgagaagg aacctggccg agctggtgag
     5221 ttgcggcctg atcattcgcc gcgatagtcc gaacggcaaa agattcgctc gtaaggggca
     5281 gggtggtgct gtcatggagg tcttcgggtt ctcgttggcg cctctgctgg cacgggcggc
     5341 ggagtttcag cgcgccgccg agcaggtccg cgccgagtgc caggcgctga aattgatgcg
     5401 ggagcgcatc acgctgcatc gcagggacat tcagaagctg gtcgaggcgg cgatcgagga
     5461 aggggttccg ggcgactggg gcggcgtctg gaggcggttt cggtccatcg tggatgcaat
     5521 tccgcgccgg gcggggctgt cacagcttga agggatcgtt gccgagctgg tcgtgctcca
     5581 cgatgaagtg gataggatgc tggaaagctt cacaagttcc acgaatccta gcggcaatga
     5641 gtcccaaaat gagcggcagc aatctgattc aaacacagac tccatttttg tatttgaacc
     5701 ggctttcgaa aaaagcaggg cggccgttga gccaagaccg gcaccggacg agcggttgaa
     5761 aagctatccg attggactgg tgctgaaggc ctgccccgag tttgcgggct atgctgtcaa
     5821 cgggatcgcc agctggcgcg atttgatgct caccgcggcc caggtgcgcg gctaccttgg
     5881 ggtttcaccg tcggcctatg aggaggcttg ccatgtgatg ggtcaggaag tcgccgcgat
     5941 tgtgattgcg tgtattctgc aacgggcaca gcacatcaat tcggccggtg gctatctgcg
     6001 cgtgctgacg gaaaaggcga gggcagggga gttctcggtc ggaccgatgc tgatggccgc
     6061 gctcaaggcc aatggcccgg caacgaggat gacgggatga ttgaggtaca ggttcgtggg
     6121 gcaggttgct cggcgctcta ggtgacggca tgcggccacc taccggcggt gggctcccgg
     6181 gcttgaaagc tgttcaggaa tgccatatct tttgcacaag aaacatgact cctgatatgg
     6241 tgaacactgt gggccatgca ctcaaggcag ccgagcagaa tgggccgctc gtcttgtcct
     6301 atatggatcg gaacgggaag attgcgatcg agcaggtcgc ggacggtttt ggcatgtcca
     6361 agggtcagtt ggcccagacc gccggtcttg cgcgtgagac actctatcgc tcagagcgca
     6421 gcgctgcggc aaagacccag gggcgtctgc gcgaaatgct cgagatcatc agccgcgtga
     6481 gcgattgggc gggcggcaag gaacaggcga tggcctggta tcgggctcag ccgcttccgg
     6541 catttggcgg acgcactgcc gaagcgctgg tgaaggatgg caaggccgcc gcggtccgtg
     6601 attatctaga tcacatggca gtcggtgtgg ctcaatcgtc accctttctc ggtccttcaa
     6661 cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct
     6721 cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg
     6781 agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct
     6841 cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc
     6901 cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg
     6961 gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct
     7021 gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca
     7081 ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct
     7141 tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc
     7201 gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg
     7261 tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac
     7321 caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag
     7381 gccagacgtg aaacccaaca tacccctgat cgtaattctg agcactgtcg cgctcgacgc
     7441 tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc
     7501 gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg tgcaatttgc
     7561 ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt
     7621 ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt tcctttgggt
     7681 tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc
     7741 ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc
     7801 ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc
     7861 cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg
     7921 tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa
     7981 cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt
     8041 cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga
     8101 ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct
     8161 gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg
     8221 ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac
     8281 acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc
     8341 ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg
     8401 ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat
     8461 ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta
     8521 cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga
     8581 tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt ccggcaagct
     8641 atacgcgccc taggagtgcg gttggaacgt tggcccagcc agatactccc gatcacgagc
     8701 aggacgccga tgatttgaag cgcactcagc gtctgatctt aattaaaacg tgagaccgaa
     8761 agtgaaacgt gatttcatgc gtcattttga acattttgta aatcttattt aataatgtgt
     8821 gcggcaattc acatttaatt tatgaatgtt ttcttaacat cgcggcaact caagaaacgg
     8881 caggttcgga tcttagctac tagagaaaga ggagaaatac tagatgcgta aaggcgaaga
     8941 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
     9001 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
     9061 catctgtact actggtaaac tgccggttcc ttggccgact ctggtaacga cgctgactta
     9121 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
     9181 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
     9241 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
     9301 aggcattgac tttaaagagg acggcaatat cctgggccat aagctggaat acaattttaa
     9361 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
     9421 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
     9481 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
     9541 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
     9601 agcgggcatc acgcatggta tggatgaact gtacaaatga ccaggcatca aataaaacga
     9661 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     9721 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag gtctctgagt
     9781 atcttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     9841 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     9901 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     9961 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
    10021 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
    10081 caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga
    10141 ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg
    10201 ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg
    10261 cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc
    10321 gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga
    10381 gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc
    10441 gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga
    10501 agaggcactt cgagctgtaa gtacatcacc gacgagcaag gcaagacgat cccgaagatc
    10561 aaaggatctt c
//
