LOCUS       sequence-47331-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pMito-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(135..845)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(413..432)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      842..851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgcataacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      121 agagtcgcgg ccgcttactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg
      181 gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg
      241 atgtcgacga tgtaggcgcc gggcaactgc acgggcttct tggccttgta ggtggtcttg
      301 acctcggagg tgtagtggcc gccgtccttc agcttcagcc tgagcttgtc ctcgcccttc
      361 agggcgccgt cctcggggta catccgctcg gaggaggcct ccatgcccat ggtcttcttc
      421 tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac
      481 tcgccgtcct gcagggagga gtcctgagtc acggtcacca cgccgccgtc ctcgaagttc
      541 atcacgcgct cccacttgaa gccctcgggg aaggacagct tgaggtagtc ggggatgtcg
      601 gcggggtgct tcacgtaggc cttggagccg taggtgaact gaggggacaa gatgtcccag
      661 gcgaagggca gggggccacc cttggtcacc ttcagcttaa cggtctgaaa gccctcgtag
      721 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
      781 cgcaccttga agcgcatgaa ctccttgatg atggccatgt tattctcctc gcccttgctc
      841 accatggtgg cgaccggtgg atcccccaac gaatggatct tggcgcgcgg cactgggagc
      901 cgccgggccg agcctgtcaa gccccgcagc agcagc
//
