LOCUS       sequence-47335-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pH2B-LSSmOrange
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..837)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(405..424)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat ctagagtcgc
      121 ggccgcttac ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc
      181 gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgtcgac
      241 gatgtaggcg ccgggcaact gcacgggctt cttggccttg taggtggtct tgacctcgga
      301 ggtgtagtgg ccgccgtcct tcagcttcag cctgagcttg tcctcgccct tcagggcgcc
      361 gtcctcgggg tacatccgct cggaggaggc ctccatgccc atggtcttct tctgcattac
      421 ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc
      481 ctgcagggag gagtcctgag tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg
      541 ctcccacttg aagccctcgg ggaaggacag cttgaggtag tcggggatgt cggcggggtg
      601 cttcacgtag gccttggagc cgtaggtgaa ctgaggggac aagatgtccc aggcgaaggg
      661 cagggggcca cccttggtca ccttcagctt aacggtctga aagccctcgt aggggcggcc
      721 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgcgcacctt
      781 gaagcgcatg aactccttga tgacggccat gttattctcc tcgcccttgc tcaccatggt
      841 ggcgaccggt ggatccttag cgctggtgta cttggtgatg gccttagtac cctcggacac
      901 ggcgtgcttg gccaactccc caggcagcag c
//
