LOCUS       sequence-47332-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pMito-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..85
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             119..829
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     532..551
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg
       61 gcggctccca gtgccgcgcg ccaagatcca ttcgttgggg gatccaccgg tcgccaccat
      121 ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt
      181 gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      241 cccctacgag ggctttcaga ccgttaagct gaaggtgacc aagggtggcc ccctgccctt
      301 cgcctgggac atcttgtccc ctcagttcac ctacggctcc aaggcctacg tgaagcaccc
      361 cgccgacatc cccgactacc tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      421 gatgaacttc gaggacggcg gcgtggtgac cgtgactcag gactcctccc tgcaggacgg
      481 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      541 gcagaagaag accatgggca tggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      601 cctgaagggc gaggacaagc tcaggctgaa gctgaaggac ggcggccact acacctccga
      661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagttg cccggcgcct acatcgtcga
      721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac
      841 tctagatcat aatcagccat accacatttg tagaggtttt actt
//
